Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SK090_RS18360 Genome accession   NZ_CP139189
Coordinates   4072870..4073979 (-) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain FS12001     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4067870..4078979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK090_RS18330 - 4068323..4069330 (-) 1008 WP_005357960.1 Fe(3+) ABC transporter substrate-binding protein -
  SK090_RS18335 glnK 4069588..4069926 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  SK090_RS18340 arfB 4070128..4070544 (+) 417 WP_139432403.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  SK090_RS18345 - 4070577..4071068 (+) 492 WP_201953722.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  SK090_RS18350 - 4071429..4071854 (-) 426 WP_005340360.1 hypothetical protein -
  SK090_RS18355 yaaA 4072049..4072822 (-) 774 WP_320545425.1 peroxide stress protein YaaA -
  SK090_RS18360 pilU 4072870..4073979 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  SK090_RS18365 pilT 4074008..4075042 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  SK090_RS18370 - 4075082..4075783 (+) 702 WP_100654584.1 YggS family pyridoxal phosphate-dependent enzyme -
  SK090_RS18375 proC 4075926..4076750 (+) 825 WP_100654585.1 pyrroline-5-carboxylate reductase -
  SK090_RS18380 - 4076762..4077313 (+) 552 WP_047436998.1 YggT family protein -
  SK090_RS18385 yggU 4077313..4077615 (+) 303 WP_021230942.1 DUF167 family protein YggU -
  SK090_RS18390 - 4077659..4078078 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=907692 SK090_RS18360 WP_005340356.1 4072870..4073979(-) (pilU) [Aeromonas veronii strain FS12001]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=907692 SK090_RS18360 WP_005340356.1 4072870..4073979(-) (pilU) [Aeromonas veronii strain FS12001]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAAGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAGGCGCTTGGTCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAATTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACTCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCGACCGAGTTGGGTATGCAGACCTTCGATCAGGCG
CTTTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCCCACGCCGACTCGGCCAACGACCTGCGATT
GCTGATCAAGCTCTCCGGTCGCGAACGGCTCGGCACCGGTACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36