Detailed information NTG0913266

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SR894_RS01605
• Genome accession: NZ_CP140255
• Coordinates: 326930..328111 (+)
• Length: 393 a.a.
• NCBI ID: WP_133731566.1
• Uniprot ID: -
• Organism: Vreelandella neptunia strain ATCC BAA-805
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 321930..333111

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SR894_RS01580 (SR894_01580)	-	322318..323544 (-)	1227	WP_133731571.1	homoserine O-acetyltransferase	-
SR894_RS01585 (SR894_01585)	-	323614..324207 (-)	594	WP_133731570.1	YggT family protein	-
SR894_RS01590 (SR894_01590)	proC	324265..325089 (-)	825	WP_133731569.1	pyrroline-5-carboxylate reductase	-
SR894_RS01595 (SR894_01595)	-	325145..325861 (-)	717	WP_133731568.1	YggS family pyridoxal phosphate-dependent enzyme	-
SR894_RS01600 (SR894_01600)	pilT	325894..326937 (+)	1044	WP_133731567.1	type IV pilus twitching motility protein PilT	Machinery gene
SR894_RS01605 (SR894_01605)	pilU	326930..328111 (+)	1182	WP_133731566.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SR894_RS01610 (SR894_01610)	-	328163..329302 (-)	1140	WP_133731565.1	anhydro-N-acetylmuramic acid kinase	-
SR894_RS01615 (SR894_01615)	tyrS	329431..330630 (+)	1200	WP_035582992.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 393 a.a.
• Molecular weight: 43381.53 Da
• Isoelectric Point: 6.3617

>NTDB_id=913266 SR894_RS01605 WP_133731566.1 326930..328111(+) (pilU) [Vreelandella neptunia strain ATCC BAA-805]
MNKDSSIQAPSVELSPTQWLHELLDIMVEQNASDLLISVGAPPSLKMPDGLVPLGQQRLTTHQVNELVTHALPEGLRERF
ASEREANFALSREGKGRFRVSAFQQRNQMAMVVRSIAIEIPRLDALGVPEQLAELAQAKRGLVLVVGGTGTGKSTTLAAM
IQQRNETVGGHIISIEDPIEYLHPHKRAIVNQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHL
CLATLHANNANQALERIIHFFPHERHEQIWMDLSLNLRAVVAQQLLPTLAGGRRAAIEIMLQSPRIADLIRKGEVAEIKA
AMAKSRDAGMQTFDQALHDLHQAGHISREVAMAHADSANDLRMLLNVSDAKGRNLSLDEQVPSHLGLRDGDDY

Nucleotide

• Length: 1182 bp

>NTDB_id=913266 SR894_RS01605 WP_133731566.1 326930..328111(+) (pilU) [Vreelandella neptunia strain ATCC BAA-805]
ATGAATAAAGACAGTTCAATTCAAGCGCCGTCTGTCGAGCTTTCGCCTACTCAATGGCTGCATGAGCTGCTCGATATTAT
GGTTGAGCAGAACGCCTCCGATCTGCTCATCTCTGTGGGCGCGCCGCCAAGCCTAAAAATGCCCGATGGCCTGGTGCCGC
TGGGTCAGCAGCGTTTAACCACCCATCAGGTTAATGAACTGGTCACCCATGCGCTGCCGGAAGGGTTGCGGGAACGTTTT
GCCAGTGAGCGAGAGGCGAATTTTGCCCTCAGTCGCGAGGGCAAAGGGCGTTTTCGGGTCAGCGCGTTTCAGCAGCGCAA
CCAGATGGCCATGGTGGTACGCAGCATCGCGATTGAAATTCCTCGCTTGGATGCACTGGGCGTACCGGAGCAGTTGGCAG
AATTAGCTCAGGCCAAGCGTGGGCTGGTGCTGGTGGTGGGCGGCACGGGAACGGGTAAATCAACGACCCTGGCGGCGATG
ATTCAGCAGCGTAACGAGACCGTCGGTGGGCATATCATCAGCATCGAAGACCCGATTGAGTATTTGCACCCGCATAAGCG
CGCCATAGTGAATCAGCGCGAAGTGGGGATTGATACCGAGTCGTTTGAGGTGGCGCTTAAAAACACCCTGCGCCAGGCGC
CGGATGTGATTCTGATTGGTGAAATCCGCACTCGGGAGACCATGGAACACGCGCTGACATTTGCTGAAACCGGTCACCTC
TGTTTGGCGACACTGCATGCCAATAACGCTAACCAGGCGCTGGAGCGCATCATTCACTTCTTTCCCCATGAACGCCATGA
GCAGATATGGATGGATCTTTCGTTGAATCTGCGTGCGGTCGTCGCCCAGCAACTGCTGCCCACGCTTGCTGGTGGGCGCC
GTGCGGCTATTGAGATCATGCTGCAGTCGCCGCGCATTGCCGACTTGATCCGTAAAGGGGAGGTCGCCGAGATTAAAGCA
GCGATGGCCAAGTCCCGCGATGCCGGGATGCAAACCTTTGATCAGGCGCTGCATGACCTGCATCAGGCGGGGCATATCAG
TAGGGAGGTGGCCATGGCTCACGCCGACTCGGCTAACGACCTGCGAATGCTGCTCAACGTCAGTGACGCCAAGGGGCGTA
ACCTGTCACTAGATGAACAGGTGCCTAGCCATCTAGGGCTGCGCGATGGCGATGATTACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (85-287)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.4	95.42	0.557
pilU	Vibrio cholerae strain A1552	57.714	89.059	0.514
pilU	Acinetobacter baylyi ADP1	55.307	91.094	0.504
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	92.621	0.377