Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SR858_RS02705 Genome accession   NZ_CP140152
Coordinates   603255..604298 (-) Length   347 a.a.
NCBI ID   WP_019924644.1    Uniprot ID   A0A6I1MYL1
Organism   Duganella zoogloeoides strain ATCC 25935     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 598255..609298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR858_RS02690 (SR858_02690) - 598913..601447 (-) 2535 WP_019924647.1 EAL domain-containing protein -
  SR858_RS02695 (SR858_02695) - 601617..602105 (-) 489 WP_019924646.1 glutathione peroxidase -
  SR858_RS02700 (SR858_02700) pilU 602116..603252 (-) 1137 WP_026637801.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SR858_RS02705 (SR858_02705) pilT 603255..604298 (-) 1044 WP_019924644.1 type IV pilus twitching motility protein PilT Machinery gene
  SR858_RS02710 (SR858_02710) - 604349..605050 (+) 702 WP_019924643.1 YggS family pyridoxal phosphate-dependent enzyme -
  SR858_RS02715 (SR858_02715) proC 605052..605879 (+) 828 WP_019924642.1 pyrroline-5-carboxylate reductase -
  SR858_RS02720 (SR858_02720) - 606066..606416 (-) 351 WP_051120404.1 YqjD family protein -
  SR858_RS02725 (SR858_02725) ubiA 606499..607353 (-) 855 WP_019924640.1 4-hydroxybenzoate octaprenyltransferase -
  SR858_RS02730 (SR858_02730) - 607414..608349 (-) 936 WP_019924639.1 hydrogen peroxide-inducible genes activator -
  SR858_RS02735 (SR858_02735) rfaE2 608467..608952 (+) 486 WP_026637799.1 D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase -
  SR858_RS02740 (SR858_02740) - 608949..609236 (+) 288 WP_019924637.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38264.80 Da        Isoelectric Point: 6.8286

>NTDB_id=913076 SR858_RS02705 WP_019924644.1 603255..604298(-) (pilT) [Duganella zoogloeoides strain ATCC 25935]
MDISELLAFSVKNKASDLHLSAGLPPMLRVHGDVRRLNVDALEHKEVHRMIYDIMNDGQRKVYEERLEIDFSFEIPGLSR
FRVNAFNQERGASAVFRTIPSKILTLEELNAPKIFADFALKPRGLVLVTGPTGSGKSTTLAAMVNHLNETEHGHILTIED
PIEFVHDSKKCLINQREVGPHTHSFSNALRSALREDPDCILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMVGTPAIRNLIREAKIAQMYSAIQTGSNFGMQTLDQN
LTDLVRRNVIASATARSAAKTPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=913076 SR858_RS02705 WP_019924644.1 603255..604298(-) (pilT) [Duganella zoogloeoides strain ATCC 25935]
ATGGACATTTCCGAACTTCTCGCATTCTCCGTCAAGAACAAGGCCTCGGATCTGCACCTTTCGGCCGGGCTGCCGCCGAT
GCTGCGCGTGCACGGCGACGTGCGGCGCCTGAACGTCGATGCGCTCGAGCACAAGGAAGTGCACCGCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGGTCTATGAAGAACGCCTGGAGATCGACTTTTCGTTCGAGATTCCCGGCCTGTCGCGT
TTTCGCGTCAATGCCTTCAACCAGGAGCGCGGCGCCTCGGCGGTGTTCCGCACCATCCCGTCGAAAATCCTCACGCTCGA
AGAGCTGAACGCGCCCAAGATCTTTGCCGATTTCGCCTTGAAACCGCGCGGCCTGGTGCTGGTGACCGGCCCCACCGGTT
CGGGCAAATCGACCACGCTGGCGGCCATGGTCAACCATCTCAACGAGACCGAGCATGGCCACATCCTGACGATCGAAGAC
CCGATCGAATTCGTGCACGACTCGAAAAAATGCCTGATCAACCAGCGCGAGGTGGGACCGCACACGCATTCGTTCAGTAA
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACTGCATCCTGGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGTCGGCCGCCGAAACGGGCCACCTGGTGTTCGGCACCCTGCACACATCGTCGGCCGCCAAGACCATCGACCGTATC
GTCGATGTATTCCCGGCCGAGGAAAAGGAAATGGTGCGGGCCATGCTGTCGGAGTCCCTGCAGGCGGTGATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGCAGCGGGCGCGTGGCTGCGCATGAAATCATGGTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCGAAGATCGCGCAGATGTATTCGGCGATCCAGACCGGCAGCAACTTCGGCATGCAGACGCTGGACCAGAAC
CTGACGGACCTGGTGCGCCGCAACGTGATCGCGTCGGCCACCGCGCGCAGCGCGGCCAAGACCCCCGACAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I1MYL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

72.566

97.695

0.709
  pilT Legionella pneumophila strain ERS1305867

72.566

97.695

0.709
  pilT Vibrio cholerae strain A1552

70.623

97.118

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.623

97.118

0.686
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Vibrio cholerae strain A1552

40

99.424

0.398
  pilU Pseudomonas stutzeri DSM 10701

41.358

93.372

0.386