Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U0024_RS24110 Genome accession   NZ_CP139994
Coordinates   5278712..5279848 (+) Length   378 a.a.
NCBI ID   WP_302088809.1    Uniprot ID   -
Organism   Comamonas testosteroni strain HAMBI_0403     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5273712..5284848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0024_RS24090 (U0024_24095) - 5274565..5275359 (+) 795 WP_302088813.1 TetR/AcrR family transcriptional regulator -
  U0024_RS24095 (U0024_24100) - 5275407..5276654 (+) 1248 WP_304351789.1 branched-chain amino acid ABC transporter substrate-binding protein -
  U0024_RS24100 (U0024_24105) - 5276785..5277507 (-) 723 WP_304351790.1 YggS family pyridoxal phosphate-dependent enzyme -
  U0024_RS24105 (U0024_24110) pilT 5277545..5278588 (+) 1044 WP_302088810.1 type IV pilus twitching motility protein PilT Machinery gene
  U0024_RS24110 (U0024_24115) pilU 5278712..5279848 (+) 1137 WP_302088809.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U0024_RS24115 (U0024_24120) - 5280008..5280919 (+) 912 WP_304351791.1 NAD(P)-dependent oxidoreductase -
  U0024_RS24120 (U0024_24125) - 5280981..5281634 (-) 654 WP_302088807.1 BON domain-containing protein -
  U0024_RS24125 (U0024_24130) - 5281631..5282239 (-) 609 WP_043375490.1 SIS domain-containing protein -
  U0024_RS24130 (U0024_24135) - 5282329..5282730 (-) 402 WP_302088806.1 YraN family protein -
  U0024_RS24135 (U0024_24140) rsmI 5282730..5283653 (+) 924 WP_304351792.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41990.48 Da        Isoelectric Point: 6.7686

>NTDB_id=912308 U0024_RS24110 WP_302088809.1 5278712..5279848(+) (pilU) [Comamonas testosteroni strain HAMBI_0403]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGKVTKVSPQPLSPLHTLALARSIMNDRQVADFEHTKECNFAI
SPAGVGRFRVNAFMQQGKVGMVLRTIPTTLPTIDGLGVPQVLKEVTMTKRGLCVLVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDVSLNLRAIISQRLIPKQDSKGRVAAIEVMLNSPLIADLIFKGEVVEIKEIMKKSRNIG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLNSQRARTLDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=912308 U0024_RS24110 WP_302088809.1 5278712..5279848(+) (pilU) [Comamonas testosteroni strain HAMBI_0403]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCGGAGTTTCCGCCCGCCATCAAGATTGACGGCAAGGTCACCAAGGTCTCGCCCCAGCCGCTGAGCCCGCTGCATA
CGCTGGCCCTGGCGCGCTCCATCATGAACGACAGGCAGGTGGCGGACTTCGAGCACACCAAGGAGTGCAACTTCGCCATC
TCGCCGGCCGGCGTGGGTCGCTTCCGCGTCAATGCCTTCATGCAGCAGGGCAAGGTCGGCATGGTGCTGCGTACCATTCC
CACCACCCTGCCCACGATTGACGGCCTGGGCGTGCCGCAGGTGCTCAAGGAAGTGACCATGACCAAGCGCGGCCTGTGCG
TCCTGGTCGGCGCGACGGGCTCGGGCAAGTCCACCACGCTGGCTGCCATGGTGGACTGGCGCAATGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCCGTGGAATTCGTGCATCCCCACAAGAACTGCGTAGTCACGCAACGCGAGGTGGGGCT
GGATACGGACAGCTGGGAAGCGGCACTCAAGAACACGCTGCGCCAGGCTCCCGATGTGATTTTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCGATTGCGTTCTCTGAAACCGGTCACCTGTGCCTGGCCACACTGCACGCCAACAGTGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAAGAGCGTCGCCCGCAACTGCTGATGGACGTTTCGCTGAATCT
GCGGGCCATCATTTCCCAGCGCCTGATCCCCAAGCAGGACAGCAAGGGCCGTGTAGCGGCCATCGAGGTGATGCTCAACT
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGAGGTCGTCGAGATCAAGGAGATCATGAAGAAAAGCCGCAATATCGGC
ATGCAGACCTTTGACCAGGCGCTGTTCGACCTGTTCGAATCCAATCTCATCACCTACGAAGATGCGCTGCGCAATGCAGA
CTCGGTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCCAGCGCGCCCGGACTCTGGACCTGGCAGCCGGCACAGAGC
ATCTGACTATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.749

96.825

0.598
  pilU Acinetobacter baylyi ADP1

55.989

94.974

0.532
  pilU Vibrio cholerae strain A1552

54.023

92.063

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362
  pilT Acinetobacter baylyi ADP1

41.768

86.772

0.362