Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SR914_RS09705 Genome accession   NZ_CP140157
Coordinates   2101041..2102084 (-) Length   347 a.a.
NCBI ID   WP_003050584.1    Uniprot ID   A0A076PZN4
Organism   Comamonas testosteroni strain DSMZ 14576     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2096041..2107084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR914_RS09680 (SR914_09680) - 2096890..2097291 (+) 402 WP_003050571.1 YraN family protein -
  SR914_RS09685 (SR914_09685) - 2097381..2097989 (+) 609 WP_003050573.1 SIS domain-containing protein -
  SR914_RS09690 (SR914_09690) - 2097986..2098639 (+) 654 WP_003050576.1 BON domain-containing protein -
  SR914_RS09695 (SR914_09695) - 2098701..2099612 (-) 912 WP_003050579.1 NAD(P)-dependent oxidoreductase -
  SR914_RS09700 (SR914_09700) pilU 2099761..2100897 (-) 1137 WP_003050582.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SR914_RS09705 (SR914_09705) pilT 2101041..2102084 (-) 1044 WP_003050584.1 type IV pilus twitching motility protein PilT Machinery gene
  SR914_RS09710 (SR914_09710) - 2102122..2102853 (+) 732 WP_003050587.1 YggS family pyridoxal phosphate-dependent enzyme -
  SR914_RS09715 (SR914_09715) - 2103019..2104266 (-) 1248 WP_003050590.1 branched-chain amino acid ABC transporter substrate-binding protein -
  SR914_RS09720 (SR914_09720) - 2104313..2105083 (-) 771 WP_019043137.1 TetR/AcrR family transcriptional regulator -
  SR914_RS09725 (SR914_09725) - 2105240..2106838 (+) 1599 WP_003050597.1 AMP-binding protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38370.24 Da        Isoelectric Point: 6.7743

>NTDB_id=913143 SR914_RS09705 WP_003050584.1 2101041..2102084(-) (pilT) [Comamonas testosteroni strain DSMZ 14576]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKTVHAMVYDIMSDAQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFSDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSQNMGMQTLDQN
LTDLVRRNLISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=913143 SR914_RS09705 WP_003050584.1 2101041..2102084(-) (pilT) [Comamonas testosteroni strain DSMZ 14576]
GTGGATATCACGCAATTGCTGGCATTCAGTGTCAAGAACAAGGCTTCGGACTTGCATTTGTCGGCAGGCCTGCCCCCCAT
GATTCGCGTCCACGGCGATGTGCGTCGCATCAACATCGATCCGCTGGACCACAAGACCGTGCACGCCATGGTCTACGACA
TCATGAGCGATGCCCAGCGCAAACAGTACGAAGAGTTTCTGGAAGTGGACTTTTCGTTCGAAATTGAAGGCCTGGCGCGG
TTTCGTGTCAACGCCTTCAACCAGAACCGCGGCGCGGCGGCCGTGTTCCGTACCATTCCCAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCCCCCAAGATCTTCTCCGACCTGGCACTGAAGCCGCGCGGCCTGGTGCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTCAACTATCTCAACGAGACCGAGTACGGCCACATCCTGACGGTGGAAGAC
CCCATCGAATTCGTGCACGAGTCCAAGAAATGCCTGATCAACCAGCGCGAAGTCGGCCCCATGACGCTGTCGTTCGCGGC
AGCCCTGAAATCCGCGCTGCGTGAAGATCCGGACGCGATCCTGGTCGGCGAAATGCGGGACCTGGAAACCATCCGTCTGG
CCATGACGGCGGCCGAAACCGGTCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTCTCCGAGTCGCTGCAGGCAGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGTGTGGCCGCGCACGAGATCATGCTGGGCACGCCTGCCATCCGCAATCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCCAGAACATGGGCATGCAGACCCTGGACCAGAAC
CTGACCGATCTGGTGCGCCGCAACCTCATCAGCCCGGCCGAAGCGCGCAGCAAGGCGAAGATTCCCGAGAACTTCCCTGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A076PZN4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.742

98.271

0.666
  pilT Vibrio cholerae strain A1552

67.742

98.271

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

44.478

96.542

0.429
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409