Detailed information NTG0913306

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5R73_RS00790
• Genome accession: NZ_CP140261
• Coordinates: 174220..175353 (+)
• Length: 377 a.a.
• NCBI ID: WP_071232315.1
• Uniprot ID: A0A1S2DCL5
• Organism: Salinicola sp. LHM
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 169220..180353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5R73_RS00765 (R5R73_00765)	metX	169567..170724 (-)	1158	WP_322528494.1	homoserine O-succinyltransferase MetX	-
R5R73_RS00770 (R5R73_00770)	-	170890..171480 (-)	591	WP_071232319.1	YggT family protein	-
R5R73_RS00775 (R5R73_00775)	proC	171494..172333 (-)	840	WP_322528495.1	pyrroline-5-carboxylate reductase	-
R5R73_RS00780 (R5R73_00780)	-	172400..173110 (-)	711	WP_071232317.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5R73_RS00785 (R5R73_00785)	pilT	173151..174185 (+)	1035	WP_071232316.1	type IV pilus twitching motility protein PilT	Machinery gene
R5R73_RS00790 (R5R73_00790)	pilU	174220..175353 (+)	1134	WP_071232315.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R5R73_RS00795 (R5R73_00795)	-	175383..176498 (-)	1116	WP_071232314.1	anhydro-N-acetylmuramic acid kinase	-
R5R73_RS00800 (R5R73_00800)	tyrS	176647..177846 (+)	1200	WP_071232488.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41722.82 Da
• Isoelectric Point: 6.0605

>NTDB_id=913306 R5R73_RS00790 WP_071232315.1 174220..175353(+) (pilU) [Salinicola sp. LHM]
MTPSQWFEQLLDIMLAKQASDLLISTGAPPCLKLDGKLVALGDVPLQPDQVRELVAIALPQGRREAFETEHEANFALSLS
GKGRFRVSAFFQRSQPAMVVRRIAFDIPDLESLGLPEVTGTLASIRRGLVLVVGGTGSGKSTTLASMIQHRNRTVGGHII
CIEDPIEYMHPHQRGIINQREVGIDTESYEVALKNALRQAPDVIVIGEIRSRDTMELALSFAETGHLCLATFHANNADQT
IERILSLFPPSRQEQIGMDLSLNLRAVVAQRLLPRQDGSGRCLATEVLLKSPLISELIRRGEVGELKDLMRRSRELGMQT
FDQALYELYRRGDISEGLALAHADSANDLRMLIKYGERHLLGDRGDDGTRFSLVDDQ

Nucleotide

• Length: 1134 bp

>NTDB_id=913306 R5R73_RS00790 WP_071232315.1 174220..175353(+) (pilU) [Salinicola sp. LHM]
ATGACCCCCTCTCAATGGTTTGAACAACTCCTCGACATCATGCTGGCCAAGCAGGCTTCGGACCTGCTGATCTCTACCGG
TGCGCCTCCGTGTCTGAAACTGGATGGCAAGCTGGTGGCGCTGGGCGATGTGCCGCTGCAACCGGATCAGGTACGTGAGC
TGGTCGCCATCGCCCTGCCCCAGGGGCGTCGGGAGGCGTTCGAGACGGAGCATGAGGCCAATTTCGCTCTGAGCCTGAGC
GGCAAGGGGCGTTTTCGCGTCAGCGCCTTCTTCCAGCGCAGCCAGCCGGCAATGGTCGTTCGGCGGATCGCTTTCGATAT
TCCCGATCTCGAATCGCTGGGGCTGCCGGAAGTGACGGGAACGCTGGCTTCCATTCGACGCGGGCTGGTGCTGGTGGTCG
GCGGGACCGGCAGCGGCAAGTCGACGACGCTGGCGTCGATGATCCAGCACCGCAATCGGACGGTCGGCGGCCATATCATC
TGTATCGAAGATCCCATCGAATACATGCACCCGCACCAGCGTGGCATCATCAATCAGCGGGAAGTGGGTATCGATACCGA
GTCCTACGAAGTCGCGCTGAAGAACGCCCTGCGCCAGGCTCCCGACGTGATCGTGATCGGCGAGATCAGAAGCCGCGATA
CCATGGAGCTGGCGCTCAGCTTTGCCGAAACCGGGCATCTCTGTCTGGCCACTTTCCATGCCAACAACGCCGACCAGACG
ATCGAGCGCATTCTCAGTCTGTTCCCGCCGTCCCGGCAGGAGCAGATCGGGATGGACCTTTCGCTCAACCTGCGCGCTGT
GGTCGCTCAGCGGCTGCTGCCGCGTCAAGACGGCTCCGGGCGCTGCTTGGCGACCGAGGTTCTGCTCAAGTCCCCCTTGA
TCAGCGAATTGATCCGGCGCGGTGAGGTGGGGGAGTTGAAGGACCTGATGCGGCGCTCCCGCGAGCTGGGCATGCAGACC
TTCGACCAGGCGCTCTATGAACTCTATCGGCGCGGGGATATCAGTGAGGGGCTGGCGCTGGCCCATGCCGATTCGGCCAA
TGACCTGCGCATGCTGATCAAGTATGGCGAGCGCCATCTTCTCGGGGATCGAGGGGACGATGGCACACGATTTTCACTGG
TCGACGATCAGTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-274)

3D structure

• AlphaFold DB: A0A1S2DCL5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Vibrio cholerae strain A1552	57.341	95.756	0.549
pilU	Pseudomonas stutzeri DSM 10701	58.571	92.838	0.544
pilU	Acinetobacter baylyi ADP1	54.83	93.369	0.512
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	46.224	87.798	0.406
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	42.593	85.942	0.366
pilT	Vibrio cholerae strain A1552	42.593	85.942	0.366