Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB56_RS11685 Genome accession   NZ_CP138955
Coordinates   2121536..2122609 (-) Length   357 a.a.
NCBI ID   WP_413324102.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9503     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2116536..2127609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB56_RS11660 - 2116792..2117400 (+) 609 WP_413324097.1 RNA recognition motif domain-containing protein -
  SIB56_RS11665 dusA 2117427..2118431 (-) 1005 WP_413324098.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB56_RS11670 msrB 2118491..2119009 (+) 519 WP_413324099.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB56_RS11675 - 2118939..2120267 (+) 1329 WP_413324100.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB56_RS11680 - 2120242..2121519 (-) 1278 WP_413324101.1 type II secretion system F family protein -
  SIB56_RS11685 pilT 2121536..2122609 (-) 1074 WP_413324102.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB56_RS11690 pilB/pilB1 2122620..2124350 (-) 1731 WP_413324103.1 GspE/PulE family protein Machinery gene
  SIB56_RS11695 grpE 2124446..2125189 (+) 744 WP_413324104.1 nucleotide exchange factor GrpE -
  SIB56_RS11700 dnaJ 2125232..2126365 (+) 1134 WP_413324105.1 molecular chaperone DnaJ -
  SIB56_RS11705 - 2126368..2126610 (+) 243 WP_413324106.1 sulfurtransferase TusA family protein -
  SIB56_RS11710 rsgA 2126630..2127529 (+) 900 WP_413324107.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39259.83 Da        Isoelectric Point: 6.1087

>NTDB_id=906216 SIB56_RS11685 WP_413324102.1 2121536..2122609(-) (pilT) [Synechococcus sp. MIT S9503]
MELMIEDLMEELVKGGGSDLHIASGQPPYGRFSGQLRAMRDEALLEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSSIPSIELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDRSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRSNPEPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLVSHGEIDLREGLSKASKPTELERLISN

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=906216 SIB56_RS11685 WP_413324102.1 2121536..2122609(-) (pilT) [Synechococcus sp. MIT S9503]
ATGGAGCTGATGATCGAAGACCTGATGGAGGAGCTGGTGAAAGGCGGAGGCAGTGATCTGCATATCGCCAGCGGCCAGCC
CCCCTATGGACGCTTCAGCGGACAGCTGCGGGCGATGCGAGATGAAGCCCTGCTCGAGGAGAGCTGCAACCGGCTGATCT
TCTCAATGCTCAACAACAGCCAACGCAAGACCCTCGAACAGACCTGGGAACTGGACTGCGCCTACGGACTCAAGGGAGTC
GCCAGATTCAGGGTGAATGTCTATCGCCAGAAAGGCAGCTATGCGGCCTGCCTGAGAGCGCTGGGCAGCTCGATTCCCAG
CATCGAGTTGCTCAACCTCCCACCCGTGGTGGTGGAGACCAGCAAGCGACCACGAGGCCTGGTGCTGGTCACCGGTCCCA
CAGGCTCAGGCAAGACCACCACCCTGGCGGCACTGCTGGATCACATCAATCACAGCCGCGCTGAACACATCCTCACCATT
GAGGACCCGATCGAGTTCGTCTACAAGAGCGACCGAAGCCTGGTGCACCAACGCCAGCTCAATGAGGACACCCGCAGCTT
CGGGAATGCACTCAGGGCGGCTCTGCGCGAAGACCCGGACGTGATCCTGGTGGGTGAGATGCGCGACCTGGAAACGATTC
AACTGGCGATCAGCGCCGCCGAAACAGGTCACCTTGTGTTCGGAACGCTGCACACCAGTTCCGCAGCGCAGACGGTCGAC
CGCATGGTTGACGTGTTCCCGCCGGCGCAGCAGACCCAGATCCGCGTGCAGCTGTCAGGAAGTCTGGTGGCGGTGTTCTC
CCAGACGCTGTGTCGCCGCAGCAATCCCGAGCCTGGTCAGTTCGGCCGAGTGATGGCACAGGAAATCATGATCAACACCC
CCGCCATTGCCAACCTGATCCGCGAAGGCAAGACCGCCCAGCTTTACTCACAGATTCAGACCGGTGGTGAACTGGGCATG
CAGACGCTCGAGAAAGCCCTGGCAGATCTCGTGAGTCATGGTGAGATCGATCTGCGGGAAGGACTCTCAAAAGCCAGCAA
ACCCACCGAGCTTGAACGGCTGATCAGCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.198

96.359

0.513
  pilT Pseudomonas aeruginosa PAK

53.198

96.359

0.513
  pilT Acinetobacter nosocomialis M2

51.453

96.359

0.496
  pilT Acinetobacter baumannii D1279779

51.163

96.359

0.493
  pilT Acinetobacter baumannii strain A118

51.163

96.359

0.493
  pilT Acinetobacter baylyi ADP1

51.163

96.359

0.493
  pilT Legionella pneumophila strain ERS1305867

53.012

92.997

0.493
  pilT Legionella pneumophila strain Lp02

53.012

92.997

0.493
  pilT Vibrio cholerae strain A1552

50.882

95.238

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.882

95.238

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.008

98.88

0.485
  pilT Neisseria meningitidis 8013

50.599

93.557

0.473
  pilT Neisseria gonorrhoeae MS11

50.299

93.557

0.471
  pilU Pseudomonas stutzeri DSM 10701

38.177

98.319

0.375
  pilU Vibrio cholerae strain A1552

38.824

95.238

0.37
  pilU Acinetobacter baylyi ADP1

37.069

97.479

0.361