Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB58_RS14530 Genome accession   NZ_CP138942
Coordinates   2664534..2665607 (+) Length   357 a.a.
NCBI ID   WP_067327150.1    Uniprot ID   A0A163XPL9
Organism   Synechococcus sp. MIT S9504     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659534..2670607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB58_RS14505 rsgA 2659635..2660516 (-) 882 WP_066908899.1 ribosome small subunit-dependent GTPase A -
  SIB58_RS14510 - 2660536..2660778 (-) 243 WP_066908608.1 sulfurtransferase TusA family protein -
  SIB58_RS14515 dnaJ 2660781..2661911 (-) 1131 WP_414494673.1 molecular chaperone DnaJ -
  SIB58_RS14520 grpE 2661954..2662691 (-) 738 WP_066908614.1 nucleotide exchange factor GrpE -
  SIB58_RS14525 pilB/pilB1 2662787..2664523 (+) 1737 WP_067327148.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  SIB58_RS14530 pilT 2664534..2665607 (+) 1074 WP_067327150.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB58_RS14535 - 2665624..2666901 (+) 1278 WP_066908623.1 type II secretion system F family protein -
  SIB58_RS14540 - 2666895..2668205 (-) 1311 WP_066908626.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB58_RS14545 msrB 2668135..2668644 (-) 510 WP_066908629.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB58_RS14550 dusA 2668714..2669718 (+) 1005 WP_067327153.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB58_RS14555 - 2669744..2670367 (-) 624 WP_414494674.1 RNA recognition motif domain-containing protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39161.67 Da        Isoelectric Point: 5.4513

>NTDB_id=906094 SIB58_RS14530 WP_067327150.1 2664534..2665607(+) (pilT) [Synechococcus sp. MIT S9504]
MELMIEDLMEELVKGGGSDLHIASGQPPYGRFSGQLRSMRDEALLEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSSIPSIEMLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRAEHILTI
EDPIEFVYKSDQSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRSNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLVTDGDIDQQEALAKASKPSELERLMSS

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=906094 SIB58_RS14530 WP_067327150.1 2664534..2665607(+) (pilT) [Synechococcus sp. MIT S9504]
ATGGAGCTGATGATCGAAGACCTGATGGAAGAGCTGGTGAAAGGCGGGGGCAGTGATCTGCACATCGCCAGCGGCCAGCC
CCCCTACGGACGCTTCAGCGGTCAACTGCGATCGATGCGTGATGAAGCCCTGCTCGAGGAGAGCTGCAACCGGTTGATCT
TCTCAATGCTCAACAACAGCCAGCGCAAGACGCTGGAGCAGACATGGGAACTCGACTGCGCCTACGGCCTCAAGGGAGTG
GCCCGATTCAGGGTGAACGTCTATCGCCAGAAAGGCAGCTATGCGGCCTGTCTGAGAGCGCTCGGAAGCTCGATCCCAAG
CATTGAGATGCTGAACCTGCCACCGGTGGTGGTGGAAACCAGCAAGCGACCAAGGGGTCTGGTGCTGGTCACCGGACCCA
CAGGCTCAGGCAAGACGACCACCCTGGCGGCTCTGCTGGATCACATCAATCACACCCGCGCCGAGCACATCCTCACGATT
GAGGACCCGATCGAGTTCGTCTACAAAAGCGACCAAAGTCTTGTCCACCAGCGCCAGCTCAATGAAGACACCCGCAGCTT
TGGCAATGCGCTCAGGGCAGCACTTCGGGAAGACCCGGACGTGATTCTGGTGGGTGAGATGCGCGACCTGGAGACGATTC
AACTGGCGATCAGCGCCGCAGAGACTGGTCACCTGGTGTTTGGAACTCTGCACACCAGTTCCGCAGCTCAGACGGTGGAC
CGCATGGTGGATGTGTTCCCGCCCGCACAGCAGACCCAGATCCGCGTGCAACTGTCCGGAAGTCTGGTGGCGGTCTTCTC
CCAGACGCTCTGCCGTCGCAGCAATCCCGCGCCTGGCCAGTTCGGCCGGGTGATGGCGCAGGAAATCATGATCAACACTC
CTGCCATCGCCAACCTAATTCGCGAAGGCAAGACGGCCCAGCTCTACTCACAGATCCAGACCGGCGGTGAACTGGGCATG
CAGACCCTGGAGAAAGCGCTGGCAGATCTCGTGACAGATGGCGACATCGATCAACAGGAAGCACTGGCCAAAGCCAGCAA
GCCCTCAGAGCTGGAACGACTTATGAGCAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A163XPL9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.314

97.199

0.518
  pilT Pseudomonas aeruginosa PAK

53.314

97.199

0.518
  pilT Acinetobacter nosocomialis M2

51.297

97.199

0.499
  pilT Acinetobacter baumannii D1279779

51.297

97.199

0.499
  pilT Acinetobacter baumannii strain A118

51.297

97.199

0.499
  pilT Legionella pneumophila strain ERS1305867

52.226

94.398

0.493
  pilT Legionella pneumophila strain Lp02

52.226

94.398

0.493
  pilT Acinetobacter baylyi ADP1

50.872

96.359

0.49
  pilT Vibrio cholerae strain A1552

50.882

95.238

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.882

95.238

0.485
  pilT Neisseria meningitidis 8013

50.146

96.078

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.143

98.039

0.482
  pilT Neisseria gonorrhoeae MS11

49.854

96.078

0.479
  pilU Vibrio cholerae strain A1552

40.118

94.958

0.381
  pilU Pseudomonas stutzeri DSM 10701

38.746

98.319

0.381
  pilU Acinetobacter baylyi ADP1

37.069

97.479

0.361