Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SK095_RS13440 Genome accession   NZ_CP139195
Coordinates   2867793..2868941 (-) Length   382 a.a.
NCBI ID   WP_136490456.1    Uniprot ID   A0A8H8XPQ3
Organism   Pseudomonas sp. AN-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2862793..2873941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK095_RS13410 (SK095_13410) - 2863423..2863989 (+) 567 WP_320546570.1 YqgE/AlgH family protein -
  SK095_RS13415 (SK095_13415) ruvX 2863986..2864420 (+) 435 WP_136490461.1 Holliday junction resolvase RuvX -
  SK095_RS13420 (SK095_13420) pyrR 2864438..2864953 (+) 516 WP_136490460.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  SK095_RS13425 (SK095_13425) - 2864965..2865969 (+) 1005 WP_136490459.1 aspartate carbamoyltransferase catalytic subunit -
  SK095_RS13430 (SK095_13430) - 2865966..2867237 (+) 1272 WP_136490458.1 dihydroorotase -
  SK095_RS13435 (SK095_13435) - 2867298..2867702 (-) 405 WP_136490457.1 NINE protein -
  SK095_RS13440 (SK095_13440) pilU 2867793..2868941 (-) 1149 WP_136490456.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SK095_RS13445 (SK095_13445) pilT 2868991..2870025 (-) 1035 WP_136490455.1 type IV pilus twitching motility protein PilT Machinery gene
  SK095_RS13450 (SK095_13450) - 2870097..2870804 (+) 708 WP_320546571.1 YggS family pyridoxal phosphate-dependent enzyme -
  SK095_RS13455 (SK095_13455) proC 2870840..2871658 (+) 819 WP_320546572.1 pyrroline-5-carboxylate reductase -
  SK095_RS13460 (SK095_13460) - 2871668..2872261 (+) 594 WP_320546573.1 YggT family protein -
  SK095_RS13465 (SK095_13465) - 2872261..2872566 (+) 306 WP_320546574.1 DUF167 domain-containing protein -
  SK095_RS13470 (SK095_13470) metX 2872697..2873836 (+) 1140 WP_320546575.1 homoserine O-succinyltransferase MetX -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42699.91 Da        Isoelectric Point: 6.7792

>NTDB_id=907809 SK095_RS13440 WP_136490456.1 2867793..2868941(-) (pilU) [Pseudomonas sp. AN-1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRILPVTKTAMSPEQTRETVLGVMNEHQRREFAGNHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETQIPTFDELQLPEVLKQLSMTKRGLVLFVGATGAGKSTSLAAMIGYRNKNSQGHIISIED
PIEFIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
IHFFPPDRHEQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKNLMKRSTEQGMQTFDQA
LYQLYSNAEITYEDALAHADSANDLRLMIKLGSETDAEHLSNLTRSLSLEGSDEPQSGLRRR

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=907809 SK095_RS13440 WP_136490456.1 2867793..2868941(-) (pilU) [Pseudomonas sp. AN-1]
ATGGAATTCGAGAAACTGCTGCGCCTGATGGTCGAGAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTGCCGCCGTC
GATGAAGGTCAACGGGCGCATCCTGCCGGTGACCAAGACCGCCATGTCGCCGGAGCAGACCCGCGAGACGGTGCTGGGCG
TGATGAACGAGCACCAGCGCCGCGAGTTCGCCGGCAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACGCAGATCCCCACCTTCGA
CGAACTCCAGCTGCCCGAGGTGCTCAAGCAGCTGTCGATGACCAAGCGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGCG
CGGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCGCAGGGGCACATCATCTCCATCGAGGAC
CCCATCGAGTTCATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGACCATGGACTACG
CGGTGGCCTTCGCCGAGACCGGTCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTCGACCGCATC
ATCCACTTCTTCCCGCCGGACCGTCACGAGCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCCGATGGCAAGGGGCGCCGCGCGGTGATCGAGGTGCTGATCAACACGCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGAACCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTCTACCAGCTGTACAGCAATGCCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGCTCGGAGACCGATGCCGAGCACCTGAGCAACCTGACCCGCAGTCTCAGCCTGGAAGGCAGCGACG
AGCCGCAGAGCGGCCTGCGCCGGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

87.433

97.906

0.856
  pilU Acinetobacter baylyi ADP1

62.334

98.691

0.615
  pilU Vibrio cholerae strain A1552

60.571

91.623

0.555
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.773

88.743

0.38