Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RI032_RS04105 Genome accession   NZ_CP140571
Coordinates   863106..864200 (-) Length   364 a.a.
NCBI ID   WP_049410297.1    Uniprot ID   -
Organism   Stenotrophomonas geniculata strain FLMAT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 858106..869200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI032_RS04080 - 859306..859851 (+) 546 WP_071306280.1 DNA-3-methyladenine glycosylase I -
  RI032_RS04085 - 859937..860644 (-) 708 WP_029379885.1 YitT family protein -
  RI032_RS04090 - 860755..860967 (-) 213 WP_049410257.1 DUF4287 domain-containing protein -
  RI032_RS04095 - 861061..861957 (+) 897 WP_043398312.1 LysR family transcriptional regulator -
  RI032_RS04100 - 862000..862983 (-) 984 WP_164191942.1 aldo/keto reductase -
  RI032_RS04105 pilU 863106..864200 (-) 1095 WP_049410297.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RI032_RS04110 pilT 864348..865385 (-) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  RI032_RS04115 - 865473..866150 (+) 678 WP_228355123.1 YggS family pyridoxal phosphate-dependent enzyme -
  RI032_RS04120 proC 866169..866990 (+) 822 WP_322547899.1 pyrroline-5-carboxylate reductase -
  RI032_RS04125 - 867050..867925 (-) 876 WP_057503301.1 LysR family transcriptional regulator -
  RI032_RS04130 - 868024..868626 (+) 603 WP_010483701.1 short chain dehydrogenase -
  RI032_RS04135 soxR 868700..869134 (-) 435 WP_019336938.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40583.64 Da        Isoelectric Point: 6.7680

>NTDB_id=914735 RI032_RS04105 WP_049410297.1 863106..864200(-) (pilU) [Stenotrophomonas geniculata strain FLMAT1]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=914735 RI032_RS04105 WP_049410297.1 863106..864200(-) (pilU) [Stenotrophomonas geniculata strain FLMAT1]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAAT
TCGAGAAGACCCACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGTCGTTTCCGCGTGAGCTGCTTCTACCAGCGC
AACCAGGTGGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAGTTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCCACCGGCACCGGCAAGTCAACGTCGCTGGCGGCGA
TGATCGGTTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAG
GGCTGCATCATCACCCAGCGTGAAGTCGGCATCGATACCGACAGCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATCGGCGAAGTGCGTACCCGCGAAGGCATGGACCATGCCATCGCGTTCGCCGAAACCGGCCACC
TGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAATCTCAAAGGCGTGGTCGCTCAGCAGCTGATTCCTTCGCCCGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGATGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAG
ATCAGCTACGAGGACGCACTGCGCTACGCCGATTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGTGA
CGCGCGCACCCTGTCGCAGGGGCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.436

98.352

0.673
  pilU Acinetobacter baylyi ADP1

64.368

95.604

0.615
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.923

89.286

0.365