Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PCI15_RS02025 Genome accession   NZ_AP025281
Coordinates   458734..459897 (+) Length   387 a.a.
NCBI ID   WP_271272707.1    Uniprot ID   -
Organism   Aliamphritea hakodatensis strain PT3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453734..464897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCI15_RS01995 rdgB 453986..454582 (-) 597 WP_271272701.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  PCI15_RS02000 - 454605..455066 (-) 462 WP_271272702.1 DUF4426 domain-containing protein -
  PCI15_RS02005 - 455232..455789 (-) 558 WP_271272703.1 YggT family protein -
  PCI15_RS02010 proC 455872..456696 (-) 825 WP_271272704.1 pyrroline-5-carboxylate reductase -
  PCI15_RS02015 - 456751..457455 (-) 705 WP_271272705.1 YggS family pyridoxal phosphate-dependent enzyme -
  PCI15_RS02020 pilT 457572..458606 (+) 1035 WP_271272706.1 type IV pilus twitching motility protein PilT Machinery gene
  PCI15_RS02025 pilU 458734..459897 (+) 1164 WP_271272707.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PCI15_RS02030 - 459963..460577 (-) 615 WP_271272708.1 TetR/AcrR family transcriptional regulator -
  PCI15_RS02035 - 460748..461728 (+) 981 WP_271272709.1 fatty acid desaturase -
  PCI15_RS02040 - 461750..462061 (+) 312 WP_271272710.1 MocE family 2Fe-2S type ferredoxin -
  PCI15_RS02045 - 462157..462714 (+) 558 WP_271272711.1 dienelactone hydrolase family protein -
  PCI15_RS02050 - 462780..463028 (-) 249 WP_271272712.1 YfhL family 4Fe-4S dicluster ferredoxin -
  PCI15_RS02055 coaD 463055..463534 (-) 480 WP_271272713.1 pantetheine-phosphate adenylyltransferase -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43058.14 Da        Isoelectric Point: 5.1664

>NTDB_id=90987 PCI15_RS02025 WP_271272707.1 458734..459897(+) (pilU) [Aliamphritea hakodatensis strain PT3]
MDITQLLKIMNDRGASDLFVTAGARPSIKVDGTLKPLTKEALKPDQARKLTCSTMNDQQLVEFESTRECNFAISAQGLGR
YRVSAFYQRNSPGMVLRRINTRIPTMEELNLPAVLKDLAMAKRGLILFVGGTSTGKSTSLASMIDYRNSNSGGHIITIED
PIEYIHDHKQSLITQREVGLDTDSFEVALKNTLRQAPDVILMGEIRSSDTMGYGLSFAETGHLCMATMHANNANQALDRI
ISFFPPESHAQVWMDLSLNLKAIVAQQLLPTRDGKGRRAVIEVMINTPLIQDLIRKGEVHELKEVIKKSSNLGMQTFDQA
LYDTYKEGIINYDVALAHADSANDLRLMIKLNATESEVVPEDDTMNFSLQDEDDYIANEGSIEINKM

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=90987 PCI15_RS02025 WP_271272707.1 458734..459897(+) (pilU) [Aliamphritea hakodatensis strain PT3]
GTGGATATTACTCAGCTCTTAAAGATTATGAATGATCGCGGTGCCTCAGACCTGTTTGTGACCGCAGGTGCCAGGCCGAG
TATCAAGGTCGATGGTACGCTGAAGCCGTTAACCAAGGAAGCGTTAAAGCCTGATCAGGCCCGTAAACTGACCTGCAGTA
CGATGAATGACCAGCAACTGGTTGAGTTTGAAAGTACCCGCGAGTGTAACTTTGCGATCAGCGCTCAGGGATTAGGCCGT
TACCGGGTGAGTGCGTTTTATCAGCGTAACTCACCGGGGATGGTGTTACGGCGTATCAATACCCGCATACCGACCATGGA
AGAGCTGAACCTGCCGGCTGTGCTTAAAGATCTGGCGATGGCCAAGCGGGGGCTTATTCTGTTTGTGGGTGGCACCAGTA
CCGGTAAATCCACCTCGCTGGCGTCGATGATTGATTACCGTAACAGCAACAGCGGCGGCCATATCATCACCATTGAAGAC
CCGATTGAATATATCCATGATCACAAACAAAGCCTGATTACACAGCGTGAGGTTGGGCTGGATACTGACTCTTTTGAGGT
CGCACTGAAGAATACCCTGCGCCAGGCACCGGATGTGATCCTGATGGGGGAGATCCGCAGCTCCGATACTATGGGGTACG
GTCTGTCGTTCGCTGAAACCGGCCACCTGTGTATGGCAACCATGCACGCTAACAATGCTAACCAGGCGCTGGACCGGATT
ATCAGTTTCTTCCCGCCGGAAAGCCATGCGCAGGTCTGGATGGATCTGTCCCTGAACCTTAAGGCGATTGTTGCCCAGCA
GCTGCTGCCAACCCGTGACGGTAAAGGTCGCCGGGCGGTGATTGAAGTGATGATCAATACGCCGCTGATTCAGGATTTGA
TCCGTAAGGGCGAAGTGCATGAGCTCAAAGAGGTTATCAAGAAGTCGTCTAATCTGGGCATGCAGACCTTTGATCAGGCA
CTGTACGACACCTACAAGGAAGGCATTATCAATTATGATGTGGCACTGGCCCATGCGGACTCAGCCAACGACCTGCGCCT
GATGATCAAGCTGAATGCCACTGAGTCTGAAGTAGTGCCTGAAGATGATACCATGAACTTTTCGCTGCAGGATGAAGATG
ATTACATCGCCAATGAAGGCAGCATTGAAATCAATAAAATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.555

91.214

0.625
  pilU Acinetobacter baylyi ADP1

60.623

91.214

0.553
  pilU Vibrio cholerae strain A1552

57.714

90.439

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

87.597

0.385
  pilT Acinetobacter baylyi ADP1

41.935

88.114

0.37
  pilT Pseudomonas aeruginosa PAK

42.09

86.563

0.364
  pilT Legionella pneumophila strain ERS1305867

41.791

86.563

0.362
  pilT Pseudomonas stutzeri DSM 10701

41.791

86.563

0.362
  pilT Acinetobacter nosocomialis M2

41.791

86.563

0.362
  pilT Acinetobacter baumannii D1279779

41.791

86.563

0.362
  pilT Acinetobacter baumannii strain A118

41.791

86.563

0.362
  pilT Legionella pneumophila strain Lp02

41.791

86.563

0.362

Multiple sequence alignment