Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U0036_RS14785 Genome accession   NZ_CP139967
Coordinates   3174193..3175332 (-) Length   379 a.a.
NCBI ID   WP_063240244.1    Uniprot ID   -
Organism   Cupriavidus oxalaticus strain HAMBI_2164     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3169193..3180332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0036_RS14755 (U0036_14760) glcF 3169869..3171122 (+) 1254 WP_063240238.1 glycolate oxidase subunit GlcF -
  U0036_RS14760 (U0036_14765) - 3171312..3171674 (+) 363 WP_063240239.1 CidA/LrgA family protein -
  U0036_RS14765 (U0036_14770) - 3171671..3172396 (+) 726 WP_063240240.1 LrgB family protein -
  U0036_RS14770 (U0036_14775) - 3172493..3172864 (+) 372 WP_198065752.1 VOC family protein -
  U0036_RS14775 (U0036_14780) - 3172911..3173600 (+) 690 WP_063240242.1 helix-turn-helix transcriptional regulator -
  U0036_RS14780 (U0036_14785) - 3173623..3174117 (-) 495 WP_063240243.1 glutathione peroxidase -
  U0036_RS14785 (U0036_14790) pilU 3174193..3175332 (-) 1140 WP_063240244.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U0036_RS14790 (U0036_14795) pilT 3175402..3176445 (-) 1044 WP_063240245.1 type IV pilus twitching motility protein PilT Machinery gene
  U0036_RS14795 (U0036_14800) - 3176511..3177200 (+) 690 WP_063240246.1 YggS family pyridoxal phosphate-dependent enzyme -
  U0036_RS14800 (U0036_14805) proC 3177241..3178074 (+) 834 WP_063240258.1 pyrroline-5-carboxylate reductase -
  U0036_RS14805 (U0036_14810) ubiA 3179055..3179912 (-) 858 WP_063242106.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42149.38 Da        Isoelectric Point: 6.6383

>NTDB_id=912069 U0036_RS14785 WP_063240244.1 3174193..3175332(-) (pilU) [Cupriavidus oxalaticus strain HAMBI_2164]
MLDRESAAKYINDLLELMVSNRGSDLFITADFPPAIKVDGKITPVSQQPLNPTQALGLVRSVMNERQVQDFDTSRECNFA
ITAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVADLDLPPTLHDIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCVVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLYEEGKITYEDALRNADSLNDLRLMIKLHSARARDADLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=912069 U0036_RS14785 WP_063240244.1 3174193..3175332(-) (pilU) [Cupriavidus oxalaticus strain HAMBI_2164]
ATGCTCGATCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTGGAGCTGATGGTCAGCAACCGCGGCTCGGACCTGTT
CATCACCGCCGACTTCCCGCCCGCGATCAAGGTGGACGGCAAGATCACGCCGGTGTCGCAGCAGCCGCTGAACCCGACCC
AGGCGCTGGGCCTGGTGCGCTCGGTAATGAACGAGCGCCAGGTGCAGGACTTCGACACCAGCCGCGAATGCAACTTCGCC
ATCACCGCGCCCAAGGCCGGGCGCTTCCGCGTGTCGGCGTTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGGACCAT
CAATACGCGGATTCCGTCGGTGGCGGACCTGGACCTGCCTCCGACGCTGCACGACATCGTGATGTCCAAGCGCGGGCTGG
TGATCGTGACCGGCGCGACCGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGCTCGACCACCGCAACGCGCATTCCTAC
GGCCACATCATCACCATCGAGGATCCGATCGAATACGTGCATGCGCACCAGAACTGCGTCGTCACGCAGCGCGAGGTCGG
CATCGATACGGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGACCGCGAGACCATGGAATACGCGATGCAGTACGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCGATCGACCGCGTGGTCAACTTCTTCCCCGAAGAGAAGCGCCAGCAACTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGGTGTCGCAGCGCCTGCTGCCGCGCGCCGGGCGCAAGGGCCGCGTGCCGGCCGTGGAAATCATGATCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAACTGAAGGAAGTCATCAAGAAATCACGCGAGCAG
GGCATGATCTCGTTCGACCAGGCGCTGTTCGACCTGTACGAGGAAGGCAAGATCACCTACGAGGACGCGCTGCGCAATGC
CGACTCGCTCAATGACCTGCGCCTGATGATCAAGCTGCACAGCGCGCGCGCCAGGGACGCCGACCTGGGCGCCGGCACCG
AGCACCTGAACGTGATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(53-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.218

91.821

0.58
  pilU Acinetobacter baylyi ADP1

58

92.348

0.536
  pilU Vibrio cholerae strain A1552

52.893

95.778

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

88.918

0.401
  pilT Acinetobacter baumannii D1279779

43.544

87.863

0.383
  pilT Acinetobacter nosocomialis M2

43.544

87.863

0.383
  pilT Acinetobacter baumannii strain A118

43.544

87.863

0.383
  pilT Pseudomonas aeruginosa PAK

43.027

88.918

0.383
  pilT Pseudomonas stutzeri DSM 10701

43.027

88.918

0.383
  pilT Acinetobacter baylyi ADP1

43.243

87.863

0.38
  pilT Legionella pneumophila strain Lp02

42.943

87.863

0.377
  pilT Legionella pneumophila strain ERS1305867

42.943

87.863

0.377
  pilT Neisseria meningitidis 8013

40.294

89.71

0.361