Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB64_RS01745 Genome accession   NZ_CP138939
Coordinates   346613..347689 (+) Length   358 a.a.
NCBI ID   WP_413441623.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S1220     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 341613..352689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB64_RS01720 rsgA 341672..342580 (-) 909 WP_413442820.1 ribosome small subunit-dependent GTPase A -
  SIB64_RS01725 - 342564..342803 (-) 240 WP_413441620.1 sulfurtransferase TusA family protein -
  SIB64_RS01730 dnaJ 342803..343936 (-) 1134 WP_413441621.1 molecular chaperone DnaJ -
  SIB64_RS01735 grpE 343936..344604 (-) 669 WP_413441622.1 nucleotide exchange factor GrpE -
  SIB64_RS01740 pilB/pilB1 344842..346602 (+) 1761 WP_413442821.1 GspE/PulE family protein Machinery gene
  SIB64_RS01745 pilT 346613..347689 (+) 1077 WP_413441623.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB64_RS01750 - 347708..348964 (+) 1257 WP_413441624.1 type II secretion system F family protein -
  SIB64_RS01755 - 349246..350547 (-) 1302 WP_413441625.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB64_RS01760 msrB 350477..350980 (-) 504 WP_413441626.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB64_RS01765 dusA 351022..352029 (+) 1008 WP_413441627.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB64_RS01770 - 352036..352665 (-) 630 WP_413441628.1 RNA recognition motif domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39122.47 Da        Isoelectric Point: 5.6542

>NTDB_id=906055 SIB64_RS01745 WP_413441623.1 346613..347689(+) (pilT) [Synechococcus sp. MIT S1220]
MDLMIEDLMEQLVEGGGSDLHIASGQPPYGRFSGELRPMTESALSEENCNKLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSVELLNLPPVVLETSKRPRGMVLVTGPTGSGKTTTLAALLDHINHTRSEHILTI
EDPIEFVYHSDKSLAHQRQLNEDTRSFSNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRQNPKPGEFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGEQGM
QTLEKALADLVNGGAISRGEAIAKASKPAELERLISDD

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=906055 SIB64_RS01745 WP_413441623.1 346613..347689(+) (pilT) [Synechococcus sp. MIT S1220]
ATGGATCTGATGATCGAAGACCTGATGGAACAACTGGTCGAGGGTGGCGGCAGCGACCTTCACATCGCATCCGGCCAACC
GCCATACGGCCGCTTCAGCGGAGAACTGCGTCCGATGACGGAGAGCGCACTCTCGGAGGAGAACTGCAACAAGCTGATCT
TCTCGATGCTGAACAACAGTCAACGAAAAACCCTTGAGCAGACCTGGGAGCTGGACTGCGCCTACGGACTCAAAGGAGTG
GCTCGCTTCCGCGTCAATGTTTACCGCCAGAAGGGCAGCTATGCCGCCTGCCTCCGTGCGCTCGGAAGCACGATTCCAAG
CGTTGAACTGCTCAACCTGCCTCCGGTAGTGCTGGAAACAAGCAAGCGACCACGGGGCATGGTTCTTGTCACCGGCCCAA
CAGGCTCAGGGAAAACCACCACGCTGGCCGCCCTGCTCGATCACATCAACCACACTCGAAGCGAACACATCCTCACGATC
GAAGATCCGATCGAATTCGTTTATCACAGCGACAAAAGTCTGGCGCACCAAAGACAGCTCAATGAAGACACCCGCAGCTT
CTCCAACGCACTGCGCGCCGCACTTCGGGAAGATCCGGACGTGATCCTGGTGGGTGAAATGCGCGACCTGGAGACCATCC
AGCTTGCGATCAGTGCCGCTGAAACAGGGCACCTGGTTTTTGGAACACTCCACACCAGCTCGGCTGCACAAACCGTTGAC
CGGATGGTGGACGTCTTTCCCCCCGCCCAGCAAACACAGATCCGTGTTCAGCTTTCCGGAAGCCTGGTGGCTGTGTTTTC
TCAAACGCTCTGCCGGCGCCAGAACCCGAAACCTGGAGAATTCGGTCGCGTGATGGCCCAAGAGATCCTAATCAACACGC
CCGCCACAGCGAACCTGATTCGAGAAGGCAAGACAGCCCAGCTTTATTCCCAGATTCAGACCGGAGGGGAGCAAGGAATG
CAAACCCTGGAAAAAGCGTTGGCCGATCTGGTGAACGGCGGCGCTATCTCCCGAGGTGAAGCGATTGCCAAAGCGAGCAA
ACCCGCAGAACTTGAACGTCTGATCAGCGATGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.198

96.089

0.511
  pilT Pseudomonas aeruginosa PAK

53.198

96.089

0.511
  pilT Acinetobacter nosocomialis M2

51.163

96.089

0.492
  pilT Acinetobacter baumannii D1279779

51.163

96.089

0.492
  pilT Acinetobacter baumannii strain A118

51.163

96.089

0.492
  pilT Legionella pneumophila strain Lp02

51.929

94.134

0.489
  pilT Legionella pneumophila strain ERS1305867

51.929

94.134

0.489
  pilT Neisseria meningitidis 8013

51.94

93.575

0.486
  pilT Acinetobacter baylyi ADP1

50.581

96.089

0.486
  pilT Neisseria gonorrhoeae MS11

51.642

93.575

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.442

98.603

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.412

94.972

0.469
  pilT Vibrio cholerae strain A1552

49.412

94.972

0.469
  pilU Pseudomonas stutzeri DSM 10701

38.177

98.045

0.374
  pilU Vibrio cholerae strain A1552

38.824

94.972

0.369
  pilU Acinetobacter baylyi ADP1

37.356

97.207

0.363