Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SK090_RS18365 Genome accession   NZ_CP139189
Coordinates   4074008..4075042 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain FS12001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4069008..4080042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK090_RS18335 glnK 4069588..4069926 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  SK090_RS18340 arfB 4070128..4070544 (+) 417 WP_139432403.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  SK090_RS18345 - 4070577..4071068 (+) 492 WP_201953722.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  SK090_RS18350 - 4071429..4071854 (-) 426 WP_005340360.1 hypothetical protein -
  SK090_RS18355 yaaA 4072049..4072822 (-) 774 WP_320545425.1 peroxide stress protein YaaA -
  SK090_RS18360 pilU 4072870..4073979 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  SK090_RS18365 pilT 4074008..4075042 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  SK090_RS18370 - 4075082..4075783 (+) 702 WP_100654584.1 YggS family pyridoxal phosphate-dependent enzyme -
  SK090_RS18375 proC 4075926..4076750 (+) 825 WP_100654585.1 pyrroline-5-carboxylate reductase -
  SK090_RS18380 - 4076762..4077313 (+) 552 WP_047436998.1 YggT family protein -
  SK090_RS18385 yggU 4077313..4077615 (+) 303 WP_021230942.1 DUF167 family protein YggU -
  SK090_RS18390 - 4077659..4078078 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -
  SK090_RS18395 - 4078169..4079365 (-) 1197 WP_160881766.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=907693 SK090_RS18365 WP_005340354.1 4074008..4075042(-) (pilT) [Aeromonas veronii strain FS12001]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=907693 SK090_RS18365 WP_005340354.1 4074008..4075042(-) (pilT) [Aeromonas veronii strain FS12001]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGTTCGGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAAATCGCCGAGTTCCCGCGGGGTCTGGTACTGGTGACCGGCCCGACCGGTT
CCGGTAAGTCGACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCATATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCACCGTGATACCAAGAGCTTCAGCAA
CGCTCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGATCTAGAGACCATTCGTTTGG
CCATGACCGCCGCCGAAACTGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGTGCGGAGAAGGACATGGTGCGCTCCATGCTCTCTGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTCCGTAACTTGATCC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAATTGGTGAGCCGCGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCCGTGGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407