Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SIB64_RS01740 Genome accession   NZ_CP138939
Coordinates   344842..346602 (+) Length   586 a.a.
NCBI ID   WP_413442821.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S1220     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339842..351602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB64_RS01710 murB 340438..341331 (+) 894 WP_413441618.1 UDP-N-acetylmuramate dehydrogenase -
  SIB64_RS01715 - 341356..341697 (+) 342 WP_413441619.1 YbaB/EbfC family nucleoid-associated protein -
  SIB64_RS01720 rsgA 341672..342580 (-) 909 WP_413442820.1 ribosome small subunit-dependent GTPase A -
  SIB64_RS01725 - 342564..342803 (-) 240 WP_413441620.1 sulfurtransferase TusA family protein -
  SIB64_RS01730 dnaJ 342803..343936 (-) 1134 WP_413441621.1 molecular chaperone DnaJ -
  SIB64_RS01735 grpE 343936..344604 (-) 669 WP_413441622.1 nucleotide exchange factor GrpE -
  SIB64_RS01740 pilB/pilB1 344842..346602 (+) 1761 WP_413442821.1 GspE/PulE family protein Machinery gene
  SIB64_RS01745 pilT 346613..347689 (+) 1077 WP_413441623.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB64_RS01750 - 347708..348964 (+) 1257 WP_413441624.1 type II secretion system F family protein -
  SIB64_RS01755 - 349246..350547 (-) 1302 WP_413441625.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB64_RS01760 msrB 350477..350980 (-) 504 WP_413441626.1 peptide-methionine (R)-S-oxide reductase MsrB -

Sequence

Protein


Download         Length: 586 a.a.        Molecular weight: 64530.16 Da        Isoelectric Point: 4.9728

>NTDB_id=906054 SIB64_RS01740 WP_413442821.1 344842..346602(+) (pilB/pilB1) [Synechococcus sp. MIT S1220]
MDRAAWSAFPAMPLALDAHHLDLAVPSHWNEADRLNLISRLSGIKRELRLHPTLESDLNAVLALPEQQKKPQEQQLQNPA
AKSLPALSAEPQANSEPEQTQQKIESGASVEETASSFLEGFSADGILDDEIDDDAQLNSNTIDLEESLRDAEASPVVTLV
DRILLQAMSVSASDIHVEPQQKGLRLRYRQDGVLQQYVEPLPNRLIPAVTSRFKILADLDIAERRQAQDGRIRRKYKNRV
IDFRVNTLPSRFGEKICLRLLDSSATQLGLDKLVSDPNALDLIRELGSKPFGMILVTGPTGSGKSTTLYSLLSERNDPGL
NISTVEDPIEYTLPGITQCQVNREKGFDFATALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLSTLHANDAPST
IARLDEMGVEPFMVAAALIGIVSQRLLRRVCSTCRESFQPSEQELGRFGLMASREANVTFYRAHQHKDGEPICPTCNGSG
YKGRVGAFEVLRINEEISTAISKGSTTDVLRQLALESGMVTLLGYSLELVRRGHTTLEEVGRMILTDSGLESERRARALS
TMTCEGCGAGLQEGWLECPYCLTPRH

Nucleotide


Download         Length: 1761 bp        

>NTDB_id=906054 SIB64_RS01740 WP_413442821.1 344842..346602(+) (pilB/pilB1) [Synechococcus sp. MIT S1220]
ATGGATCGCGCGGCATGGTCGGCATTTCCAGCCATGCCGCTCGCACTCGATGCGCACCATCTCGATCTAGCTGTTCCAAG
CCATTGGAATGAAGCAGATCGGTTAAACCTGATCAGTCGCTTATCCGGTATCAAGCGGGAGCTCCGCCTTCATCCCACCC
TGGAAAGCGATCTGAACGCGGTTCTGGCCCTGCCGGAACAGCAAAAAAAGCCACAAGAGCAACAGTTACAGAACCCCGCT
GCAAAATCATTGCCGGCGTTATCAGCCGAGCCACAAGCCAACTCCGAACCAGAGCAGACCCAACAGAAGATCGAGAGCGG
AGCCTCAGTCGAGGAGACCGCATCCAGTTTTCTGGAGGGATTCAGTGCCGATGGGATTCTTGACGATGAAATCGATGACG
ATGCCCAGCTAAACAGCAACACCATCGATCTTGAGGAGAGCTTGCGGGATGCCGAGGCATCACCCGTGGTGACCCTGGTG
GATCGGATCCTGTTGCAGGCCATGTCCGTGAGTGCCAGCGACATTCACGTTGAGCCACAGCAGAAAGGGCTACGCCTGCG
ATACCGCCAGGACGGCGTACTTCAGCAGTACGTGGAGCCGCTGCCCAATCGCCTGATCCCAGCGGTGACATCCCGTTTCA
AGATCCTGGCGGATCTGGACATCGCTGAACGTCGCCAGGCTCAGGATGGCCGGATCCGGCGTAAATACAAGAACAGGGTC
ATCGATTTTAGGGTCAACACCCTGCCAAGTCGATTCGGCGAAAAAATTTGTCTACGTCTTCTGGACAGCAGCGCCACCCA
GCTGGGGCTGGACAAACTCGTGAGTGACCCCAACGCCCTTGATCTGATCCGAGAGCTCGGATCCAAACCCTTCGGGATGA
TCCTGGTGACTGGTCCCACCGGATCGGGCAAATCGACAACGCTTTATTCGTTGCTATCGGAACGCAACGACCCTGGGCTC
AATATTTCAACCGTTGAAGACCCGATCGAATACACCCTGCCGGGAATCACCCAGTGCCAAGTGAACCGGGAGAAGGGATT
CGATTTCGCAACAGCCCTGCGAGCTTTCATGCGCCAGGATCCGGATGTGCTGCTCGTGGGTGAAACCCGCGATCTTGAAA
CAGCCAAGACCGCGATCGAAGCGGCACTCACTGGGCACCTGGTACTCAGCACCCTTCACGCCAACGACGCACCGAGCACC
ATTGCGCGCCTCGATGAAATGGGCGTCGAGCCGTTCATGGTGGCTGCCGCATTGATCGGGATCGTTTCCCAGCGGCTGCT
GCGACGGGTCTGCAGCACTTGCCGAGAAAGTTTTCAACCGAGTGAACAGGAGCTGGGACGGTTCGGGTTGATGGCCAGCC
GGGAGGCGAACGTGACGTTCTACAGGGCCCATCAACACAAGGATGGTGAACCGATTTGCCCGACCTGCAACGGCAGTGGA
TACAAAGGACGGGTGGGTGCTTTCGAAGTGCTGCGCATCAATGAGGAGATCTCGACTGCAATTTCGAAAGGATCAACCAC
CGATGTTCTCCGGCAGCTCGCCCTGGAATCCGGAATGGTGACGTTGCTCGGCTACAGCCTGGAACTGGTACGGCGAGGCC
ACACCACCCTTGAGGAGGTTGGCCGAATGATTTTGACGGATTCAGGCCTCGAATCCGAGCGCAGGGCGCGTGCTCTAAGC
ACAATGACTTGTGAAGGGTGCGGGGCCGGCCTACAGGAAGGCTGGCTGGAGTGCCCCTACTGCCTGACACCACGACATTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(163-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

53.147

97.611

0.519