Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SM130_RS01350 Genome accession   NZ_CP139348
Coordinates   308007..309041 (+) Length   344 a.a.
NCBI ID   WP_102824048.1    Uniprot ID   A0A2N8S3S6
Organism   Stutzerimonas stutzeri strain KC     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 303007..314041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM130_RS01330 (SM130_01330) - 303580..305547 (-) 1968 WP_102824043.1 dynamin-like GTPase family protein -
  SM130_RS01335 (SM130_01335) - 305816..306406 (-) 591 WP_102824045.1 YggT family protein -
  SM130_RS01340 (SM130_01340) proC 306416..307240 (-) 825 WP_102824046.1 pyrroline-5-carboxylate reductase -
  SM130_RS01345 (SM130_01345) - 307251..307940 (-) 690 WP_102824047.1 YggS family pyridoxal phosphate-dependent enzyme -
  SM130_RS01350 (SM130_01350) pilT 308007..309041 (+) 1035 WP_102824048.1 type IV pilus twitching motility protein PilT Machinery gene
  SM130_RS01355 (SM130_01355) pilU 309101..310246 (+) 1146 WP_102824049.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SM130_RS01360 (SM130_01360) - 310243..311079 (-) 837 WP_102824050.1 TIGR03915 family putative DNA repair protein -
  SM130_RS01365 (SM130_01365) - 311079..312290 (-) 1212 WP_102824051.1 putative DNA modification/repair radical SAM protein -
  SM130_RS01370 (SM130_01370) - 312424..312819 (+) 396 WP_102824052.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37932.65 Da        Isoelectric Point: 6.8360

>NTDB_id=909019 SM130_RS01350 WP_102824048.1 308007..309041(+) (pilT) [Stutzerimonas stutzeri strain KC]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDSCL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=909019 SM130_RS01350 WP_102824048.1 308007..309041(+) (pilT) [Stutzerimonas stutzeri strain KC]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCGGATTTGCACCTTTCCTCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGAATCAACCTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAGTTCCTGGAAACTGACTTCTCCTTCGAAGTGCCGGGCGTCGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGTGCTGGCGCCGTATTCCGGACCATTCCTTCCAAGGTTCTGACCATGGA
AGACCTGGGGATGGGCGAGGTGTTTCGCAAGATCACCGACGTACCTCGGGGCTTGGTACTGGTTACCGGGCCGACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGATTACCTGAACAACACCAAGTACCACCACATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAAGTGCACCGCGATACGTTGGGCTTCTCCGA
AGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATCCTGGTGGGTGAAATGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCTGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAAGAGAAGTCCATGGTTCGCTCGATGCTTTCCGAGTCGCTACAGGCGGTAGTGTCCCAGAC
GCTGCTGAAGAAGGTAGGCGGCGGCCGTGTGGCGGCGCACGAGATCATGATCGGTACTCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTTGCGCAGATGTATTCCTCGATTCAGACCGGTGGCTCGCTGGGCATGCAGACGCTCGACTCGTGTCTC
AAGGGGCTGCTCGCCAAAGGGCTGATCTCGCGAGACAGCGCCAAGGAAAAGGCCAAGCAGCCAGAGAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8S3S6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.709

100

0.997
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39