Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB72_RS04285 Genome accession   NZ_CP138971
Coordinates   823943..825019 (-) Length   358 a.a.
NCBI ID   WP_413744717.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9451     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 818943..830019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB72_RS04260 - 819252..819776 (+) 525 WP_413744712.1 RNA recognition motif domain-containing protein -
  SIB72_RS04265 dusA 819786..820790 (-) 1005 WP_413744713.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB72_RS04270 msrB 820862..821374 (+) 513 WP_413744714.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB72_RS04275 - 821301..822596 (+) 1296 WP_413744715.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB72_RS04280 - 822634..823932 (-) 1299 WP_413744716.1 type II secretion system F family protein -
  SIB72_RS04285 pilT 823943..825019 (-) 1077 WP_413744717.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB72_RS04290 pilB/pilB1 825030..826766 (-) 1737 WP_413744718.1 GspE/PulE family protein Machinery gene
  SIB72_RS04295 grpE 826876..827613 (+) 738 WP_413744719.1 nucleotide exchange factor GrpE -
  SIB72_RS04300 dnaJ 827659..828792 (+) 1134 WP_115119293.1 molecular chaperone DnaJ -
  SIB72_RS04305 - 828795..829037 (+) 243 WP_413744720.1 sulfurtransferase TusA family protein -
  SIB72_RS04310 rsgA 829030..829929 (+) 900 WP_413744721.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39204.75 Da        Isoelectric Point: 6.3061

>NTDB_id=906368 SIB72_RS04285 WP_413744717.1 823943..825019(-) (pilT) [Synechococcus sp. MIT S9451]
MDLMIEDLMEQLVKAEGSDLHLACGQPPYGRFSGELRPLSDTPLSEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSSIPSIEALNLPPVVVETSARPRGLVLVTGPTGSGKTTTLAALLDHINHTRSEHILTI
EDPIEFVYRSDRSMVHQRQLHEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLMAVFSQTLCRRDKPQPGEFGRVMAQEILINTPAIANLIREGKTAQLYSQLQTGGELGM
QTLERALANLVQTGAISQAEAMTKAGKPGELNRLLGNS

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=906368 SIB72_RS04285 WP_413744717.1 823943..825019(-) (pilT) [Synechococcus sp. MIT S9451]
ATGGATCTGATGATCGAAGACCTGATGGAGCAACTGGTCAAGGCCGAAGGCAGTGATCTGCATCTCGCCTGCGGCCAACC
ACCGTACGGCCGTTTCAGCGGCGAACTGCGCCCCCTCTCCGACACACCGCTGAGTGAGGAGAGCTGCAACCGCCTGATCT
TCTCAATGCTCAATAACAGCCAGCGCAAGACGCTGGAACAGACCTGGGAGCTCGATTGTGCCTACGGCCTCAAAGGCGTG
GCACGCTTCCGCGTGAACGTCTATCGCCAGAAGGGCAGCTATGCCGCCTGCCTCAGGGCGCTCGGCAGTTCAATTCCCAG
TATCGAGGCACTCAACCTGCCCCCAGTGGTGGTGGAAACCTCTGCCCGCCCCCGCGGCCTTGTGCTGGTGACGGGACCGA
CCGGATCCGGCAAAACCACCACATTGGCGGCACTGCTCGACCACATCAACCACACCCGCAGCGAGCACATCCTCACGATC
GAGGATCCGATCGAATTCGTGTACCGCAGTGACCGCAGCATGGTGCACCAGCGCCAGTTGCATGAAGACACTCGCAGCTT
TGCCAATGCCCTACGGGCTGCGCTGCGCGAAGACCCCGACGTGATTCTGGTGGGTGAGATGCGCGATCTGGAAACGATTC
AACTCGCCATCAGTGCAGCCGAAACCGGTCACTTGGTCTTCGGCACCCTGCACACAAGCTCAGCCGCCCAGACCGTCGAT
CGCATGGTGGATGTGTTCCCACCTGGCCAGCAGACCCAAATCCGTGTGCAGCTATCGGGAAGTCTGATGGCCGTGTTCTC
CCAAACCCTCTGCCGTCGCGACAAGCCGCAGCCCGGCGAATTCGGACGGGTGATGGCTCAAGAGATCTTGATCAACACCC
CGGCAATCGCAAACCTGATCCGTGAAGGCAAAACGGCTCAGCTCTATTCCCAACTTCAGACCGGGGGTGAACTGGGCATG
CAGACCCTGGAACGGGCCCTTGCCAATCTCGTCCAAACCGGAGCAATCAGCCAGGCAGAAGCCATGACCAAGGCCGGGAA
ACCAGGCGAACTGAATCGTCTCCTAGGGAACAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.779

96.089

0.517
  pilT Pseudomonas stutzeri DSM 10701

52.907

96.089

0.508
  pilT Neisseria meningitidis 8013

52.187

95.81

0.5
  pilT Neisseria gonorrhoeae MS11

51.895

95.81

0.497
  pilT Acinetobacter nosocomialis M2

51.453

96.089

0.494
  pilT Acinetobacter baumannii D1279779

51.453

96.089

0.494
  pilT Acinetobacter baumannii strain A118

51.453

96.089

0.494
  pilT Legionella pneumophila strain ERS1305867

52.226

94.134

0.492
  pilT Legionella pneumophila strain Lp02

52.226

94.134

0.492
  pilT Acinetobacter baylyi ADP1

50.291

96.089

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.305

98.883

0.478
  pilT Vibrio cholerae strain A1552

50.294

94.972

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

94.972

0.478
  pilU Pseudomonas stutzeri DSM 10701

39.031

98.045

0.383
  pilU Vibrio cholerae strain A1552

38.617

96.927

0.374
  pilU Acinetobacter baylyi ADP1

38.218

97.207

0.372