Detailed information NTG0909020

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SM130_RS01355
• Genome accession: NZ_CP139348
• Coordinates: 309101..310246 (+)
• Length: 381 a.a.
• NCBI ID: WP_102824049.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain KC
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 304101..315246

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SM130_RS01335 (SM130_01335)	-	305816..306406 (-)	591	WP_102824045.1	YggT family protein	-
SM130_RS01340 (SM130_01340)	proC	306416..307240 (-)	825	WP_102824046.1	pyrroline-5-carboxylate reductase	-
SM130_RS01345 (SM130_01345)	-	307251..307940 (-)	690	WP_102824047.1	YggS family pyridoxal phosphate-dependent enzyme	-
SM130_RS01350 (SM130_01350)	pilT	308007..309041 (+)	1035	WP_102824048.1	type IV pilus twitching motility protein PilT	Machinery gene
SM130_RS01355 (SM130_01355)	pilU	309101..310246 (+)	1146	WP_102824049.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SM130_RS01360 (SM130_01360)	-	310243..311079 (-)	837	WP_102824050.1	TIGR03915 family putative DNA repair protein	-
SM130_RS01365 (SM130_01365)	-	311079..312290 (-)	1212	WP_102824051.1	putative DNA modification/repair radical SAM protein	-
SM130_RS01370 (SM130_01370)	-	312424..312819 (+)	396	WP_102824052.1	TM2 domain-containing protein	-
SM130_RS01375 (SM130_01375)	-	312896..314170 (-)	1275	WP_102824053.1	dihydroorotase	-
SM130_RS01380 (SM130_01380)	-	314170..315186 (-)	1017	WP_102824054.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42444.70 Da
• Isoelectric Point: 6.7256

>NTDB_id=909020 SM130_RS01355 WP_102824049.1 309101..310246(+) (pilU) [Stutzerimonas stutzeri strain KC]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIDELKLPDILKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRSFR

Nucleotide

• Length: 1146 bp

>NTDB_id=909020 SM130_RS01355 WP_102824049.1 309101..310246(+) (pilU) [Stutzerimonas stutzeri strain KC]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGATCATGCCAGTGAGCAAGACCGCCATGTCACCGGAAATGACCCGGGAAACCGTGCATGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAAAACCACGAGTGCAACTTCGCTATCAGCGCCCGCGGTATTGGCCGC
TTTCGCGTCAGCGCTTTCTACCAGCGCAACCTCGCCGGGATGGTGTTGCGTCGCATCGAGACCAACATTCCCACGATCGA
CGAGCTCAAACTGCCCGACATCCTCAAGAAGCTGTCGATGACCAAGCGCGGCCTGGTGCTGTTCGTTGGCGCCACGGGCA
CCGGCAAGTCGACCTCGTTGGCGTCGATGATCGGCTACCGCAACAAGAATTCCAGCGGGCACATCATTTCCATCGAGGAC
CCGATCGAGTTCATCCACCAGCACCAGAGCTGCATCGTCACCCAGCGTGAGGTGGGGATCGACACCAGCTCGTTCGAGGT
GGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAAATTCGTACCCGTGAAACCATGGACTACG
CCGTGGCGTTCGCCGAAACCGGGCACTTGTGCCTGGCGACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCCGACCGTCACAACCAGGTGTGGATGGACCTTTCGCTCAACCTCAAGGCGATCGTCGCTCAGCA
GCTGGTGCCGACACCGGATGGCAAGGGGCGCCGCGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCAGCGGACCTGA
TCCGCAAAGGCGAGGTTCATGAGCTCAAGTCGCTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGATCAGGCG
CTGTACAACCTCTACGTGCAGGGCGAGATCACCTACGAAGATGCGTTGCTGCACGCCGACTCAGCCAACGACCTGCGCCT
GCTGATCAAGCTGGGTTCGGAAACCGATGGCGAGCATCTGACTAGTGTGTCGCAAGGCCTGAGCCTTGAAGTCAGTGACG
AAGACCCGGGGCGCAGTTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.226	100	0.942
pilU	Acinetobacter baylyi ADP1	66.667	93.701	0.625
pilU	Vibrio cholerae strain A1552	59.143	91.864	0.543
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388