Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R5R73_RS00785 Genome accession   NZ_CP140261
Coordinates   173151..174185 (+) Length   344 a.a.
NCBI ID   WP_071232316.1    Uniprot ID   A0A1S2DBR7
Organism   Salinicola sp. LHM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 168151..179185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5R73_RS00755 (R5R73_00755) sfsA 168210..168974 (+) 765 WP_071232322.1 DNA/RNA nuclease SfsA -
  R5R73_RS00760 (R5R73_00760) metW 168971..169567 (-) 597 WP_110636089.1 methionine biosynthesis protein MetW -
  R5R73_RS00765 (R5R73_00765) metX 169567..170724 (-) 1158 WP_322528494.1 homoserine O-succinyltransferase MetX -
  R5R73_RS00770 (R5R73_00770) - 170890..171480 (-) 591 WP_071232319.1 YggT family protein -
  R5R73_RS00775 (R5R73_00775) proC 171494..172333 (-) 840 WP_322528495.1 pyrroline-5-carboxylate reductase -
  R5R73_RS00780 (R5R73_00780) - 172400..173110 (-) 711 WP_071232317.1 YggS family pyridoxal phosphate-dependent enzyme -
  R5R73_RS00785 (R5R73_00785) pilT 173151..174185 (+) 1035 WP_071232316.1 type IV pilus twitching motility protein PilT Machinery gene
  R5R73_RS00790 (R5R73_00790) pilU 174220..175353 (+) 1134 WP_071232315.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R5R73_RS00795 (R5R73_00795) - 175383..176498 (-) 1116 WP_071232314.1 anhydro-N-acetylmuramic acid kinase -
  R5R73_RS00800 (R5R73_00800) tyrS 176647..177846 (+) 1200 WP_071232488.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37544.92 Da        Isoelectric Point: 6.7057

>NTDB_id=913305 R5R73_RS00785 WP_071232316.1 173151..174185(+) (pilT) [Salinicola sp. LHM]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDMRRLNVPALDDGQVRRLIYDVMNDRQRRDFEAALETDFSFEVTGVSR
FRVNAFTQGRGAGAVFRTIPSNVMTLDDLGLGDVFRRLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINSNRQEHILTIED
PIEFVHESKRCLINQREVHRDTKGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKGMVRSMLSESLQAVISQTLLKREGGGRVAAHEILVATSAIRNLIREGKVAQMYSAIQTGGGLGMQTLDAAL
SRLVTDGVVTRQEAHAKSRAGLDQ

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=913305 R5R73_RS00785 WP_071232316.1 173151..174185(+) (pilT) [Salinicola sp. LHM]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAGCAAGGCGCCTCCGACCTGCACCTTTCGGCGGGATTGCCTCCCAT
GATACGGGTCGACGGTGACATGCGTCGTCTCAACGTGCCGGCGCTCGACGACGGCCAGGTACGTCGTCTGATCTACGACG
TCATGAACGATCGACAGCGCCGCGACTTCGAAGCCGCGCTGGAAACCGACTTTTCTTTCGAAGTCACCGGTGTATCGCGT
TTTCGTGTCAATGCGTTCACGCAAGGGCGCGGCGCCGGCGCGGTCTTCCGTACCATTCCCAGCAATGTCATGACGCTCGA
CGATCTCGGCCTGGGGGATGTCTTCCGCCGGTTGTCGATGCTGCCGCGCGGCCTGGTACTGGTGACGGGGCCGACCGGCA
GCGGCAAGTCGACGACGCTGGCGGCGATGATCGACTACATCAACAGCAATCGCCAGGAACACATCCTCACGATCGAGGAC
CCGATCGAATTCGTGCACGAGAGCAAGCGCTGCCTGATCAACCAGCGAGAGGTGCATCGCGATACCAAAGGCTTCGCGCC
AGCGCTACGGTCGGCGCTGCGCGAGGATCCGGATGTCATCCTGGTGGGCGAGCTGCGAGATCTGGAAACCATTCGGCTTG
CATTGACGGCGGCCGAGACCGGGCATCTGGTGTTCGGCACCCTGCATACCACCTCGGCGGCCAAGACCATCGACCGGATC
ATCGATGTCTTCCCCGGTGACGAGAAGGGCATGGTGCGTTCGATGCTGTCGGAATCTCTGCAAGCGGTGATCTCGCAGAC
GTTGCTCAAGCGCGAGGGCGGTGGCCGTGTCGCGGCCCACGAGATTCTCGTGGCCACCTCGGCGATCCGCAATCTGATTC
GGGAAGGCAAGGTCGCGCAGATGTATTCGGCGATTCAGACGGGCGGCGGCCTGGGCATGCAGACACTGGATGCGGCGCTG
TCCCGATTGGTGACGGATGGTGTGGTGACGCGCCAGGAAGCCCATGCCAAATCGCGTGCCGGGCTCGATCAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2DBR7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.466

98.547

0.773
  pilT Pseudomonas aeruginosa PAK

78.466

98.547

0.773
  pilT Acinetobacter nosocomialis M2

76.401

98.547

0.753
  pilT Acinetobacter baumannii D1279779

76.401

98.547

0.753
  pilT Acinetobacter baumannii strain A118

76.401

98.547

0.753
  pilT Acinetobacter baylyi ADP1

75.294

98.837

0.744
  pilT Legionella pneumophila strain Lp02

70.149

97.384

0.683
  pilT Legionella pneumophila strain ERS1305867

70.149

97.384

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

65

98.837

0.642
  pilT Neisseria gonorrhoeae MS11

64.706

98.837

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.381

96.221

0.523
  pilU Pseudomonas stutzeri DSM 10701

43.478

100

0.436
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41