Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SIB72_RS04290 Genome accession   NZ_CP138971
Coordinates   825030..826766 (-) Length   578 a.a.
NCBI ID   WP_413744718.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9451     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 820030..831766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB72_RS04270 msrB 820862..821374 (+) 513 WP_413744714.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB72_RS04275 - 821301..822596 (+) 1296 WP_413744715.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB72_RS04280 - 822634..823932 (-) 1299 WP_413744716.1 type II secretion system F family protein -
  SIB72_RS04285 pilT 823943..825019 (-) 1077 WP_413744717.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB72_RS04290 pilB/pilB1 825030..826766 (-) 1737 WP_413744718.1 GspE/PulE family protein Machinery gene
  SIB72_RS04295 grpE 826876..827613 (+) 738 WP_413744719.1 nucleotide exchange factor GrpE -
  SIB72_RS04300 dnaJ 827659..828792 (+) 1134 WP_115119293.1 molecular chaperone DnaJ -
  SIB72_RS04305 - 828795..829037 (+) 243 WP_413744720.1 sulfurtransferase TusA family protein -
  SIB72_RS04310 rsgA 829030..829929 (+) 900 WP_413744721.1 ribosome small subunit-dependent GTPase A -
  SIB72_RS04315 - 829904..830245 (-) 342 WP_115019006.1 YbaB/EbfC family nucleoid-associated protein -
  SIB72_RS04320 murB 830278..831201 (-) 924 WP_413744722.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63920.10 Da        Isoelectric Point: 5.0892

>NTDB_id=906369 SIB72_RS04290 WP_413744718.1 825030..826766(-) (pilB/pilB1) [Synechococcus sp. MIT S9451]
MRDQQRFVLELLLQEPVPERLDQDSHQQLLTRLEAKGLVPNKHPRLAAALIALQSQEANQTTAALAESNQAEADINLPQD
PGEETLDDPPESQEESEAPINLDSLLSGFNSDGVLEEEPEEAARLGSRHEDLEQTFRDTNASPVVNLVNRILLQALQLGA
SDIHVEPQQIGLQVRFRQDGVLQSYSEPLPSRLVPAVTSRFKIMADLDIAERRQAQDGRIRRRFRQRSVDFRVSSLPSRF
GEKIVLRLLDSSSTQLGLDKLISNPEALELVRSLGSKPFGMILVTGPTGSGKSTTLYSLLAERNDPGINISTVEDPIEYT
LPGITQCQVNREKGFDFSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLGEMGVEPF
MVSASLIGIVSQRLLRRVCSHCRESYQPDATELGRFGLMTHHETGVRFYRAHHHDGNRDHSGDPCPHCQGSGYRGRIGVY
EVLRMNEQLATAVANGATTDVLRRLALESGMKTLLGYSLELVRQGQTTLEEVGRMILTDSGLESERRARALSTVTCERCG
GGLQEGWLECPYCLTPRQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=906369 SIB72_RS04290 WP_413744718.1 825030..826766(-) (pilB/pilB1) [Synechococcus sp. MIT S9451]
GTGAGAGACCAGCAGCGTTTTGTCCTGGAGCTGCTGTTGCAGGAACCGGTCCCGGAACGACTGGATCAAGACAGCCATCA
GCAACTGCTGACAAGACTTGAAGCAAAAGGACTTGTCCCCAACAAGCATCCACGCCTGGCCGCAGCCCTGATCGCCCTCC
AGAGCCAGGAGGCCAACCAGACCACTGCTGCTTTGGCGGAGTCAAATCAAGCAGAAGCAGACATCAACCTGCCGCAGGAT
CCTGGAGAAGAAACACTGGATGACCCCCCAGAAAGCCAGGAAGAGAGCGAGGCACCCATCAACCTCGATTCACTGCTCTC
TGGCTTCAACAGCGACGGGGTCCTGGAAGAAGAGCCCGAAGAAGCAGCCCGACTGGGCTCAAGACACGAAGACCTCGAAC
AAACCTTCCGAGACACGAACGCCTCACCAGTGGTGAATCTGGTGAACCGCATCCTGCTTCAGGCCCTGCAACTTGGGGCA
AGCGACATTCATGTGGAACCCCAACAAATCGGCCTCCAGGTGCGCTTCCGCCAGGACGGTGTGTTGCAGAGCTACAGCGA
ACCGCTGCCCAGTCGCCTGGTGCCAGCCGTGACCTCACGCTTCAAGATCATGGCCGACCTCGATATCGCCGAAAGACGGC
AAGCCCAAGACGGTCGAATCCGCCGCCGCTTTCGCCAACGCAGCGTTGATTTCCGCGTCAGCAGTCTGCCGAGCCGCTTC
GGGGAAAAGATCGTGCTGCGGCTCCTCGACAGCTCATCCACGCAACTGGGCCTCGACAAGCTGATCAGCAACCCGGAGGC
ACTCGAACTGGTGCGCAGCCTCGGCTCCAAGCCATTCGGCATGATCCTGGTGACTGGTCCAACAGGATCAGGAAAATCGA
CGACTCTCTACTCGCTGCTGGCGGAACGCAACGATCCCGGGATCAATATCTCAACGGTGGAGGATCCAATCGAATACACC
CTCCCAGGCATCACCCAATGCCAGGTGAACCGGGAAAAGGGCTTCGACTTCAGCACGGCCCTACGGGCGTTCATGCGACA
AGACCCAGACGTGCTTCTCGTGGGAGAGACCCGTGATCTAGAAACAGCCAAAACAGCGATCGAAGCTGCCCTGACCGGCC
ACCTGGTGCTCACCACCCTGCACTGCAACGACGCCCCAAGCGCTATCGCCCGCCTTGGCGAGATGGGGGTGGAACCCTTC
ATGGTCAGCGCTTCCCTGATCGGGATTGTGTCGCAACGGCTGCTACGGCGTGTCTGTAGCCATTGCCGCGAAAGCTATCA
ACCCGATGCCACTGAACTGGGCCGATTCGGGCTCATGACGCACCATGAGACAGGTGTGCGCTTCTACCGAGCCCACCACC
ACGATGGCAATCGTGACCACAGTGGCGACCCCTGTCCCCACTGTCAGGGCAGCGGTTATCGAGGCCGGATCGGTGTGTAC
GAAGTGCTGCGCATGAACGAGCAGTTGGCCACGGCTGTGGCCAACGGTGCCACCACCGATGTGCTCCGTCGTCTGGCTCT
GGAATCGGGCATGAAAACCCTGCTGGGCTACAGCCTCGAGTTGGTGCGTCAGGGTCAGACCACCTTGGAAGAAGTGGGCC
GGATGATCCTCACCGACTCAGGCCTGGAGTCGGAGCGACGAGCCAGGGCTCTCAGCACCGTCACCTGTGAACGATGTGGT
GGCGGACTACAGGAAGGCTGGCTGGAATGTCCGTACTGTCTGACCCCTCGTCAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(150-417)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

62.309

79.412

0.495