Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SR894_RS01600 Genome accession   NZ_CP140255
Coordinates   325894..326937 (+) Length   347 a.a.
NCBI ID   WP_133731567.1    Uniprot ID   -
Organism   Vreelandella neptunia strain ATCC BAA-805     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320894..331937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR894_RS01570 (SR894_01570) - 321234..321683 (-) 450 WP_133731572.1 DUF4426 domain-containing protein -
  SR894_RS01575 (SR894_01575) metW 321725..322318 (-) 594 WP_133731731.1 methionine biosynthesis protein MetW -
  SR894_RS01580 (SR894_01580) - 322318..323544 (-) 1227 WP_133731571.1 homoserine O-acetyltransferase -
  SR894_RS01585 (SR894_01585) - 323614..324207 (-) 594 WP_133731570.1 YggT family protein -
  SR894_RS01590 (SR894_01590) proC 324265..325089 (-) 825 WP_133731569.1 pyrroline-5-carboxylate reductase -
  SR894_RS01595 (SR894_01595) - 325145..325861 (-) 717 WP_133731568.1 YggS family pyridoxal phosphate-dependent enzyme -
  SR894_RS01600 (SR894_01600) pilT 325894..326937 (+) 1044 WP_133731567.1 type IV pilus twitching motility protein PilT Machinery gene
  SR894_RS01605 (SR894_01605) pilU 326930..328111 (+) 1182 WP_133731566.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SR894_RS01610 (SR894_01610) - 328163..329302 (-) 1140 WP_133731565.1 anhydro-N-acetylmuramic acid kinase -
  SR894_RS01615 (SR894_01615) tyrS 329431..330630 (+) 1200 WP_035582992.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38158.65 Da        Isoelectric Point: 6.0078

>NTDB_id=913265 SR894_RS01600 WP_133731567.1 325894..326937(+) (pilT) [Vreelandella neptunia strain ATCC BAA-805]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAIDNNDVRKLIYDIMNDRQRRDYEEHLEIDFSFEVPGIAR
FRVNAFTQARGAGAVFRTIPNQVLSMQTLGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYEHILTIED
PVEFVHASKRCLINQREVHRDTHSFADALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVVSQSLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQMYSAIQTGGSLGMQTLDAAL
SRLVKDGTVSLEEAQLSAKGTLAIKDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=913265 SR894_RS01600 WP_133731567.1 325894..326937(+) (pilT) [Vreelandella neptunia strain ATCC BAA-805]
ATGGATATTACCGAACTGCTAGCATTCTCGGCAAAGCAGAATGCCTCTGACTTGCACCTTTCGGCCGGTTTGCCGCCCAT
GATACGTGTTGATGGTGACATTCGTCGGCTCAATGTGCCCGCTATCGATAATAATGATGTGCGCAAATTGATCTACGACA
TCATGAATGATCGTCAGCGGCGCGACTACGAAGAGCACCTGGAAATTGATTTTTCCTTTGAAGTGCCGGGGATCGCCCGC
TTCCGCGTTAACGCCTTTACGCAAGCGCGCGGGGCGGGAGCCGTGTTTCGCACCATTCCTAATCAGGTGCTCTCCATGCA
GACCCTGGGGCTCGGTGAGGTGTTCGAGCGCCTGGCGATGTTGCCGCGGGGCCTGGTGCTGGTGACTGGGCCAACGGGGT
CGGGCAAGAGCACCACGCTGGCCGCGATGATCGATTACATTAACGATCATCGTTACGAGCATATTCTCACCATTGAAGAT
CCCGTTGAATTTGTCCACGCCAGCAAGCGCTGTTTGATTAACCAACGCGAAGTGCATCGCGACACCCATAGCTTTGCCGA
TGCGTTGCGCAGCGCGCTGCGTGAAGACCCGGATGTGATCCTGGTCGGCGAGCTGCGTGATCTGGAGACCATCCGTTTAG
CATTGACCGCGGCTGAAACCGGCCATCTGGTGCTGGGGACGCTGCATACCACTTCTGCTGCCAAGACAATCGATCGGATC
ATTGATGTTTTTCCCGGTGAAGAGAAAGCCATGGTGCGCTCCATGCTCTCAGAATCACTCCAGGCAGTGGTGTCGCAATC
GCTGCTTAAACGTCAGGGCGGCGGACGCGTAGCGGCCCATGAGATCCTCATTGCCACTTCTGCGGTGCGCAACTTGATCC
GCGAAGACAAAGTGGCGCAGATGTACTCGGCCATTCAGACCGGCGGCAGCCTGGGCATGCAAACCCTGGATGCCGCGCTG
TCTCGGTTAGTGAAAGACGGTACGGTAAGTTTGGAAGAGGCGCAGCTGAGTGCGAAGGGAACGCTGGCGATAAAAGATGA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

78.171

97.695

0.764
  pilT Pseudomonas stutzeri DSM 10701

77.581

97.695

0.758
  pilT Acinetobacter nosocomialis M2

75.811

97.695

0.741
  pilT Acinetobacter baumannii D1279779

75.811

97.695

0.741
  pilT Acinetobacter baumannii strain A118

75.811

97.695

0.741
  pilT Acinetobacter baylyi ADP1

75.221

97.695

0.735
  pilT Legionella pneumophila strain Lp02

72.189

97.406

0.703
  pilT Legionella pneumophila strain ERS1305867

72.189

97.406

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.149

96.542

0.677
  pilT Vibrio cholerae strain A1552

70.149

96.542

0.677
  pilT Neisseria meningitidis 8013

65.205

98.559

0.643
  pilT Neisseria gonorrhoeae MS11

64.912

98.559

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.828

93.948

0.524
  pilU Pseudomonas stutzeri DSM 10701

42.773

97.695

0.418
  pilU Vibrio cholerae strain A1552

41.593

97.695

0.406
  pilU Acinetobacter baylyi ADP1

40.774

96.83

0.395