Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SIB90_RS03655 Genome accession   NZ_CP138946
Coordinates   758142..760058 (-) Length   638 a.a.
NCBI ID   WP_063420782.1    Uniprot ID   -
Organism   Prochlorococcus sp. MIT 1227     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 753142..765058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB90_RS03635 msrB 754028..754534 (+) 507 WP_063420778.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB90_RS03640 - 754461..755783 (+) 1323 WP_063420779.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB90_RS03645 - 755758..757038 (-) 1281 WP_063420780.1 type II secretion system F family protein -
  SIB90_RS03650 pilT 757055..758131 (-) 1077 WP_063420781.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB90_RS03655 pilB/pilB1 758142..760058 (-) 1917 WP_063420782.1 GspE/PulE family protein Machinery gene
  SIB90_RS03660 grpE 760057..760770 (+) 714 WP_063420783.1 nucleotide exchange factor GrpE -
  SIB90_RS03665 dnaJ 760815..761951 (+) 1137 WP_063404335.1 molecular chaperone DnaJ -
  SIB90_RS03670 - 761948..762193 (+) 246 WP_011129402.1 sulfurtransferase TusA family protein -
  SIB90_RS03675 rsgA 762180..763145 (+) 966 WP_063420784.1 ribosome small subunit-dependent GTPase A -
  SIB90_RS03680 - 763102..763443 (-) 342 WP_063420785.1 YbaB/EbfC family nucleoid-associated protein -
  SIB90_RS03685 murB 763468..764391 (-) 924 WP_063420786.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 638 a.a.        Molecular weight: 70422.39 Da        Isoelectric Point: 6.3645

>NTDB_id=906128 SIB90_RS03655 WP_063420782.1 758142..760058(-) (pilB/pilB1) [Prochlorococcus sp. MIT 1227]
MLTVCKFTSRHVDGSSTPPTSGGSPHLQFHFLFVTQGRPIPKATNSIQQRLELELLLQVSVLSHEELLVGVELMANHTTL
DISTWQQFQAMPINMHNQHLIVAISDQCNEQTKNQLISVLQSKGFSTEFRLALASDISQLLAPMRSEQHIESASTSKATK
PLAQTPTSLLAGFSAEGVLEEDPEEQARIASSVEDLESSLMDSDSSPVINLVDRILLEALQTEASDVHVEPQQDGLQIRF
RQDGVLQRYIEPLPSRLIPAVTSRFKIMADLDIAERRMAQDGRIRRTYRNRMVDFRVNSLPSRYGEKICLRLLDSSAPQL
GLDKLISNPSALSLVRNLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNREKGFD
FSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDASSAIARLNEMGVEPFMVSASLIGIVSQRLLR
RVCRSCRKSYHPTEKELGRFGLMAHTETGVTFFKAHHHGQEKQPCPNCQGSGYKGRVGVYEVLRMNEELATAVAKGATTD
LVRRLALEAGMKTLLGYSLDLVREGHTTLEEVGRMILTDSGLESERRARALSTLTCNVCGAGLQDDWLECPYCLTARQ

Nucleotide


Download         Length: 1917 bp        

>NTDB_id=906128 SIB90_RS03655 WP_063420782.1 758142..760058(-) (pilB/pilB1) [Prochlorococcus sp. MIT 1227]
ATGCTGACCGTTTGCAAATTCACGTCTAGACATGTTGATGGGAGTAGCACACCTCCCACAAGCGGCGGAAGCCCGCACCT
CCAATTTCATTTCTTGTTCGTGACCCAGGGCCGACCAATCCCCAAAGCAACCAACAGCATCCAGCAGCGACTGGAACTGG
AACTGCTCCTACAAGTCAGTGTGCTTAGTCACGAAGAACTTCTTGTCGGAGTGGAGCTAATGGCCAACCACACCACTCTT
GACATCTCAACGTGGCAACAATTCCAGGCCATGCCAATCAACATGCACAACCAACATTTGATTGTAGCCATCTCGGACCA
ATGCAATGAGCAAACCAAAAATCAACTAATCTCAGTGCTGCAGTCCAAAGGCTTCAGCACAGAATTCCGTCTCGCGCTGG
CATCCGACATCAGCCAGCTGCTTGCACCAATGAGATCTGAGCAGCACATTGAATCTGCATCCACGTCAAAAGCAACAAAG
CCACTTGCACAAACACCAACCTCCCTGCTGGCAGGATTTAGTGCCGAAGGCGTGCTCGAAGAAGATCCTGAAGAACAAGC
CAGAATTGCTAGTTCCGTAGAGGATTTGGAGTCCAGCTTGATGGACTCAGACAGCTCACCAGTCATCAACCTGGTGGACC
GCATACTGCTAGAGGCACTGCAAACAGAAGCCAGCGACGTTCACGTTGAGCCGCAACAAGACGGACTACAGATCCGCTTC
CGTCAAGACGGTGTCCTGCAGCGCTATATCGAACCCCTTCCGAGCCGGTTGATCCCTGCTGTTACCTCACGCTTCAAGAT
CATGGCCGACCTAGACATCGCAGAACGGCGCATGGCTCAGGACGGTCGGATTCGTCGCACATATCGCAATCGTATGGTCG
ATTTCAGGGTTAATAGCCTGCCAAGCCGTTACGGGGAAAAAATCTGTCTGCGACTCCTTGACAGCAGTGCTCCACAACTC
GGGCTAGACAAACTGATCAGCAACCCAAGTGCTCTCTCTCTCGTACGCAACTTGGGCTCTAAGCCCTTCGGCATGATCCT
TGTGACAGGTCCAACAGGATCAGGCAAGTCAACAACCCTTTACTCCCTACTGGCAGAACGCAACGAGCCTGGTATCAACA
TTTCCACCGTAGAAGATCCCATCGAATACACCCTCCCTGGGATTACCCAATGTCAAGTGAACAGGGAAAAAGGTTTCGAT
TTCAGTACTGCACTGAGAGCCTTCATGCGTCAAGACCCAGACGTTCTGCTGGTCGGTGAAACCCGTGACCTAGAAACCGC
AAAAACCGCCATCGAAGCCGCACTGACTGGTCATCTGGTGCTAACCACACTGCATTGCAATGACGCATCAAGTGCGATCG
CCCGCCTCAACGAGATGGGCGTGGAACCATTCATGGTTAGCGCCTCGTTGATTGGCATCGTCTCTCAACGACTTCTACGA
AGAGTGTGCCGTTCTTGCCGTAAGTCCTATCACCCAACTGAAAAAGAACTGGGGCGATTCGGTTTGATGGCCCATACAGA
AACTGGCGTGACCTTTTTCAAGGCCCACCATCACGGTCAAGAAAAACAGCCGTGCCCCAACTGTCAAGGCAGCGGCTACA
AGGGCCGAGTTGGTGTCTATGAAGTACTGCGCATGAACGAAGAGCTCGCTACAGCCGTCGCCAAAGGTGCCACTACTGAT
TTAGTAAGACGTCTGGCGCTCGAGGCTGGTATGAAAACTCTGTTGGGCTACAGCCTTGACCTGGTGCGAGAAGGCCACAC
CACCCTTGAGGAAGTAGGCCGCATGATCCTCACCGATTCCGGATTGGAATCAGAGCGCCGCGCCAGAGCCCTTAGCACTC
TCACCTGCAACGTCTGCGGGGCAGGCCTCCAAGACGACTGGCTGGAATGTCCCTACTGTCTAACCGCTCGCCAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-148)

Type II/IV secretion system protein (T2SSE)

(215-481)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

53.795

94.984

0.511