Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SR908_RS09180 Genome accession   NZ_CP140151
Coordinates   1926206..1927246 (+) Length   346 a.a.
NCBI ID   WP_097021298.1    Uniprot ID   A0A285V9W5
Organism   Chromohalobacter canadensis strain ATCC 43984     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1921206..1932246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR908_RS09150 (SR908_09150) sfsA 1921395..1922141 (+) 747 WP_097021303.1 DNA/RNA nuclease SfsA -
  SR908_RS09155 (SR908_09155) metW 1922143..1922742 (-) 600 WP_097021302.1 methionine biosynthesis protein MetW -
  SR908_RS09160 (SR908_09160) - 1922742..1923902 (-) 1161 WP_040239874.1 homoserine O-acetyltransferase -
  SR908_RS09165 (SR908_09165) - 1923963..1924553 (-) 591 WP_246924029.1 YggT family protein -
  SR908_RS09170 (SR908_09170) proC 1924590..1925414 (-) 825 WP_097021300.1 pyrroline-5-carboxylate reductase -
  SR908_RS09175 (SR908_09175) - 1925464..1926171 (-) 708 WP_097021299.1 YggS family pyridoxal phosphate-dependent enzyme -
  SR908_RS09180 (SR908_09180) pilT 1926206..1927246 (+) 1041 WP_097021298.1 type IV pilus twitching motility protein PilT Machinery gene
  SR908_RS09185 (SR908_09185) pilU 1927284..1928426 (+) 1143 WP_246924026.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SR908_RS09190 (SR908_09190) - 1928431..1929546 (-) 1116 WP_246924023.1 anhydro-N-acetylmuramic acid kinase -
  SR908_RS09195 (SR908_09195) tyrS 1929646..1930842 (+) 1197 WP_246924019.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38147.65 Da        Isoelectric Point: 6.7820

>NTDB_id=913063 SR908_RS09180 WP_097021298.1 1926206..1927246(+) (pilT) [Chromohalobacter canadensis strain ATCC 43984]
MDIIELLAFSAKQKASDLHLSAGLPPMIRVDGDIRRLNVPPIEDRDVRKLIYAIMNDQQRRDYEEALEHDFSFEVPGVAR
FRVNVFHQGRGAGAVFRTIPSEVLTLDDLDLGDVFRRLASLPRGLVLVTGPTGSGKSTTLAAMIDYVNENYYQHVLTIED
PVEFVHRSKRSLVNQRELHRDTRSFATALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRI
IDVFPGDEKSMVRSMLSESLQGVISQVLLKREGGGRVAAHEILVASAAVRNLIRENKLAQMYSSIQTGGSLGMQTLDAAL
SRLVAQDVVSRAEAQAKAKDGRVLGM

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=913063 SR908_RS09180 WP_097021298.1 1926206..1927246(+) (pilT) [Chromohalobacter canadensis strain ATCC 43984]
ATGGATATTATCGAATTGCTGGCGTTCTCGGCAAAACAGAAGGCGTCTGATCTGCATCTGTCCGCCGGTTTGCCGCCAAT
GATTCGCGTCGACGGCGATATTCGCCGGCTCAACGTGCCGCCGATCGAGGATCGTGACGTTCGCAAGTTGATCTACGCGA
TCATGAACGATCAACAGCGCCGCGACTATGAAGAGGCGCTCGAGCATGATTTCTCGTTCGAGGTGCCGGGGGTGGCGCGT
TTCCGTGTCAACGTCTTCCACCAGGGGCGCGGCGCGGGCGCTGTCTTCCGTACCATTCCCAGCGAAGTTCTCACCCTGGA
TGACCTGGACCTGGGCGATGTGTTTCGACGCCTGGCCTCGCTGCCGCGCGGGTTGGTGCTGGTGACGGGGCCTACCGGGT
CGGGCAAGAGTACGACCTTGGCGGCGATGATCGACTACGTCAACGAAAACTACTATCAACATGTGCTGACCATCGAGGAT
CCAGTAGAGTTCGTGCATCGCAGCAAACGCAGTCTCGTCAATCAGCGCGAATTGCATCGCGATACGCGCAGCTTCGCCAC
GGCGTTGCGTTCGGCGCTGCGCGAGGACCCCGACGTGATTCTGGTCGGCGAGTTGCGCGACCTGGAGACCATCCGTCTGG
CGCTGACCGCCGCCGAAACCGGGCACCTGGTGTTCGGTACCTTGCATACCACCTCGGCGGCCAAGACTGTGGATCGCATC
ATCGATGTGTTCCCGGGCGACGAGAAAAGCATGGTGCGTTCGATGTTGTCCGAGTCGTTGCAAGGGGTGATCTCGCAGGT
CCTGCTCAAGCGTGAGGGTGGCGGGCGCGTGGCGGCCCACGAGATCCTGGTGGCCAGCGCGGCGGTGCGTAACTTGATAC
GCGAGAACAAGCTGGCGCAGATGTATTCCTCGATCCAGACCGGCGGCAGCCTAGGCATGCAGACACTGGATGCGGCTCTT
TCGCGCTTGGTGGCGCAGGATGTCGTCTCGCGCGCCGAGGCGCAGGCCAAGGCGAAGGATGGGCGCGTGCTGGGCATGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A285V9W5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.811

97.977

0.743
  pilT Pseudomonas stutzeri DSM 10701

75.811

97.977

0.743
  pilT Acinetobacter baumannii D1279779

73.451

97.977

0.72
  pilT Acinetobacter baumannii strain A118

73.451

97.977

0.72
  pilT Acinetobacter nosocomialis M2

73.156

97.977

0.717
  pilT Acinetobacter baylyi ADP1

72.566

97.977

0.711
  pilT Legionella pneumophila strain Lp02

69.822

97.688

0.682
  pilT Legionella pneumophila strain ERS1305867

69.822

97.688

0.682
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.399

0.662
  pilT Vibrio cholerae strain A1552

67.953

97.399

0.662
  pilT Neisseria meningitidis 8013

66.276

98.555

0.653
  pilT Neisseria gonorrhoeae MS11

65.982

98.555

0.65
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.32

98.555

0.506
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.821

0.402
  pilU Vibrio cholerae strain A1552

41.194

96.821

0.399
  pilU Acinetobacter baylyi ADP1

39.118

98.266

0.384