Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U0036_RS14790 Genome accession   NZ_CP139967
Coordinates   3175402..3176445 (-) Length   347 a.a.
NCBI ID   WP_063240245.1    Uniprot ID   A0A4R5JAE5
Organism   Cupriavidus oxalaticus strain HAMBI_2164     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3170402..3181445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0036_RS14760 (U0036_14765) - 3171312..3171674 (+) 363 WP_063240239.1 CidA/LrgA family protein -
  U0036_RS14765 (U0036_14770) - 3171671..3172396 (+) 726 WP_063240240.1 LrgB family protein -
  U0036_RS14770 (U0036_14775) - 3172493..3172864 (+) 372 WP_198065752.1 VOC family protein -
  U0036_RS14775 (U0036_14780) - 3172911..3173600 (+) 690 WP_063240242.1 helix-turn-helix transcriptional regulator -
  U0036_RS14780 (U0036_14785) - 3173623..3174117 (-) 495 WP_063240243.1 glutathione peroxidase -
  U0036_RS14785 (U0036_14790) pilU 3174193..3175332 (-) 1140 WP_063240244.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U0036_RS14790 (U0036_14795) pilT 3175402..3176445 (-) 1044 WP_063240245.1 type IV pilus twitching motility protein PilT Machinery gene
  U0036_RS14795 (U0036_14800) - 3176511..3177200 (+) 690 WP_063240246.1 YggS family pyridoxal phosphate-dependent enzyme -
  U0036_RS14800 (U0036_14805) proC 3177241..3178074 (+) 834 WP_063240258.1 pyrroline-5-carboxylate reductase -
  U0036_RS14805 (U0036_14810) ubiA 3179055..3179912 (-) 858 WP_063242106.1 4-hydroxybenzoate octaprenyltransferase -
  U0036_RS14810 (U0036_14815) - 3180054..3180539 (-) 486 WP_063242105.1 Dps family protein -
  U0036_RS14815 (U0036_14820) - 3180633..3180827 (-) 195 WP_063242104.1 hypothetical protein -
  U0036_RS30475 - 3180874..3181065 (-) 192 WP_084254817.1 catalase-related domain-containing protein -
  U0036_RS14820 (U0036_14825) - 3180992..3181165 (-) 174 Protein_2915 catalase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38405.20 Da        Isoelectric Point: 6.9772

>NTDB_id=912070 U0036_RS14790 WP_063240245.1 3175402..3176445(-) (pilT) [Cupriavidus oxalaticus strain HAMBI_2164]
MDIAQLLAFAVKNKASDLHLSSDMPPMVRIHGDMRRINVAAMAHKDVHAMVYDIMSDTQRKLYEERLEIDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVED
PIEFVHNSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSAISYNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=912070 U0036_RS14790 WP_063240245.1 3175402..3176445(-) (pilT) [Cupriavidus oxalaticus strain HAMBI_2164]
ATGGACATCGCGCAGCTATTGGCTTTCGCCGTCAAGAACAAGGCGTCCGATCTTCATCTGTCATCGGACATGCCGCCGAT
GGTCCGGATCCACGGCGACATGCGCCGGATCAATGTCGCGGCGATGGCGCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGCTCTACGAAGAACGGCTGGAAATCGACTTCTCGTTCGAGATCGCCGGCCTGTCGCGC
TTCCGGGTCAATGCCTACAACACGCAGCGCGGCGCCGCCGCGGTGTTCCGTACCATTCCCTCGAAGGTCCTGACGCTGGA
AGAGCTGCGCGCGCCGGCGGTGTTCGCCGACCTGTGCATGAAGCCGCGCGGGCTGGTGCTGGTGACGGGGCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTCGACCATCGCAATGAAAACGACATGGGTCACATCCTGACGGTGGAAGAC
CCGATCGAATTCGTCCACAACTCCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGGCCGCACACGCATTCGTTCGCCAA
CGCGCTGCGCTCGGCGCTGCGTGAAGATCCGGACGTGATCCTCGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAAACCGGCCACCTGGTCTTCGGCACGCTGCACACCAGTTCCGCGGCCAAGACCATCGACCGTGTG
GTCGACGTGTTCCCGCCGGAAGAAAAGGACATGGTGCGCACCATGCTGTCCGAATCGCTGGAAGCGGTGATCTCGCAGAC
GCTCTTGAAGACCCGCGACGGCAACGGCCGTACCGCCGCGCACGAGATCATGATCGCCACCCCGGCGATCCGGCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCAGCGCCATCAGCTACAACGATGCGCGCGCCATCGCCAAGAACCCCGACGCGTTCATGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R5JAE5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.676

99.712

0.715
  pilT Acinetobacter baumannii D1279779

71.676

99.712

0.715
  pilT Acinetobacter baumannii strain A118

71.676

99.712

0.715
  pilT Pseudomonas stutzeri DSM 10701

70.435

99.424

0.7
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Pseudomonas aeruginosa PAK

69.565

99.424

0.692
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Legionella pneumophila strain Lp02

66.667

99.424

0.663
  pilT Legionella pneumophila strain ERS1305867

66.667

99.424

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.282

97.118

0.634
  pilT Vibrio cholerae strain A1552

65.282

97.118

0.634
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

99.424

0.49
  pilU Acinetobacter baylyi ADP1

39.826

99.135

0.395
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389