Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SIB68_RS08565 Genome accession   NZ_CP138973
Coordinates   1748155..1750071 (+) Length   638 a.a.
NCBI ID   WP_063399883.1    Uniprot ID   -
Organism   Prochlorococcus sp. MIT 1323     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1743155..1755071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB68_RS08535 murB 1743822..1744745 (+) 924 WP_063399873.1 UDP-N-acetylmuramate dehydrogenase -
  SIB68_RS08540 - 1744770..1745111 (+) 342 WP_011824714.1 YbaB/EbfC family nucleoid-associated protein -
  SIB68_RS08545 rsgA 1745068..1746033 (-) 966 WP_063399874.1 ribosome small subunit-dependent GTPase A -
  SIB68_RS08550 - 1746020..1746265 (-) 246 WP_063399876.1 sulfurtransferase TusA family protein -
  SIB68_RS08555 dnaJ 1746262..1747398 (-) 1137 WP_063399879.1 molecular chaperone DnaJ -
  SIB68_RS08560 grpE 1747443..1748156 (-) 714 WP_063399881.1 nucleotide exchange factor GrpE -
  SIB68_RS08565 pilB/pilB1 1748155..1750071 (+) 1917 WP_063399883.1 GspE/PulE family protein Machinery gene
  SIB68_RS08570 pilT 1750082..1751158 (+) 1077 WP_063399885.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB68_RS08575 - 1751175..1752455 (+) 1281 WP_063399887.1 type II secretion system F family protein -
  SIB68_RS08580 - 1752430..1753752 (-) 1323 WP_063399889.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB68_RS08585 msrB 1753679..1754185 (-) 507 WP_063400032.1 peptide-methionine (R)-S-oxide reductase MsrB -

Sequence

Protein


Download         Length: 638 a.a.        Molecular weight: 70412.34 Da        Isoelectric Point: 6.4567

>NTDB_id=906398 SIB68_RS08565 WP_063399883.1 1748155..1750071(+) (pilB/pilB1) [Prochlorococcus sp. MIT 1323]
MLTVCKFTSRHVDGSSTPPTSGGSPHLQFHFFFVTQGRPIPKATNSIQQRLELELLLQVSVLSHEELVVGVEVMANHTTL
DISTWQQFQALPINMHNQHLVVAISDQCNEQTKNQLISVLQSQGFSTEFRLALASDISELLAPMRSEQHVESASKSKATK
PIAQTPTSLLAGFSAEGVLEEDPEEQARLASYVEDLESSLMDSDSSPVINLVDRILLEALQTSASDVHVEPQQDGLQIRF
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSLPSRYGEKICLRLLDSSAPQL
GLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFD
FSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPNCQGSGYKGRVGVYEVLRMNEELATAVAKGATTD
LVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDSGLESERRARALSTLTCNVCGAGLQDGWLECPYCLTPRQ

Nucleotide


Download         Length: 1917 bp        

>NTDB_id=906398 SIB68_RS08565 WP_063399883.1 1748155..1750071(+) (pilB/pilB1) [Prochlorococcus sp. MIT 1323]
ATGCTGACCGTTTGCAAATTCACGTCTAGACATGTTGATGGGAGTAGCACACCTCCCACAAGCGGCGGAAGCCCGCACCT
CCAATTTCATTTCTTCTTCGTGACCCAGGGCCGACCAATCCCCAAAGCAACCAACAGCATCCAGCAGCGACTGGAACTGG
AACTGCTCCTACAAGTCAGTGTGCTTAGCCACGAAGAACTTGTTGTCGGAGTGGAGGTAATGGCCAACCACACCACTCTT
GACATCTCAACGTGGCAACAATTCCAGGCCCTGCCAATCAACATGCACAACCAACATTTGGTTGTAGCCATCTCGGACCA
ATGCAATGAGCAAACCAAAAATCAACTGATCTCAGTACTTCAGTCCCAAGGCTTCAGCACAGAATTCCGTCTCGCGCTGG
CATCCGACATCAGCGAGCTGCTTGCACCAATGAGATCTGAGCAGCACGTTGAATCTGCATCCAAGTCAAAAGCAACAAAG
CCAATTGCACAAACACCAACCTCCCTGCTGGCAGGATTTAGTGCCGAAGGCGTGCTCGAAGAAGATCCTGAAGAACAAGC
CAGACTTGCTAGTTACGTAGAGGATTTGGAGTCCAGCTTGATGGACTCAGACAGCTCACCAGTCATCAACCTGGTGGACC
GCATACTGCTAGAAGCACTGCAAACATCAGCCAGCGACGTACACGTTGAGCCGCAACAAGACGGACTACAGATCCGCTTC
CGTCAAGACGGTGTCCTGCAGCGCTATATCGAACCCCTCCCGAGCCGGTTGATCCCTGCTGTTACCTCACGCTTCAAGAT
CATGGCCGACCTAGACATCGCAGAACGGCGCATGGCTCAGGACGGTCGCATTCGTCGCACATATCGCAATCGTATGGTCG
ATTTCAGGGTTAATAGCCTGCCAAGCCGTTACGGGGAAAAAATCTGTCTGCGACTCCTTGACAGCAGTGCTCCACAACTC
GGGCTAGACAAACTGATCAGCAACCCAAGTGCTCTCTCTCTCGTACGCAACTTGGGCTCTAAGCCCTTCGGCATGATCCT
TGTGACAGGTCCAACAGGATCAGGCAAATCAACGACCCTTTACTCCCTACTGGCTGAACGCAACGAGCCTGGTATCAACA
TTTCCACCGTAGAAGATCCCATCGAATACACCCTCCCTGGGATTACTCAATGTCAAGTGAACAGGGAAAAAGGCTTCGAT
TTCAGTACTGCACTGAGAGCCTTCATGCGTCAAGACCCAGACGTTCTGCTGGTCGGTGAAACCCGTGACCTAGAAACCGC
AAAAACCGCCATCGAAGCCGCACTGACTGGTCATCTGGTGCTAACCACACTGCATTGCAATGACGCATCAAGTGCGATCG
CCCGCCTCAACGAGATGGGCGTGGAACCATTCATGGTGAGCGCCTCGTTGATTGGCATCGTCTCTCAACGACTTCTACGA
AGAGTGTGCCGTTCTTGTCGTAAGTCCTATCACCCAACTGAAAAAGAACTGGGGCGATTCGGATTGATGGCCCATACAGA
AACTGGCGTGACCTTTTTCAAGGCCCACCACCACGGTCAAGAAAAACAGCCGTGCCCCAACTGTCAAGGCAGCGGCTACA
AGGGCCGAGTTGGTGTCTATGAAGTACTGCGCATGAACGAAGAGCTCGCTACAGCCGTCGCCAAAGGTGCCACTACTGAT
TTAGTAAGACGTCTGGCGCTAGAAGCTGGTATGAAAACTCTTTTGGGCTACAGCCTTGACCTGGTGAGAGAAGGCCACAC
CACCCTTGAGGAAGTGGGCCGAATGATCCTCACCGATTCCGGATTGGAATCTGAGCGCCGCGCAAGAGCCCTTAGCACTC
TCACCTGCAACGTCTGCGGGGCAGGCCTCCAAGACGGCTGGCTGGAATGTCCCTACTGTCTAACCCCTCGCCAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-148)

Type II/IV secretion system protein (T2SSE)

(215-481)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

53.63

94.984

0.509