Detailed information NTG0908119

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SJN48_RS15445
• Genome accession: NZ_CP139212
• Coordinates: 3260180..3261298 (+)
• Length: 372 a.a.
• NCBI ID: WP_005070855.1
• Uniprot ID: A0A013SVN8
• Organism: Acinetobacter pittii strain WWTP
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3255180..3266298

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SJN48_RS15435 (SJN48_15485)	yggS	3258304..3258996 (-)	693	WP_130121013.1	YggS family pyridoxal phosphate-dependent enzyme	-
SJN48_RS15440 (SJN48_15490)	pilT	3259123..3260160 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
SJN48_RS15445 (SJN48_15495)	pilU	3260180..3261298 (+)	1119	WP_005070855.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SJN48_RS15450 (SJN48_15500)	fur	3261368..3261805 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
SJN48_RS15455 (SJN48_15505)	omlA	3261917..3262315 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
SJN48_RS15460 (SJN48_15510)	-	3262380..3262694 (-)	315	WP_130121014.1	RnfH family protein	-
SJN48_RS15465 (SJN48_15515)	-	3262694..3263761 (-)	1068	WP_130121015.1	hypothetical protein	-
SJN48_RS15470 (SJN48_15520)	-	3263915..3264370 (-)	456	WP_002121698.1	bacteriohemerythrin	-
SJN48_RS15475 (SJN48_15525)	-	3264622..3265470 (-)	849	WP_014205980.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41931.15 Da
• Isoelectric Point: 6.6404

>NTDB_id=908119 SJN48_RS15445 WP_005070855.1 3260180..3261298(+) (pilU) [Acinetobacter pittii strain WWTP]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=908119 SJN48_RS15445 WP_005070855.1 3260180..3261298(+) (pilU) [Acinetobacter pittii strain WWTP]
ATGGATTTTAACGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGACAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTGCTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGATGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACGTTGCGACAAGCACCCGATGTGATCTTAATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCAACGATGCATGCAAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCCCCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCATTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A013SVN8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	53.784	99.462	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363