Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U0004_RS05240 Genome accession   NZ_CP139976
Coordinates   1166044..1167087 (-) Length   347 a.a.
NCBI ID   WP_070258779.1    Uniprot ID   A0A1G8GTH2
Organism   Janthinobacterium lividum strain HAMBI_1919     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1161044..1172087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0004_RS05225 (U0004_05225) - 1162116..1164227 (-) 2112 WP_070258775.1 putative bifunctional diguanylate cyclase/phosphodiesterase -
  U0004_RS05230 (U0004_05230) - 1164414..1164899 (-) 486 WP_070258777.1 glutathione peroxidase -
  U0004_RS05235 (U0004_05235) pilU 1164901..1166040 (-) 1140 WP_174718092.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U0004_RS05240 (U0004_05240) pilT 1166044..1167087 (-) 1044 WP_070258779.1 type IV pilus twitching motility protein PilT Machinery gene
  U0004_RS05245 (U0004_05245) - 1167158..1167859 (+) 702 WP_070258914.1 YggS family pyridoxal phosphate-dependent enzyme -
  U0004_RS05250 (U0004_05250) proC 1167882..1168712 (+) 831 WP_034784843.1 pyrroline-5-carboxylate reductase -
  U0004_RS05255 (U0004_05255) - 1168713..1169429 (-) 717 WP_115057411.1 anti-sigma factor domain-containing protein -
  U0004_RS05260 (U0004_05260) - 1169426..1169983 (-) 558 WP_070258918.1 sigma-70 family RNA polymerase sigma factor -
  U0004_RS05265 (U0004_05265) - 1170076..1170633 (-) 558 WP_034784851.1 DUF3455 domain-containing protein -
  U0004_RS05270 (U0004_05270) - 1170723..1171073 (-) 351 WP_070258781.1 hypothetical protein -
  U0004_RS05275 (U0004_05275) - 1171070..1171456 (-) 387 WP_034784855.1 phage holin family protein -
  U0004_RS05280 (U0004_05280) - 1171524..1171880 (-) 357 WP_034784857.1 DUF883 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38059.57 Da        Isoelectric Point: 6.6380

>NTDB_id=912186 U0004_RS05240 WP_070258779.1 1166044..1167087(-) (pilT) [Janthinobacterium lividum strain HAMBI_1919]
MDISELLAFSVSNKASDLHLSSGLPPMIRVNGDVRRLNVAPLEHKEVHSMIYDIMNDSQRKAYEEALECDFSFEIPGLAR
FRVNAYNQERGASAVLRTIPSKVLTLEELNAPRIFGELAMRPRGLVLVTGPTGSGKSTTLAAMVNHVNERLNHHILTIED
PIEFVHEPKKCLINQREVGSHTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKSIDRI
IDVFPAEEKEMVRAMLSESLQAVISQNLLKTKDGAGRVAAHEIMLATPAVRNLIREAKVAQMYSAIQTGSNVGMQTLDQC
LTDLVRRGTISAETARSAAKAPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=912186 U0004_RS05240 WP_070258779.1 1166044..1167087(-) (pilT) [Janthinobacterium lividum strain HAMBI_1919]
ATGGACATCTCCGAACTACTCGCTTTCTCCGTCAGCAACAAGGCTTCCGACTTGCACCTGTCTTCCGGCCTGCCGCCGAT
GATACGGGTCAACGGCGACGTGCGCCGCCTGAACGTGGCGCCGCTCGAGCACAAGGAAGTGCACAGCATGATCTACGACA
TCATGAACGACAGCCAGCGCAAGGCGTATGAAGAAGCGCTGGAATGCGATTTTTCGTTCGAGATTCCCGGCCTGGCCCGC
TTCCGCGTCAACGCCTACAACCAGGAACGCGGTGCCTCGGCCGTGCTGCGCACGATTCCGTCGAAGGTGCTGACCCTGGA
AGAACTCAACGCCCCGCGCATCTTCGGCGAGCTGGCCATGCGCCCGCGGGGACTCGTGCTGGTGACGGGCCCGACGGGCT
CCGGCAAGTCGACCACCCTGGCGGCCATGGTGAACCACGTCAACGAGCGCCTGAACCACCATATATTGACCATCGAAGAC
CCGATCGAATTCGTGCATGAACCGAAGAAATGCCTGATCAACCAGCGCGAAGTGGGCTCGCACACGCATTCGTTCAGCAA
TGCGCTGCGCTCGGCCCTGCGCGAAGACCCCGACGTGATTTTGGTGGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAGACGGGCCACCTCGTGTTCGGCACCCTGCATACCTCGTCGGCGGCGAAATCGATCGACCGCATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCCTTGCAAGCCGTCATTTCGCAGAA
CTTGCTGAAAACCAAGGATGGCGCGGGCCGCGTGGCCGCGCATGAAATCATGCTGGCCACGCCGGCCGTGCGCAACCTGA
TCCGCGAAGCAAAAGTGGCGCAAATGTATTCGGCCATCCAGACGGGCAGCAACGTGGGCATGCAGACCCTGGACCAGTGC
CTCACCGACCTGGTGCGGCGCGGCACGATCTCGGCGGAAACAGCCCGCTCGGCCGCCAAGGCGCCTGAAAACTTCCCCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G8GTH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

71.681

97.695

0.7
  pilT Legionella pneumophila strain ERS1305867

71.681

97.695

0.7
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.118

0.683
  pilT Vibrio cholerae strain A1552

70.326

97.118

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.424

0.522
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

40.58

99.424

0.403