Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SR914_RS09700 Genome accession   NZ_CP140157
Coordinates   2099761..2100897 (-) Length   378 a.a.
NCBI ID   WP_003050582.1    Uniprot ID   B7WR82
Organism   Comamonas testosteroni strain DSMZ 14576     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2094761..2105897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR914_RS09675 (SR914_09675) rsmI 2095967..2096890 (-) 924 WP_019043139.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  SR914_RS09680 (SR914_09680) - 2096890..2097291 (+) 402 WP_003050571.1 YraN family protein -
  SR914_RS09685 (SR914_09685) - 2097381..2097989 (+) 609 WP_003050573.1 SIS domain-containing protein -
  SR914_RS09690 (SR914_09690) - 2097986..2098639 (+) 654 WP_003050576.1 BON domain-containing protein -
  SR914_RS09695 (SR914_09695) - 2098701..2099612 (-) 912 WP_003050579.1 NAD(P)-dependent oxidoreductase -
  SR914_RS09700 (SR914_09700) pilU 2099761..2100897 (-) 1137 WP_003050582.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SR914_RS09705 (SR914_09705) pilT 2101041..2102084 (-) 1044 WP_003050584.1 type IV pilus twitching motility protein PilT Machinery gene
  SR914_RS09710 (SR914_09710) - 2102122..2102853 (+) 732 WP_003050587.1 YggS family pyridoxal phosphate-dependent enzyme -
  SR914_RS09715 (SR914_09715) - 2103019..2104266 (-) 1248 WP_003050590.1 branched-chain amino acid ABC transporter substrate-binding protein -
  SR914_RS09720 (SR914_09720) - 2104313..2105083 (-) 771 WP_019043137.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42021.53 Da        Isoelectric Point: 6.7687

>NTDB_id=913142 SR914_RS09700 WP_003050582.1 2099761..2100897(-) (pilU) [Comamonas testosteroni strain DSMZ 14576]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGKVTKVSPQPLSQLHTLALARSIMNDKQVADFEHTKECNFAI
SPAGVGRFRVNAFMQQGKVGMVLRTIPTTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTESWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDVSLNLRAIISQRLIPKQDSKGRVAAIEVMLNSPLIADLIFKGEVVEIKEIMKKSRNIG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLNSQRARTLDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=913142 SR914_RS09700 WP_003050582.1 2099761..2100897(-) (pilU) [Comamonas testosteroni strain DSMZ 14576]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCGGAGTTCCCGCCCGCCATCAAGATTGACGGCAAGGTCACCAAGGTCTCGCCACAGCCGCTGAGCCAGCTGCATA
CGCTGGCCCTGGCGCGCTCCATCATGAACGACAAGCAGGTGGCGGACTTCGAGCACACCAAGGAGTGCAACTTCGCCATC
TCGCCGGCCGGCGTGGGGCGCTTCCGCGTCAATGCCTTCATGCAGCAGGGCAAGGTCGGCATGGTGCTGCGTACCATTCC
CACCACCCTGCCCACGATTGACGGCCTGGGCGTGCCACAGGTGCTCAAGGAGGTCACCATGACCAAGCGCGGCCTGTGCA
TTCTGGTCGGCGCGACGGGCTCCGGCAAATCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCCGTGGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAACGCGAGGTGGGACT
GGATACCGAAAGCTGGGAAGCGGCGCTCAAGAATACGCTGCGCCAGGCTCCCGATGTGATCTTGATGGGCGAAATCCGCG
ACCGTGAAACCATGGAGCACGCGATTGCGTTCTCGGAAACCGGTCACCTGTGCCTGGCCACGCTGCATGCCAACAGTGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTTCCCGAAGAACGCCGTCCACAGCTGCTGATGGATGTGTCGCTGAACCT
GCGGGCCATCATCTCCCAGCGTCTGATTCCCAAGCAGGACAGCAAGGGCCGTGTCGCCGCCATCGAGGTGATGCTCAACT
CTCCGCTGATTGCCGACCTGATCTTCAAGGGCGAGGTCGTCGAGATCAAGGAGATCATGAAGAAAAGCCGCAATATCGGC
ATGCAGACCTTCGATCAGGCGCTGTTCGACCTGTTCGAATCCAATCTCATCACCTACGAAGATGCGCTGCGCAATGCAGA
CTCGGTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCCAGCGCGCCCGGACTCTGGACCTGGCAGCCGGCACCGAGC
ATCTGACCATCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7WR82

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.202

96.825

0.593
  pilU Acinetobacter baylyi ADP1

56.267

94.974

0.534
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

42.342

88.095

0.373
  pilT Legionella pneumophila strain Lp02

42.342

88.095

0.373
  pilT Acinetobacter baylyi ADP1

42.073

86.772

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362