Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U0024_RS24105 Genome accession   NZ_CP139994
Coordinates   5277545..5278588 (+) Length   347 a.a.
NCBI ID   WP_302088810.1    Uniprot ID   -
Organism   Comamonas testosteroni strain HAMBI_0403     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5272545..5283588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0024_RS24085 (U0024_24090) - 5272714..5274312 (-) 1599 WP_302088814.1 AMP-binding protein -
  U0024_RS24090 (U0024_24095) - 5274565..5275359 (+) 795 WP_302088813.1 TetR/AcrR family transcriptional regulator -
  U0024_RS24095 (U0024_24100) - 5275407..5276654 (+) 1248 WP_304351789.1 branched-chain amino acid ABC transporter substrate-binding protein -
  U0024_RS24100 (U0024_24105) - 5276785..5277507 (-) 723 WP_304351790.1 YggS family pyridoxal phosphate-dependent enzyme -
  U0024_RS24105 (U0024_24110) pilT 5277545..5278588 (+) 1044 WP_302088810.1 type IV pilus twitching motility protein PilT Machinery gene
  U0024_RS24110 (U0024_24115) pilU 5278712..5279848 (+) 1137 WP_302088809.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U0024_RS24115 (U0024_24120) - 5280008..5280919 (+) 912 WP_304351791.1 NAD(P)-dependent oxidoreductase -
  U0024_RS24120 (U0024_24125) - 5280981..5281634 (-) 654 WP_302088807.1 BON domain-containing protein -
  U0024_RS24125 (U0024_24130) - 5281631..5282239 (-) 609 WP_043375490.1 SIS domain-containing protein -
  U0024_RS24130 (U0024_24135) - 5282329..5282730 (-) 402 WP_302088806.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38370.24 Da        Isoelectric Point: 6.7745

>NTDB_id=912307 U0024_RS24105 WP_302088810.1 5277545..5278588(+) (pilT) [Comamonas testosteroni strain HAMBI_0403]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKTVHAMVYDIMSDAQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRSMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSQNMGMQTLDQN
LTDLVRRNLISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=912307 U0024_RS24105 WP_302088810.1 5277545..5278588(+) (pilT) [Comamonas testosteroni strain HAMBI_0403]
GTGGATATCACGCAATTGCTGGCATTCAGTGTCAAGAACAAGGCTTCGGACTTGCATTTGTCGGCAGGCCTGCCCCCCAT
GATTCGCGTCCACGGCGATGTGCGTCGCATCAACATCGATCCGCTGGACCACAAGACCGTGCACGCCATGGTCTACGACA
TCATGAGCGATGCCCAGCGCAAGCAGTACGAAGAGTTTCTGGAAGTGGACTTTTCGTTCGAGATCGAAGGCCTGGCCCGT
TTTCGTGTCAATGCCTTCAACCAGAACCGCGGCGCGGCGGCCGTGTTCCGTACCATTCCCAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCCCCCAAGATCTTTGGCGAGCTGGCCCTGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTCAACTACCTCAACGAGACCGAGTACGGCCATATCCTGACGGTGGAAGAC
CCCATCGAATTCGTGCACGAGTCCAAGAAATGCCTGATCAACCAGCGCGAAGTCGGCCCCATGACGCTGTCGTTCGCGGC
AGCGCTGAAGTCCGCGCTGCGTGAAGACCCGGACGCGATTCTGGTTGGCGAAATGCGAGACCTGGAAACCATCCGCCTGG
CCATGACGGCGGCCGAAACCGGTCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTGTTTCCGGCCGAGGAAAAGGAAATGGTGCGCTCCATGCTCTCCGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGTGTGGCCGCGCACGAGATCATGCTGGGCACGCCCGCCATCCGCAACCTGA
TTCGCGAGGCCAAGGTGGCACAGATGTACTCCACCATCCAGACCAGCCAGAACATGGGCATGCAGACCCTGGACCAGAAC
CTGACCGATCTGGTGCGCCGCAACCTCATCAGCCCGGCCGAAGCGCGCAGCAAGGCCAAGATTCCCGAGAACTTTCCGGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

74.631

97.695

0.729
  pilT Legionella pneumophila strain ERS1305867

74.631

97.695

0.729
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Neisseria meningitidis 8013

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.742

98.271

0.666
  pilT Vibrio cholerae strain A1552

67.742

98.271

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

44.478

96.542

0.429
  pilU Acinetobacter baylyi ADP1

42.197

99.712

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409