Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SR900_RS00975 Genome accession   NZ_CP140158
Coordinates   206820..207854 (-) Length   344 a.a.
NCBI ID   WP_018623452.1    Uniprot ID   -
Organism   Kangiella aquimarina strain DSMZ 16071     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 201820..212854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR900_RS00955 (SR900_00955) - 202181..202741 (+) 561 WP_018623448.1 YqgE/AlgH family protein -
  SR900_RS00960 (SR900_00960) ruvX 202741..203178 (+) 438 WP_018623449.1 Holliday junction resolvase RuvX -
  SR900_RS00965 (SR900_00965) - 203293..205554 (+) 2262 WP_018623450.1 EAL domain-containing protein -
  SR900_RS00970 (SR900_00970) pilU 205671..206810 (-) 1140 WP_018623451.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SR900_RS00975 (SR900_00975) pilT 206820..207854 (-) 1035 WP_018623452.1 type IV pilus twitching motility protein PilT Machinery gene
  SR900_RS00980 (SR900_00980) - 207893..208576 (+) 684 WP_026309171.1 YggS family pyridoxal phosphate-dependent enzyme -
  SR900_RS00985 (SR900_00985) proC 208704..209561 (+) 858 WP_018623454.1 pyrroline-5-carboxylate reductase -
  SR900_RS00990 (SR900_00990) - 209584..210171 (+) 588 WP_018623455.1 YggT family protein -
  SR900_RS00995 (SR900_00995) - 210318..210767 (+) 450 WP_018623456.1 DUF4426 domain-containing protein -
  SR900_RS01000 (SR900_01000) rdgB 210882..211514 (+) 633 WP_018623457.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SR900_RS01005 (SR900_01005) hemW 211520..212752 (+) 1233 WP_018623458.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38350.02 Da        Isoelectric Point: 6.7306

>NTDB_id=913154 SR900_RS00975 WP_018623452.1 206820..207854(-) (pilT) [Kangiella aquimarina strain DSMZ 16071]
MDITELLAFSVKNNASDLHISAGMPPLIRVDGDIRRINVPPLEDKEVHSLIYDIMNDKQRKDYEEFLEADFSFELKDLAR
FRVNAFVQNRGRAAVFRTIPSRVLTLEELNAPKIFQDISLEPRGLVLVTGPTGSGKSTTLAGMIDYVNENKHDHILTIED
PIEFVHESKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAGEKAMVRSMLSESLQAVIAQTLLKKNGGGRVAAHEILVGTPAIRNLIREDKVAQMYSAMQTGLSLGMQTLDHSL
KQLVSKGVVSRTDARMKAKNKEEF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=913154 SR900_RS00975 WP_018623452.1 206820..207854(-) (pilT) [Kangiella aquimarina strain DSMZ 16071]
ATGGATATTACAGAGCTTTTAGCTTTCAGTGTAAAGAATAATGCATCGGATTTGCATATTTCAGCGGGCATGCCGCCGCT
TATTCGTGTTGATGGCGATATTCGACGAATCAATGTGCCGCCATTGGAAGACAAGGAAGTACACTCGTTAATCTACGATA
TCATGAACGACAAGCAGCGAAAGGATTATGAAGAATTCCTTGAAGCTGACTTCTCATTCGAACTTAAAGATTTAGCCCGT
TTCCGTGTTAATGCTTTCGTGCAAAACCGCGGACGAGCTGCCGTATTTCGTACCATTCCATCTCGTGTATTAACACTTGA
AGAGTTGAATGCACCAAAAATTTTCCAGGATATTTCATTAGAACCTCGCGGACTGGTGTTGGTTACGGGGCCTACGGGTT
CAGGTAAATCAACCACCTTGGCAGGGATGATCGATTATGTGAATGAAAACAAGCATGATCACATTCTAACCATTGAGGAT
CCGATCGAGTTCGTTCACGAAAGTAAAAAGTGTCTGGTAAACCAACGTGAGGTGCACCGTGATACTCACAGCTTCTCTAA
CGCACTTCGTTCAGCGCTTCGTGAAGACCCTGATATCATTCTGGTTGGTGAGATGCGTGACCTTGAAACCATTCGCTTAG
CGTTAACTGCGGCAGAAACCGGTCACCTGGTATTTGGTACCCTGCATACAACCTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTTTTCCCTGCTGGAGAAAAAGCAATGGTGCGCTCAATGTTGTCAGAGTCACTGCAAGCAGTTATCGCACAGAC
CCTGTTGAAGAAAAATGGTGGCGGTCGTGTAGCAGCCCATGAAATTCTGGTTGGTACGCCAGCTATTCGTAACCTCATTC
GTGAAGATAAAGTTGCACAGATGTACTCAGCAATGCAGACCGGTTTATCGCTGGGCATGCAGACGCTTGATCACAGTTTG
AAGCAGTTGGTCAGCAAAGGTGTTGTGTCACGCACAGATGCTCGAATGAAAGCCAAGAACAAAGAAGAGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.395

100

0.814
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

73.837

100

0.738
  pilT Legionella pneumophila strain ERS1305867

73.837

100

0.738
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.965

0.689
  pilT Vibrio cholerae strain A1552

70.326

97.965

0.689
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Vibrio cholerae strain A1552

43.284

97.384

0.422
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.41
  pilU Acinetobacter baylyi ADP1

39.881

97.674

0.39