Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SR882_RS10005 Genome accession   NZ_CP140153
Coordinates   2210249..2211298 (+) Length   349 a.a.
NCBI ID   WP_322521102.1    Uniprot ID   -
Organism   Guyparkeria halophila strain ATCC 49870     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2205249..2216298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR882_RS09975 (SR882_09975) - 2205431..2206030 (+) 600 WP_322521096.1 methyltransferase -
  SR882_RS09980 (SR882_09980) metW 2206091..2206696 (-) 606 WP_322521097.1 methionine biosynthesis protein MetW -
  SR882_RS09985 (SR882_09985) - 2206693..2207901 (-) 1209 WP_322521098.1 homoserine O-acetyltransferase -
  SR882_RS09990 (SR882_09990) - 2207928..2208479 (-) 552 WP_322521099.1 YggT family protein -
  SR882_RS09995 (SR882_09995) proC 2208540..2209379 (-) 840 WP_322521100.1 pyrroline-5-carboxylate reductase -
  SR882_RS10000 (SR882_10000) - 2209455..2210117 (-) 663 WP_322521101.1 YggS family pyridoxal phosphate-dependent enzyme -
  SR882_RS10005 (SR882_10005) pilT 2210249..2211298 (+) 1050 WP_322521102.1 type IV pilus twitching motility protein PilT Machinery gene
  SR882_RS10010 (SR882_10010) pilU 2211445..2212590 (+) 1146 WP_322521103.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SR882_RS10015 (SR882_10015) - 2212623..2213777 (+) 1155 WP_322521104.1 PilT/PilU family type 4a pilus ATPase -
  SR882_RS10025 (SR882_10025) thiO 2214047..2215123 (+) 1077 WP_322521105.1 glycine oxidase ThiO -
  SR882_RS10030 (SR882_10030) - 2215151..2215489 (-) 339 WP_125199332.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 39082.51 Da        Isoelectric Point: 7.1150

>NTDB_id=913106 SR882_RS10005 WP_322521102.1 2210249..2211298(+) (pilT) [Guyparkeria halophila strain ATCC 49870]
MAESRDIDSLLMFTVKNGASDLHLSAGEQPRVRIDGDIRKVNVPVMEHKDVHTMVYDIMNDRQRKEYEDNLEVDFSFELR
GHARFRVNAFNQNRGASAVFRTIPSKVLTLEQLDTPKVFQDLCDYPNGIVLVTGPTGSGKSTTLAAMVDYINDHRNDHIL
TVEDPIEFVHDSKKCLVNQREVHRDTHSFNAALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTNSAAKT
IDRIIDVFPSAEKDMVRAMLSESLRAVISQTLLKKISGGRVAAHEIMLGTPAIRNLIRENKVAQMYSAIQTGQAQGMQTL
DQNLKQLVTRGVVSKELARGRAKNKDDFR

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=913106 SR882_RS10005 WP_322521102.1 2210249..2211298(+) (pilT) [Guyparkeria halophila strain ATCC 49870]
ATGGCCGAATCCCGAGATATCGACTCCCTGCTGATGTTCACCGTCAAGAACGGGGCATCGGACCTCCACCTGTCGGCCGG
CGAACAGCCCCGCGTGCGCATCGACGGCGACATCCGCAAGGTCAACGTGCCGGTGATGGAGCACAAGGATGTCCACACCA
TGGTGTACGACATCATGAACGACCGGCAGCGCAAGGAGTACGAGGACAACCTCGAGGTCGACTTCTCCTTCGAACTGCGC
GGCCATGCCCGCTTCCGTGTCAACGCCTTCAACCAGAACCGCGGCGCGAGTGCGGTCTTCCGCACCATCCCGAGCAAGGT
TCTGACCCTCGAGCAACTCGACACCCCCAAGGTGTTCCAGGATCTGTGCGACTACCCCAACGGCATCGTGCTGGTCACCG
GCCCGACCGGCTCGGGCAAGTCGACCACGCTCGCGGCGATGGTCGACTACATCAATGATCACCGCAACGACCATATCCTG
ACCGTCGAGGATCCGATCGAATTCGTCCACGACTCGAAAAAGTGCCTGGTCAACCAGCGCGAGGTCCATCGCGACACGCA
CAGCTTCAACGCCGCGTTGCGCTCGGCGCTGCGTGAGGACCCGGACGTGGTCCTGGTCGGCGAATTGCGGGACCTCGAGA
CCATCCGTCTGGCGCTGACCGCCGCGGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCAACTCGGCGGCCAAGACC
ATCGACCGGATCATCGACGTCTTCCCCTCGGCGGAGAAGGACATGGTGCGCGCGATGCTCTCCGAGTCCCTGCGCGCGGT
CATCTCACAGACCCTGCTCAAGAAGATCAGCGGTGGCCGGGTCGCGGCGCACGAGATCATGCTGGGCACGCCGGCGATCC
GCAACCTGATCCGCGAGAACAAGGTCGCGCAGATGTACTCCGCCATCCAGACCGGTCAGGCACAAGGGATGCAGACGCTC
GACCAGAATCTCAAGCAGCTGGTCACCCGTGGCGTCGTCTCCAAGGAACTGGCCCGCGGCCGGGCGAAGAACAAGGACGA
CTTCAGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.843

98.281

0.765
  pilT Acinetobacter baumannii D1279779

77.843

98.281

0.765
  pilT Acinetobacter baumannii strain A118

77.843

98.281

0.765
  pilT Acinetobacter baylyi ADP1

74.344

98.281

0.731
  pilT Pseudomonas aeruginosa PAK

73.469

98.281

0.722
  pilT Pseudomonas stutzeri DSM 10701

72.303

98.281

0.711
  pilT Legionella pneumophila strain Lp02

71.72

98.281

0.705
  pilT Legionella pneumophila strain ERS1305867

71.72

98.281

0.705
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.39

93.983

0.699
  pilT Vibrio cholerae strain A1552

74.39

93.983

0.699
  pilT Neisseria meningitidis 8013

65.889

98.281

0.648
  pilT Neisseria gonorrhoeae MS11

65.598

98.281

0.645
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.711

100

0.487
  pilU Pseudomonas stutzeri DSM 10701

40.87

98.854

0.404
  pilU Vibrio cholerae strain A1552

42.169

95.129

0.401
  pilU Acinetobacter baylyi ADP1

37.861

99.14

0.375