Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SK095_RS13445 Genome accession   NZ_CP139195
Coordinates   2868991..2870025 (-) Length   344 a.a.
NCBI ID   WP_136490455.1    Uniprot ID   A0A8H8XQB0
Organism   Pseudomonas sp. AN-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2863991..2875025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK095_RS13420 (SK095_13420) pyrR 2864438..2864953 (+) 516 WP_136490460.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  SK095_RS13425 (SK095_13425) - 2864965..2865969 (+) 1005 WP_136490459.1 aspartate carbamoyltransferase catalytic subunit -
  SK095_RS13430 (SK095_13430) - 2865966..2867237 (+) 1272 WP_136490458.1 dihydroorotase -
  SK095_RS13435 (SK095_13435) - 2867298..2867702 (-) 405 WP_136490457.1 NINE protein -
  SK095_RS13440 (SK095_13440) pilU 2867793..2868941 (-) 1149 WP_136490456.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SK095_RS13445 (SK095_13445) pilT 2868991..2870025 (-) 1035 WP_136490455.1 type IV pilus twitching motility protein PilT Machinery gene
  SK095_RS13450 (SK095_13450) - 2870097..2870804 (+) 708 WP_320546571.1 YggS family pyridoxal phosphate-dependent enzyme -
  SK095_RS13455 (SK095_13455) proC 2870840..2871658 (+) 819 WP_320546572.1 pyrroline-5-carboxylate reductase -
  SK095_RS13460 (SK095_13460) - 2871668..2872261 (+) 594 WP_320546573.1 YggT family protein -
  SK095_RS13465 (SK095_13465) - 2872261..2872566 (+) 306 WP_320546574.1 DUF167 domain-containing protein -
  SK095_RS13470 (SK095_13470) metX 2872697..2873836 (+) 1140 WP_320546575.1 homoserine O-succinyltransferase MetX -
  SK095_RS13475 (SK095_13475) metW 2873852..2874442 (+) 591 WP_320546576.1 methionine biosynthesis protein MetW -
  SK095_RS13480 (SK095_13480) - 2874457..2874888 (+) 432 WP_136490448.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37848.66 Da        Isoelectric Point: 6.8362

>NTDB_id=907810 SK095_RS13445 WP_136490455.1 2868991..2870025(-) (pilT) [Pseudomonas sp. AN-1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSRVLSMEDLGMGEVFKKVSDVPRGLVLVTGPTGSGKSTTLAAMLDYINNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDACL
KTLVTKGLVSREAAREKAKAPESI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=907810 SK095_RS13445 WP_136490455.1 2868991..2870025(-) (pilT) [Pseudomonas sp. AN-1]
ATGGATATTACCGAGCTGCTGGCCTTCAGCGCCAAGCAGGGCGCATCCGACCTGCACCTCTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGTCGCATCAACCTGCCGCCGATGGAGCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAGTTCCTCGAGACCGACTTCTCCTTCGAGGTGCCCGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCAGTGTTCCGGACCATTCCCTCGCGGGTGCTGAGCATGGA
AGACCTCGGCATGGGCGAGGTGTTCAAGAAGGTCTCCGACGTACCGCGCGGCCTGGTGCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGACTACATCAACAACACCAAGTACCACCACATCCTCACCATCGAGGAT
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTCGGCTTCGCCGA
GGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGCCGAGGAGAAGTCCATGGTCCGCTCCATGCTCTCCGAGTCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGGTCGGCGGCGGCCGGGTGGCCGCCCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGCGCGCTGGGCATGCAGACCCTCGACGCCTGTCTG
AAGACGCTGGTGACCAAGGGGCTGGTCAGCCGCGAGGCCGCCCGCGAGAAGGCCAAGGCACCGGAAAGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

93.605

100

0.936
  pilT Pseudomonas stutzeri DSM 10701

92.733

100

0.927
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

76.627

98.256

0.753
  pilT Legionella pneumophila strain ERS1305867

76.627

98.256

0.753
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Neisseria meningitidis 8013

69.275

100

0.695
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.187

99.709

0.52
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39