Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SR858_RS02700 Genome accession   NZ_CP140152
Coordinates   602116..603252 (-) Length   378 a.a.
NCBI ID   WP_026637801.1    Uniprot ID   -
Organism   Duganella zoogloeoides strain ATCC 25935     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 597116..608252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR858_RS02685 (SR858_02685) glcF 597689..598921 (+) 1233 WP_019924648.1 glycolate oxidase subunit GlcF -
  SR858_RS02690 (SR858_02690) - 598913..601447 (-) 2535 WP_019924647.1 EAL domain-containing protein -
  SR858_RS02695 (SR858_02695) - 601617..602105 (-) 489 WP_019924646.1 glutathione peroxidase -
  SR858_RS02700 (SR858_02700) pilU 602116..603252 (-) 1137 WP_026637801.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SR858_RS02705 (SR858_02705) pilT 603255..604298 (-) 1044 WP_019924644.1 type IV pilus twitching motility protein PilT Machinery gene
  SR858_RS02710 (SR858_02710) - 604349..605050 (+) 702 WP_019924643.1 YggS family pyridoxal phosphate-dependent enzyme -
  SR858_RS02715 (SR858_02715) proC 605052..605879 (+) 828 WP_019924642.1 pyrroline-5-carboxylate reductase -
  SR858_RS02720 (SR858_02720) - 606066..606416 (-) 351 WP_051120404.1 YqjD family protein -
  SR858_RS02725 (SR858_02725) ubiA 606499..607353 (-) 855 WP_019924640.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41723.19 Da        Isoelectric Point: 6.5935

>NTDB_id=913075 SR858_RS02700 WP_026637801.1 602116..603252(-) (pilU) [Duganella zoogloeoides strain ATCC 25935]
MERDNASKFMFDLLRNMLARKGSDLFITAGFPPAMKIDGKMTPMSQQALTPAHTLDLARAIMNDKQAAGFEQTKEANFAI
SPPDLGRFRVSAFVQMGSVGMVLRVITSTIPDLNNLGLPEVLKEVVMAKRGLVIMVGATGSGKSTTLAAMVGYRNENSYG
HIITIEDPVEFVHPHKNCIMTQREVGVDTDDWETALKNTLRQAPDVIQIGEIRDRETMDHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGLVSQRLVPRKGAPGRAVAIEILLNTPLISDLIFKGQVHDIKELMKKSRELG
MQTFDQSLFDLHEAGLITYEDALRNADSVNDLRLAIKLESKTTPEQAPPAGARQLGIL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=913075 SR858_RS02700 WP_026637801.1 602116..603252(-) (pilU) [Duganella zoogloeoides strain ATCC 25935]
ATGGAACGCGACAACGCTTCAAAATTCATGTTCGACCTGCTGCGCAATATGCTTGCGAGAAAAGGGTCGGACCTGTTCAT
CACCGCCGGTTTTCCGCCCGCGATGAAAATCGACGGCAAGATGACCCCGATGTCGCAGCAGGCGCTCACGCCCGCGCACA
CGCTGGACCTGGCGCGCGCCATCATGAACGACAAGCAGGCCGCCGGGTTCGAGCAGACCAAGGAAGCCAACTTCGCCATC
AGTCCGCCCGACCTGGGACGCTTCCGCGTCTCGGCGTTCGTGCAAATGGGCTCGGTGGGCATGGTGCTGCGGGTGATCAC
CAGCACCATCCCCGACCTGAACAACCTGGGCCTGCCGGAAGTGCTGAAGGAAGTGGTGATGGCCAAGCGCGGCCTGGTGA
TCATGGTGGGCGCCACCGGTTCCGGCAAATCGACCACCCTGGCGGCCATGGTCGGGTACCGCAACGAGAACAGCTACGGC
CATATCATCACCATCGAAGACCCGGTGGAATTCGTCCACCCGCACAAGAACTGCATCATGACCCAGCGCGAAGTGGGGGT
CGATACCGACGACTGGGAGACGGCGCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCGAAACCATGGACCACGCGATCGCGTTTGCGGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAACGCCGCCAACAGCTGCTGATGGATTTGTCGCTCAACCT
CAAGGGACTGGTTTCGCAGCGACTGGTGCCGCGCAAGGGCGCGCCGGGGCGCGCGGTGGCAATCGAAATCCTGCTCAACA
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCCAGGTACACGACATCAAGGAGTTGATGAAAAAATCGCGCGAGCTCGGC
ATGCAAACCTTCGACCAGTCGCTGTTCGACCTGCACGAGGCGGGCCTGATCACGTACGAGGACGCGCTGCGCAATGCCGA
TTCGGTCAACGACCTGCGCCTGGCCATCAAGCTCGAAAGCAAAACCACGCCGGAGCAGGCGCCGCCTGCCGGCGCCAGAC
AACTGGGCATCCTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.559

93.651

0.595
  pilU Acinetobacter baylyi ADP1

58.621

92.063

0.54
  pilU Vibrio cholerae strain A1552

56.977

91.005

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.624

0.386
  pilT Pseudomonas aeruginosa PAK

43.465

87.037

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.249

87.037

0.368
  pilT Acinetobacter nosocomialis M2

41.692

87.566

0.365
  pilT Acinetobacter baumannii D1279779

41.692

87.566

0.365
  pilT Acinetobacter baumannii strain A118

41.692

87.566

0.365
  pilT Acinetobacter baylyi ADP1

42.025

86.243

0.362