Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SIB58_RS14525 Genome accession   NZ_CP138942
Coordinates   2662787..2664523 (+) Length   578 a.a.
NCBI ID   WP_067327148.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9504     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2657787..2669523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB58_RS14495 murB 2658362..2659294 (+) 933 WP_066908602.1 UDP-N-acetylmuramate dehydrogenase -
  SIB58_RS14500 - 2659319..2659660 (+) 342 WP_066908605.1 YbaB/EbfC family nucleoid-associated protein -
  SIB58_RS14505 rsgA 2659635..2660516 (-) 882 WP_066908899.1 ribosome small subunit-dependent GTPase A -
  SIB58_RS14510 - 2660536..2660778 (-) 243 WP_066908608.1 sulfurtransferase TusA family protein -
  SIB58_RS14515 dnaJ 2660781..2661911 (-) 1131 WP_414494673.1 molecular chaperone DnaJ -
  SIB58_RS14520 grpE 2661954..2662691 (-) 738 WP_066908614.1 nucleotide exchange factor GrpE -
  SIB58_RS14525 pilB/pilB1 2662787..2664523 (+) 1737 WP_067327148.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  SIB58_RS14530 pilT 2664534..2665607 (+) 1074 WP_067327150.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB58_RS14535 - 2665624..2666901 (+) 1278 WP_066908623.1 type II secretion system F family protein -
  SIB58_RS14540 - 2666895..2668205 (-) 1311 WP_066908626.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB58_RS14545 msrB 2668135..2668644 (-) 510 WP_066908629.1 peptide-methionine (R)-S-oxide reductase MsrB -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63180.76 Da        Isoelectric Point: 4.7937

>NTDB_id=906093 SIB58_RS14525 WP_067327148.1 2662787..2664523(+) (pilB/pilB1) [Synechococcus sp. MIT S9504]
MSDKSRPLELELLLQRTLPGNLGIADEPVLLEELETMGFDPIRHAALAESLTELLSRKPAVSDEAAAPYQEPAITETSQQ
SKAEVATNSQPTAPENSKTETVAETATSYLKDFSVDGVLEADPDEADADSSKPVDLESGLNDAERSPVIDLVDRILMQAL
VLGASDIHVEPQQAGLQLRFRQDGVLQRQIEPLPSRLVPAVTSRFKIMAELDIAERRLPQDGRIRRRFQNRTVDFRVNSL
PSRYGEKIVLRLLDSSATQLGLDKLISDPDALKLVRTLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDP
IEYALPGITQCQVNREKGFDFSQALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLDEMG
VEPFMVSASLIGIVSQRLLRRVCSKCRIAYRPDSEELGRFGLMASNEANVRFFRANHHEGEANPCPQCQGSGYKGRVGVY
EVLRMNEALATTVAKGATTDMVRQLALEGGMKTLLGYSLDLVREGHTTLEEVGRMVLTDSGLESERRARALSTLTCSGCG
GGLQEGWLECPYCLTPRH

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=906093 SIB58_RS14525 WP_067327148.1 2662787..2664523(+) (pilB/pilB1) [Synechococcus sp. MIT S9504]
GTGAGCGACAAAAGCCGGCCTCTGGAACTGGAATTGCTCCTTCAGAGGACCCTTCCAGGCAACCTCGGCATCGCTGATGA
ACCGGTTCTGCTCGAAGAACTGGAGACCATGGGATTTGATCCCATTCGTCATGCCGCGCTTGCGGAGAGCCTGACGGAAC
TGCTCAGCAGGAAACCCGCTGTCAGCGATGAAGCTGCTGCGCCCTACCAGGAACCGGCAATCACGGAAACATCGCAGCAA
TCCAAGGCTGAAGTGGCGACCAACAGCCAGCCCACGGCTCCGGAAAATTCAAAAACCGAAACTGTGGCGGAGACCGCCAC
CTCCTACCTCAAAGATTTCAGCGTTGACGGGGTGCTGGAGGCTGATCCCGATGAGGCGGACGCCGACTCCTCGAAACCGG
TCGATCTGGAATCCGGGCTCAACGATGCAGAACGCTCACCGGTGATCGATCTGGTGGATCGCATCCTGATGCAGGCCCTT
GTACTGGGTGCCAGCGACATCCATGTGGAGCCTCAGCAGGCCGGCCTCCAACTGCGCTTTCGTCAGGACGGCGTGCTGCA
GAGACAGATCGAACCGCTTCCGAGCCGGCTGGTACCGGCCGTGACCTCCAGATTCAAGATCATGGCGGAACTCGACATCG
CTGAACGCCGGCTCCCTCAGGACGGCCGCATCCGACGCCGCTTCCAGAACAGGACCGTGGATTTCCGGGTCAACAGTCTG
CCGAGCCGCTACGGGGAGAAAATCGTTCTGCGGCTTCTGGACAGCTCCGCCACGCAGCTGGGGCTTGACAAGCTGATTTC
CGATCCTGATGCCCTAAAGCTGGTGCGCACCCTGGGGTCCAAACCCTTCGGAATGATCCTGGTCACAGGACCGACCGGAT
CAGGGAAGTCCACAACGCTGTACTCCCTGCTGGCGGAACGCAACGAACCGGGGATCAACATTTCCACGGTGGAGGATCCG
ATCGAATACGCCCTGCCGGGGATCACCCAATGTCAGGTGAACCGAGAAAAAGGCTTCGATTTCAGCCAGGCACTGAGGGC
CTTCATGCGTCAGGACCCGGACGTGCTGCTGGTGGGTGAAACGCGCGATCTGGAAACGGCGAAAACAGCCATCGAAGCGG
CACTCACCGGTCACCTGGTGCTGACCACCCTCCACTGCAATGACGCTCCAAGCGCCATCGCCCGCCTCGACGAGATGGGC
GTGGAGCCGTTCATGGTCAGTGCCTCACTGATCGGGATCGTCTCCCAGCGTCTGCTTCGGCGGGTCTGCTCCAAGTGCCG
AATCGCCTACCGGCCTGATTCCGAGGAACTGGGTCGCTTCGGCCTGATGGCTAGCAACGAGGCAAACGTCAGGTTTTTTA
GAGCCAACCACCATGAAGGCGAGGCCAATCCCTGCCCCCAGTGTCAGGGCAGTGGTTACAAAGGGCGAGTGGGGGTCTAC
GAAGTGCTGCGCATGAATGAGGCGCTGGCAACCACCGTGGCCAAAGGTGCCACCACTGACATGGTGCGCCAGCTGGCCCT
TGAGGGAGGCATGAAGACCCTTCTGGGCTACAGCCTCGATCTGGTGAGGGAGGGACACACGACCCTGGAGGAAGTAGGCC
GCATGGTGCTCACCGATTCCGGCCTCGAATCGGAGCGACGGGCCAGAGCCCTGAGCACGCTCACCTGCAGCGGCTGTGGT
GGCGGACTCCAGGAAGGTTGGCTGGAATGTCCGTACTGCCTGACCCCACGTCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(155-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

55.81

98.27

0.548