Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SR900_RS00970 Genome accession   NZ_CP140158
Coordinates   205671..206810 (-) Length   379 a.a.
NCBI ID   WP_018623451.1    Uniprot ID   -
Organism   Kangiella aquimarina strain DSMZ 16071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 200671..211810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR900_RS00950 (SR900_00950) - 201210..202133 (+) 924 WP_018623447.1 energy transducer TonB -
  SR900_RS00955 (SR900_00955) - 202181..202741 (+) 561 WP_018623448.1 YqgE/AlgH family protein -
  SR900_RS00960 (SR900_00960) ruvX 202741..203178 (+) 438 WP_018623449.1 Holliday junction resolvase RuvX -
  SR900_RS00965 (SR900_00965) - 203293..205554 (+) 2262 WP_018623450.1 EAL domain-containing protein -
  SR900_RS00970 (SR900_00970) pilU 205671..206810 (-) 1140 WP_018623451.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SR900_RS00975 (SR900_00975) pilT 206820..207854 (-) 1035 WP_018623452.1 type IV pilus twitching motility protein PilT Machinery gene
  SR900_RS00980 (SR900_00980) - 207893..208576 (+) 684 WP_026309171.1 YggS family pyridoxal phosphate-dependent enzyme -
  SR900_RS00985 (SR900_00985) proC 208704..209561 (+) 858 WP_018623454.1 pyrroline-5-carboxylate reductase -
  SR900_RS00990 (SR900_00990) - 209584..210171 (+) 588 WP_018623455.1 YggT family protein -
  SR900_RS00995 (SR900_00995) - 210318..210767 (+) 450 WP_018623456.1 DUF4426 domain-containing protein -
  SR900_RS01000 (SR900_01000) rdgB 210882..211514 (+) 633 WP_018623457.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42819.24 Da        Isoelectric Point: 6.6905

>NTDB_id=913153 SR900_RS00970 WP_018623451.1 205671..206810(-) (pilU) [Kangiella aquimarina strain DSMZ 16071]
MDINPLLKALMEHKGSDLFITVHRPPCIKINGQIRPVTKSMLTPAQVEALIHSSMTEKQMKEFEEKKECQYAISVENIGR
FRASAFYQRNQPGLVVRRIETKIPTTEDLKIPNILNELAMKKRGIIIFVGATGTGKSTSLAAMIGYRNRNSAGHIISIED
PIEYVHRHDKCIITQREVGIDTESFETALKNTLRQAPDVILIGEVRSKETMSNAITFAETGHLCLCTLHANNANQAIDRI
LHFFPKEEHSRVLMDLSLNTRAIIAQQLVPTKDGKGRRAAIEILINTPLISDHIRKGEVHLLKELMARSRELGMQTFDQA
LFDLYVEGEISYEDALLYADSANDLRLKIKLHEKDADSLTESTDGLEVEMDDDRSNRMF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=913153 SR900_RS00970 WP_018623451.1 205671..206810(-) (pilU) [Kangiella aquimarina strain DSMZ 16071]
ATGGATATCAATCCACTTTTAAAAGCCTTGATGGAACACAAGGGCTCGGACTTATTTATTACAGTACATCGTCCGCCTTG
TATTAAAATTAACGGACAGATTCGTCCTGTTACCAAAAGCATGCTAACGCCAGCTCAGGTAGAAGCACTAATTCATTCTT
CAATGACCGAAAAGCAGATGAAGGAATTTGAAGAGAAGAAAGAATGCCAATATGCGATCAGTGTTGAGAATATTGGGCGT
TTCCGTGCCAGTGCTTTCTATCAGCGTAACCAGCCAGGTCTGGTTGTTCGTCGAATTGAAACTAAAATCCCGACTACCGA
AGATTTGAAAATCCCTAACATCCTCAATGAACTAGCGATGAAAAAACGTGGGATTATTATTTTCGTAGGTGCAACCGGTA
CCGGTAAATCAACCTCACTGGCAGCCATGATCGGTTATCGAAATCGCAATTCAGCGGGTCACATTATCAGCATTGAGGAT
CCGATTGAGTATGTTCATCGTCATGACAAATGCATCATTACCCAACGTGAGGTTGGGATTGATACAGAATCATTCGAAAC
AGCCCTGAAAAATACCTTACGTCAGGCGCCTGATGTGATTCTGATTGGTGAGGTGCGGAGTAAAGAAACGATGTCGAATG
CGATTACTTTCGCTGAGACGGGTCACTTGTGTTTATGTACCTTGCACGCTAACAACGCGAACCAGGCAATTGACCGTATT
CTGCATTTCTTCCCGAAAGAAGAGCACTCCAGGGTTTTGATGGATTTATCATTGAATACTCGAGCTATTATCGCGCAACA
GCTGGTGCCAACTAAAGATGGTAAAGGTCGTCGTGCAGCTATTGAAATTCTTATTAATACTCCTTTGATTTCAGATCATA
TTCGTAAAGGTGAGGTTCACCTGTTGAAAGAGTTAATGGCTCGCTCCAGAGAGCTGGGCATGCAGACTTTTGACCAGGCA
TTGTTTGATCTCTATGTTGAAGGTGAAATCAGCTATGAAGATGCTCTATTGTATGCTGATTCTGCAAATGACCTACGTCT
GAAAATCAAGCTGCATGAGAAAGATGCAGATTCACTGACAGAAAGTACCGATGGATTGGAGGTTGAGATGGATGATGACC
GAAGCAATCGTATGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.842

100

0.67
  pilU Acinetobacter baylyi ADP1

65.07

93.668

0.609
  pilU Vibrio cholerae strain A1552

55.795

97.889

0.546
  pilT Pseudomonas stutzeri DSM 10701

42.687

88.391

0.377
  pilT Pseudomonas aeruginosa PAK

42.388

88.391

0.375
  pilT Acinetobacter baumannii strain A118

42.09

88.391

0.372
  pilT Acinetobacter baumannii D1279779

42.09

88.391

0.372
  pilT Acinetobacter nosocomialis M2

42.09

88.391

0.372
  pilT Vibrio cholerae strain A1552

42.216

88.127

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

41.791

88.391

0.369
  pilT Legionella pneumophila strain ERS1305867

41.791

88.391

0.369
  pilT Acinetobacter baylyi ADP1

40.762

89.974

0.367