Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB68_RS08570 Genome accession   NZ_CP138973
Coordinates   1750082..1751158 (+) Length   358 a.a.
NCBI ID   WP_063399885.1    Uniprot ID   -
Organism   Prochlorococcus sp. MIT 1323     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1745082..1756158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB68_RS08550 - 1746020..1746265 (-) 246 WP_063399876.1 sulfurtransferase TusA family protein -
  SIB68_RS08555 dnaJ 1746262..1747398 (-) 1137 WP_063399879.1 molecular chaperone DnaJ -
  SIB68_RS08560 grpE 1747443..1748156 (-) 714 WP_063399881.1 nucleotide exchange factor GrpE -
  SIB68_RS08565 pilB/pilB1 1748155..1750071 (+) 1917 WP_063399883.1 GspE/PulE family protein Machinery gene
  SIB68_RS08570 pilT 1750082..1751158 (+) 1077 WP_063399885.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB68_RS08575 - 1751175..1752455 (+) 1281 WP_063399887.1 type II secretion system F family protein -
  SIB68_RS08580 - 1752430..1753752 (-) 1323 WP_063399889.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB68_RS08585 msrB 1753679..1754185 (-) 507 WP_063400032.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB68_RS08590 dusA 1754255..1755259 (+) 1005 WP_063399891.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB68_RS08595 - 1755269..1755844 (-) 576 WP_413681826.1 RNA recognition motif domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39408.87 Da        Isoelectric Point: 5.9277

>NTDB_id=906399 SIB68_RS08570 WP_063399885.1 1750082..1751158(+) (pilT) [Prochlorococcus sp. MIT 1323]
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELRPMTNTPLSEETCNKLIFSMLNNSQRKTLEQIWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIESLNLPSVVIETSKRPRGLVLVTGPTGSGKTTTLASLLDHINHTRSEHILTI
EDPIEFVYKSDLSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLIFGTLHTNSAAQTVD
RMVDVFPPNQQTQIRVQLSGSLLAIFSQTLCRRQNPQPEQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLTKQGEISRSEAIAKASKPSELEQLLNDG

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=906399 SIB68_RS08570 WP_063399885.1 1750082..1751158(+) (pilT) [Prochlorococcus sp. MIT 1323]
ATGGATCTAATGATCGAGGACCTGATGGAACAGCTGGTGCAAGGTGGCGGCAGTGATCTGCACATTGCTACCGGCCAGCC
CCCGTATGGACGATTCAGCGGTGAATTAAGGCCAATGACCAATACCCCCCTATCCGAAGAAACATGCAACAAGCTGATTT
TCTCAATGCTCAACAACAGTCAGAGAAAGACTCTTGAACAAATCTGGGAGCTTGACTGTGCCTATGGGCTAAAGGGTGTG
GCACGCTTTCGCGTGAATGTCTATCGACAGAAGGGAAGTTACGCCGCCTGTCTACGTGCTCTTGGCAGCACTATCCCGAG
TATTGAAAGTCTCAACCTGCCATCAGTAGTCATAGAAACCAGCAAACGACCAAGGGGATTAGTGCTAGTAACAGGACCAA
CCGGATCAGGCAAAACAACAACCCTTGCTTCCCTGCTGGACCACATCAATCACACCAGAAGCGAACACATCCTCACGATC
GAAGATCCAATCGAATTCGTATACAAAAGCGATCTCAGCCTTGTGCACCAGCGACAGCTCAATGAAGACACACGCAGTTT
TGGCAATGCCCTGCGTGCCGCACTACGCGAAGATCCAGATGTGATCCTCGTGGGCGAAATGCGCGACCTGGAAACCATTC
AACTGGCCATCAGCGCCGCCGAAACCGGTCACCTCATATTCGGCACCCTGCACACCAACTCGGCGGCACAAACGGTCGAT
CGCATGGTTGATGTTTTCCCGCCAAACCAGCAAACCCAAATCAGAGTTCAACTCTCAGGAAGCCTGCTAGCCATTTTTTC
TCAAACCCTCTGCCGTCGCCAAAATCCACAACCAGAACAATTTGGGCGGGTCATGGCTCAAGAAATCTTGATCAACACCC
CAGCCACAGCAAACCTGATCAGAGAAGGCAAAACCGCACAGCTCTACTCCCAAATTCAAACCGGAGGAGAACTAGGGATG
CAAACACTAGAAAAAGCCCTTGCGAATCTAACTAAACAGGGTGAAATTAGCCGCTCTGAAGCCATCGCCAAAGCCAGTAA
ACCCAGCGAACTGGAGCAACTCCTCAACGATGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.616

96.089

0.506
  pilT Pseudomonas stutzeri DSM 10701

52.035

96.089

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.575

98.603

0.489
  pilT Legionella pneumophila strain Lp02

51.039

94.134

0.48
  pilT Legionella pneumophila strain ERS1305867

51.039

94.134

0.48
  pilT Acinetobacter baylyi ADP1

50

96.089

0.48
  pilT Neisseria gonorrhoeae MS11

49.425

97.207

0.48
  pilT Neisseria meningitidis 8013

49.854

95.81

0.478
  pilT Acinetobacter nosocomialis M2

48.991

96.927

0.475
  pilT Acinetobacter baumannii D1279779

48.991

96.927

0.475
  pilT Acinetobacter baumannii strain A118

48.991

96.927

0.475
  pilT Vibrio cholerae strain A1552

47.941

94.972

0.455
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.941

94.972

0.455
  pilU Pseudomonas stutzeri DSM 10701

40.395

98.883

0.399
  pilU Acinetobacter baylyi ADP1

37.356

97.207

0.363
  pilU Vibrio cholerae strain A1552

37.941

94.972

0.36