Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PCI15_RS02020 Genome accession   NZ_AP025281
Coordinates   457572..458606 (+) Length   344 a.a.
NCBI ID   WP_271272706.1    Uniprot ID   -
Organism   Aliamphritea hakodatensis strain PT3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452572..463606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCI15_RS01990 hemW 452780..453940 (-) 1161 WP_271272700.1 radical SAM family heme chaperone HemW -
  PCI15_RS01995 rdgB 453986..454582 (-) 597 WP_271272701.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  PCI15_RS02000 - 454605..455066 (-) 462 WP_271272702.1 DUF4426 domain-containing protein -
  PCI15_RS02005 - 455232..455789 (-) 558 WP_271272703.1 YggT family protein -
  PCI15_RS02010 proC 455872..456696 (-) 825 WP_271272704.1 pyrroline-5-carboxylate reductase -
  PCI15_RS02015 - 456751..457455 (-) 705 WP_271272705.1 YggS family pyridoxal phosphate-dependent enzyme -
  PCI15_RS02020 pilT 457572..458606 (+) 1035 WP_271272706.1 type IV pilus twitching motility protein PilT Machinery gene
  PCI15_RS02025 pilU 458734..459897 (+) 1164 WP_271272707.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PCI15_RS02030 - 459963..460577 (-) 615 WP_271272708.1 TetR/AcrR family transcriptional regulator -
  PCI15_RS02035 - 460748..461728 (+) 981 WP_271272709.1 fatty acid desaturase -
  PCI15_RS02040 - 461750..462061 (+) 312 WP_271272710.1 MocE family 2Fe-2S type ferredoxin -
  PCI15_RS02045 - 462157..462714 (+) 558 WP_271272711.1 dienelactone hydrolase family protein -
  PCI15_RS02050 - 462780..463028 (-) 249 WP_271272712.1 YfhL family 4Fe-4S dicluster ferredoxin -
  PCI15_RS02055 coaD 463055..463534 (-) 480 WP_271272713.1 pantetheine-phosphate adenylyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38153.94 Da        Isoelectric Point: 6.2462

>NTDB_id=90986 PCI15_RS02020 WP_271272706.1 457572..458606(+) (pilT) [Aliamphritea hakodatensis strain PT3]
MDITELLSFTVQQGASDLHISAGMPPVIRVDGEVRRIKLPSLDHKQVHTLIYDIMNDKQRKDYEEILETDFSFEVPKLAR
FRVNAFNQNRGAAAVFRTIPSKVLTMEDLGMGKVFQDLAEKPRGLVLVTGPTGSGKSTTLAAMVDYINETRQDHILTIED
PIEFVHESKKSLINQREVHRDTLGFDEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAAEKAMVRSMLSESLQGVISQTLLKKPMGGRVAAHEIMVGTPAIRNLIREDKIAQMYSAIQTGAAFGMVTLDQSL
TDLLNKGLISREAAREKAMNPMNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=90986 PCI15_RS02020 WP_271272706.1 457572..458606(+) (pilT) [Aliamphritea hakodatensis strain PT3]
ATGGATATTACCGAGTTACTTTCATTCACGGTTCAGCAAGGCGCGTCTGACTTACACATTTCTGCCGGTATGCCTCCGGT
GATCCGGGTCGACGGTGAAGTGCGGCGGATTAAGCTGCCTTCTCTGGATCATAAACAGGTTCACACGCTGATATATGACA
TTATGAATGATAAGCAGCGTAAGGATTATGAAGAGATCTTGGAAACGGATTTCTCTTTTGAAGTACCTAAGCTGGCACGT
TTCCGTGTTAACGCTTTTAATCAGAACCGCGGTGCAGCGGCGGTATTCCGGACCATCCCGAGTAAAGTGCTGACGATGGA
AGATCTGGGGATGGGGAAGGTCTTTCAGGATTTGGCAGAAAAACCCCGCGGACTGGTGCTGGTGACCGGGCCGACCGGTT
CCGGTAAAAGTACCACCCTGGCGGCTATGGTCGATTACATCAATGAAACCCGTCAGGATCATATTCTGACCATTGAAGAC
CCGATCGAATTTGTTCACGAGAGTAAAAAGAGCCTGATTAACCAGCGGGAAGTACACCGCGACACTCTGGGCTTTGATGA
AGCGCTGCGCTCTGCTTTACGTGAAGACCCTGATGTTATCCTCGTCGGTGAGATGCGGGACCTTGAAACCATTCGCCTGG
CACTGACCGCGGCGGAAACCGGTCATCTGGTGTTCGGCACCCTGCACACCACCTCAGCCGCTAAAACCATTGACCGTGTT
GTGGATGTATTCCCGGCAGCTGAGAAAGCCATGGTACGTTCGATGCTGTCAGAGTCGCTGCAGGGGGTTATTTCCCAGAC
GCTGCTGAAAAAGCCGATGGGTGGCCGGGTCGCTGCCCATGAAATTATGGTGGGCACGCCGGCGATCCGTAACCTGATCC
GGGAAGACAAAATCGCACAAATGTATTCTGCAATACAAACCGGTGCTGCATTTGGTATGGTTACCCTGGATCAGTCGCTG
ACAGATCTGCTTAATAAAGGCCTGATCAGCCGCGAAGCCGCGCGCGAAAAGGCTATGAATCCAATGAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

83.721

100

0.837
  pilT Pseudomonas stutzeri DSM 10701

83.14

100

0.831
  pilT Acinetobacter nosocomialis M2

81.395

100

0.814
  pilT Acinetobacter baumannii D1279779

81.395

100

0.814
  pilT Acinetobacter baumannii strain A118

81.395

100

0.814
  pilT Acinetobacter baylyi ADP1

80.523

100

0.805
  pilT Legionella pneumophila strain Lp02

73.547

100

0.735
  pilT Legionella pneumophila strain ERS1305867

73.547

100

0.735
  pilT Neisseria gonorrhoeae MS11

68.328

99.128

0.677
  pilT Neisseria meningitidis 8013

68.328

99.128

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.965

0.657
  pilT Vibrio cholerae strain A1552

67.062

97.965

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387

Multiple sequence alignment