Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB90_RS03650 Genome accession   NZ_CP138946
Coordinates   757055..758131 (-) Length   358 a.a.
NCBI ID   WP_063420781.1    Uniprot ID   -
Organism   Prochlorococcus sp. MIT 1227     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 752055..763131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB90_RS03625 - 752405..752944 (+) 540 WP_413352112.1 RNA recognition motif domain-containing protein -
  SIB90_RS03630 dusA 752954..753958 (-) 1005 WP_063420777.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB90_RS03635 msrB 754028..754534 (+) 507 WP_063420778.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB90_RS03640 - 754461..755783 (+) 1323 WP_063420779.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB90_RS03645 - 755758..757038 (-) 1281 WP_063420780.1 type II secretion system F family protein -
  SIB90_RS03650 pilT 757055..758131 (-) 1077 WP_063420781.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB90_RS03655 pilB/pilB1 758142..760058 (-) 1917 WP_063420782.1 GspE/PulE family protein Machinery gene
  SIB90_RS03660 grpE 760057..760770 (+) 714 WP_063420783.1 nucleotide exchange factor GrpE -
  SIB90_RS03665 dnaJ 760815..761951 (+) 1137 WP_063404335.1 molecular chaperone DnaJ -
  SIB90_RS03670 - 761948..762193 (+) 246 WP_011129402.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39362.78 Da        Isoelectric Point: 5.9277

>NTDB_id=906127 SIB90_RS03650 WP_063420781.1 757055..758131(-) (pilT) [Prochlorococcus sp. MIT 1227]
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELRPMTNTPLSEETCNKLIFSMLNNSQRKTLEQIWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIESLNLPSVVIETSKRPRGLVLVTGPTGSGKTTTLASLLDHINHTRSEHILTI
EDPIEFVYKSDLSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTNSAAQTVD
RIVDVFPPNQQTQVRVQLSGSLLAIFSQTLCRRQNPQPEQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLTKQGEISRSEAIAKASKPSELEQLLNDG

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=906127 SIB90_RS03650 WP_063420781.1 757055..758131(-) (pilT) [Prochlorococcus sp. MIT 1227]
ATGGATCTAATGATCGAGGACCTGATGGAGCAGCTGGTACAAGGTGGCGGCAGTGATCTACACATTGCTACCGGCCAGCC
CCCGTATGGACGATTCAGCGGTGAATTAAGGCCAATGACCAATACCCCCCTATCCGAAGAAACATGCAACAAGCTGATTT
TCTCAATGCTCAACAACAGTCAAAGAAAGACTCTTGAACAAATCTGGGAGCTTGACTGTGCCTATGGGCTAAAGGGTGTG
GCGCGCTTTCGTGTGAATGTCTATCGACAGAAGGGAAGTTACGCCGCCTGTCTACGTGCTCTTGGCAGCACTATCCCGAG
TATTGAAAGTCTCAACCTGCCATCAGTAGTCATAGAAACCAGCAAACGACCAAGGGGATTAGTGCTAGTAACAGGACCAA
CCGGATCAGGCAAAACAACAACCCTTGCTTCCCTGCTGGACCACATCAATCACACCAGAAGCGAACATATCCTCACGATC
GAAGATCCAATCGAATTCGTATACAAAAGCGATCTCAGCCTTGTGCACCAGCGACAGCTCAATGAAGACACACGCAGTTT
TGGCAATGCCCTGCGTGCGGCACTACGCGAAGATCCAGATGTGATCCTCGTGGGCGAAATGCGCGACCTTGAAACCATTC
AACTGGCCATCAGCGCCGCCGAAACCGGTCACCTCGTATTCGGCACCTTGCACACCAACTCGGCGGCACAAACGGTCGAT
CGCATAGTTGATGTTTTCCCCCCAAACCAGCAAACCCAAGTCAGAGTTCAACTCTCAGGAAGTCTGCTCGCCATTTTTTC
GCAAACCCTCTGCCGTCGCCAAAACCCACAACCAGAACAATTTGGGCGGGTCATGGCTCAAGAAATCTTGATCAACACCC
CAGCCACAGCAAACCTGATCAGAGAAGGCAAAACCGCACAGCTCTACTCCCAAATTCAAACCGGAGGAGAACTAGGGATG
CAAACACTAGAAAAAGCCCTTGCGAATCTGACTAAACAGGGTGAAATTAGCCGCTCTGAAGCCATCGCCAAAGCCAGTAA
ACCCAGCGAACTGGAGCAACTCCTCAACGATGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.198

96.089

0.511
  pilT Pseudomonas stutzeri DSM 10701

52.616

96.089

0.506
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.858

98.603

0.492
  pilT Legionella pneumophila strain Lp02

51.929

94.134

0.489
  pilT Legionella pneumophila strain ERS1305867

51.929

94.134

0.489
  pilT Acinetobacter baylyi ADP1

50.872

96.089

0.489
  pilT Neisseria gonorrhoeae MS11

50.287

97.207

0.489
  pilT Neisseria meningitidis 8013

50.729

95.81

0.486
  pilT Acinetobacter nosocomialis M2

49.568

96.927

0.48
  pilT Acinetobacter baumannii strain A118

49.568

96.927

0.48
  pilT Acinetobacter baumannii D1279779

49.568

96.927

0.48
  pilT Vibrio cholerae strain A1552

48.824

94.972

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.824

94.972

0.464
  pilU Pseudomonas stutzeri DSM 10701

40.96

98.883

0.405
  pilU Acinetobacter baylyi ADP1

37.644

97.207

0.366
  pilU Vibrio cholerae strain A1552

38.235

94.972

0.363