Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SR882_RS10010 Genome accession   NZ_CP140153
Coordinates   2211445..2212590 (+) Length   381 a.a.
NCBI ID   WP_322521103.1    Uniprot ID   -
Organism   Guyparkeria halophila strain ATCC 49870     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2206445..2217590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR882_RS09985 (SR882_09985) - 2206693..2207901 (-) 1209 WP_322521098.1 homoserine O-acetyltransferase -
  SR882_RS09990 (SR882_09990) - 2207928..2208479 (-) 552 WP_322521099.1 YggT family protein -
  SR882_RS09995 (SR882_09995) proC 2208540..2209379 (-) 840 WP_322521100.1 pyrroline-5-carboxylate reductase -
  SR882_RS10000 (SR882_10000) - 2209455..2210117 (-) 663 WP_322521101.1 YggS family pyridoxal phosphate-dependent enzyme -
  SR882_RS10005 (SR882_10005) pilT 2210249..2211298 (+) 1050 WP_322521102.1 type IV pilus twitching motility protein PilT Machinery gene
  SR882_RS10010 (SR882_10010) pilU 2211445..2212590 (+) 1146 WP_322521103.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SR882_RS10015 (SR882_10015) - 2212623..2213777 (+) 1155 WP_322521104.1 PilT/PilU family type 4a pilus ATPase -
  SR882_RS10025 (SR882_10025) thiO 2214047..2215123 (+) 1077 WP_322521105.1 glycine oxidase ThiO -
  SR882_RS10030 (SR882_10030) - 2215151..2215489 (-) 339 WP_125199332.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42521.44 Da        Isoelectric Point: 5.4803

>NTDB_id=913107 SR882_RS10010 WP_322521103.1 2211445..2212590(+) (pilU) [Guyparkeria halophila strain ATCC 49870]
MDRQQAANYVQALLKRAVERDASDLFVTVGAPPSAKIDGKVVHLTDQKLSVENTQMLVRSIMTDKQVAHFEESHEANFAI
ALPGVARFRVNAFMQRGSEGMVLRMIRSDIPNFDDLKLPDVLGEIAMTARGLVIFVGATSSGKSTSLAAMLDYRNENSRG
HIVTIEDPIEFVHKHKGCLVTQREVGVDTDSFAAALKNTLRQAPDVILIGEIRDRETMDHAIAFAETGHLCLATLHANNT
NQALDRIINFFPEERRQQLLMDLSLNLKSVISQRLLRKADGEGRVPAVEVLINTPLIGDLVFKGDVSEIKEVMKRSRELG
MQTFDQALFDLYDSHQADYDEVISKADSVNDLRLRIKLESKRGLPKSRQDDSEDDLKVEAD

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=913107 SR882_RS10010 WP_322521103.1 2211445..2212590(+) (pilU) [Guyparkeria halophila strain ATCC 49870]
ATGGACCGTCAGCAAGCAGCCAACTACGTACAGGCCCTTCTCAAGCGGGCCGTCGAACGCGACGCCTCCGACCTGTTCGT
CACCGTCGGCGCGCCGCCGTCGGCCAAGATCGACGGCAAGGTCGTCCACCTGACCGACCAGAAACTCTCGGTGGAAAACA
CCCAGATGCTGGTCCGCTCGATCATGACGGACAAGCAGGTGGCCCATTTCGAGGAATCGCACGAAGCGAACTTCGCGATC
GCCCTGCCCGGCGTGGCGCGCTTTCGCGTCAACGCGTTCATGCAGCGCGGCAGCGAGGGCATGGTCCTGCGGATGATCCG
TTCGGACATACCCAACTTCGACGATCTCAAGCTTCCCGACGTGCTGGGCGAAATCGCCATGACCGCCCGGGGCCTGGTGA
TCTTCGTCGGCGCCACCAGTTCGGGCAAGTCGACCTCGTTGGCGGCGATGCTCGACTACCGCAACGAGAACTCGCGCGGG
CATATCGTCACCATCGAGGATCCGATCGAGTTCGTCCACAAGCACAAGGGCTGCCTGGTCACCCAGCGCGAGGTGGGCGT
CGACACCGACTCCTTCGCCGCGGCGCTGAAGAACACCCTGCGCCAGGCCCCCGACGTGATCCTGATCGGCGAGATCCGCG
ATCGCGAGACCATGGATCACGCCATCGCCTTTGCCGAGACCGGTCACCTCTGTCTGGCCACGCTCCACGCGAACAACACC
AACCAGGCACTCGACCGCATCATCAACTTCTTCCCCGAGGAACGGCGACAGCAGTTGCTGATGGACCTGTCGCTCAACTT
GAAGTCGGTGATCTCGCAGCGACTGCTGCGCAAGGCCGATGGCGAGGGCCGTGTCCCGGCCGTCGAGGTGCTGATCAACA
CGCCCCTGATCGGCGACCTGGTCTTCAAGGGCGACGTCTCCGAGATCAAGGAGGTCATGAAGCGCTCGCGCGAACTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGACCTCTATGACAGCCACCAGGCCGATTACGACGAGGTCATCAGCAAGGCCGA
CTCGGTCAACGACCTGCGCCTGCGCATCAAGCTCGAGAGCAAGCGCGGCCTGCCCAAGAGCCGCCAGGACGATTCCGAAG
ACGATCTCAAGGTCGAGGCCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.393

92.126

0.575
  pilU Acinetobacter baylyi ADP1

57.951

97.375

0.564
  pilU Vibrio cholerae strain A1552

55.848

89.764

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.611

87.664

0.391
  pilT Pseudomonas stutzeri DSM 10701

45.141

83.727

0.378
  pilT Acinetobacter nosocomialis M2

44.72

84.514

0.378
  pilT Acinetobacter baumannii D1279779

44.72

84.514

0.378
  pilT Acinetobacter baumannii strain A118

44.72

84.514

0.378
  pilT Pseudomonas aeruginosa PAK

44.828

83.727

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.012

88.714

0.373
  pilT Vibrio cholerae strain A1552

42.012

88.714

0.373
  pilT Acinetobacter baylyi ADP1

43.478

84.514

0.367