Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SJN48_RS15440 Genome accession   NZ_CP139212
Coordinates   3259123..3260160 (+) Length   345 a.a.
NCBI ID   WP_002121749.1    Uniprot ID   A0A009I411
Organism   Acinetobacter pittii strain WWTP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3254123..3265160
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SJN48_RS15430 (SJN48_15480) sbcC 3254507..3258103 (+) 3597 WP_130121012.1 AAA family ATPase -
  SJN48_RS15435 (SJN48_15485) yggS 3258304..3258996 (-) 693 WP_130121013.1 YggS family pyridoxal phosphate-dependent enzyme -
  SJN48_RS15440 (SJN48_15490) pilT 3259123..3260160 (+) 1038 WP_002121749.1 type IV pilus twitching motility protein PilT Machinery gene
  SJN48_RS15445 (SJN48_15495) pilU 3260180..3261298 (+) 1119 WP_005070855.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SJN48_RS15450 (SJN48_15500) fur 3261368..3261805 (-) 438 WP_002121758.1 ferric iron uptake transcriptional regulator -
  SJN48_RS15455 (SJN48_15505) omlA 3261917..3262315 (+) 399 WP_002121740.1 outer membrane protein assembly factor BamE -
  SJN48_RS15460 (SJN48_15510) - 3262380..3262694 (-) 315 WP_130121014.1 RnfH family protein -
  SJN48_RS15465 (SJN48_15515) - 3262694..3263761 (-) 1068 WP_130121015.1 hypothetical protein -
  SJN48_RS15470 (SJN48_15520) - 3263915..3264370 (-) 456 WP_002121698.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38389.97 Da        Isoelectric Point: 6.7269

>NTDB_id=908118 SJN48_RS15440 WP_002121749.1 3259123..3260160(+) (pilT) [Acinetobacter pittii strain WWTP]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALDHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KNLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=908118 SJN48_RS15440 WP_002121749.1 3259123..3260160(+) (pilT) [Acinetobacter pittii strain WWTP]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACACCTATCTGCTGGCATGCCACCAAT
GATTCGCGTGGATGGTGAAGTCCGTCGCATTAACTTACCTGCTCTAGATCATAAAGATGTTCATCGTCTGGTCTACGACA
TCATGAATGATAAACAGCGTCGTGACTACGAAGAAAAACTCGAAACCGACTTTTCTTTTGAAGTACCCAACGTTGCGCGT
TTCCGTGTAAACGCATTTAACCAAAACCGTGGTGCGGGTGCAGTATTTCGTACCATTCCGTCCAAAGTACTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGATATTTGTGACTATCCACGCGGTATTGTATTAGTTACGGGTCCAACAGGTT
CTGGTAAATCGACCACACTTGCAGCGATGATGGACTACATCAACGAAAACCGTTATGACCACATCCTAACGGTTGAAGAC
CCTATCGAATTTGTACACCAATCTAAGAAATGTCTCATCAACCAACGTGAAGTGCATCGTGATACACACGGCTTTAATGA
GGCACTACGTTCAGCACTGCGTGAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAGACTATTCGTTTAG
CACTTACTGCTGCCGAAACAGGTCATTTGGTGTTTGGCACACTGCATACCACTTCTGCTGCGAAAACCATCGACCGTGTA
ATTGACGTATTCCCAGCCGAAGAAAAAGACATGGTTCGTGCCATGCTCTCTGAATCATTGCAGGCTGTAATTTCACAAAC
CCTGCTTAAAAAGAATGGCGGCGGCCGTGTGGCAGCTCATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAATAAAGTCGCGCAAATGTACTCAGCTATTCAGACTGGCGCAAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAGAATTTGGTGTCTCGCGGTGTGATCAGCCCGCAAACCGCTCGTACTGCTGCAAAACAGCCAGAATCATTTCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009I411

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

98.841

100

0.988
  pilT Acinetobacter baumannii strain A118

98.841

100

0.988
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391