Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIC18_RS03010 Genome accession   NZ_CP138915
Coordinates   587912..588985 (-) Length   357 a.a.
NCBI ID   WP_413430498.1    Uniprot ID   -
Organism   Synechococcus sp. Cu2B8-bc1011     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 582912..593985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIC18_RS02985 - 583164..583715 (+) 552 WP_413430120.1 RNA recognition motif domain-containing protein -
  SIC18_RS02990 dusA 583734..584738 (-) 1005 WP_413430121.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIC18_RS02995 msrB 584805..585317 (+) 513 WP_413430122.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIC18_RS03000 - 585352..586557 (+) 1206 WP_413430497.1 NAD(P)/FAD-dependent oxidoreductase -
  SIC18_RS03005 - 586627..587895 (-) 1269 WP_413430123.1 type II secretion system F family protein -
  SIC18_RS03010 pilT 587912..588985 (-) 1074 WP_413430498.1 type IV pilus twitching motility protein PilT Machinery gene
  SIC18_RS03015 pilB/pilB1 588997..590769 (-) 1773 WP_371190312.1 GspE/PulE family protein Machinery gene
  SIC18_RS03020 grpE 590849..591577 (+) 729 WP_048347610.1 nucleotide exchange factor GrpE -
  SIC18_RS03025 dnaJ 591620..592750 (+) 1131 WP_048347611.1 molecular chaperone DnaJ -
  SIC18_RS03030 - 592754..592993 (+) 240 WP_048347612.1 sulfurtransferase TusA family protein -
  SIC18_RS03035 rsgA 593013..593894 (+) 882 WP_048348405.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39234.71 Da        Isoelectric Point: 5.2541

>NTDB_id=905833 SIC18_RS03010 WP_413430498.1 587912..588985(-) (pilT) [Synechococcus sp. Cu2B8-bc1011]
MELMIEDLMEQLVNGGGSDLHIASGQPPYGRFSGELRPMQEEPLSEEGCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSKIPSIQLLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRAEHILTI
EDPIEFVYTSDLSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
WMVDVFPPGQQTQIRVQLSGSLTAVFSQTLCRRQNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLERALANLIDQGEISLDEGQSKASKPAELERLMNT

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=905833 SIC18_RS03010 WP_413430498.1 587912..588985(-) (pilT) [Synechococcus sp. Cu2B8-bc1011]
ATGGAACTAATGATCGAAGACTTGATGGAACAGCTGGTGAACGGAGGCGGAAGCGACCTCCACATCGCCAGCGGACAGCC
GCCTTACGGCCGCTTCAGTGGCGAACTGCGGCCGATGCAGGAAGAACCGCTCAGTGAAGAAGGCTGCAACCGCCTGATCT
TTTCCATGCTTAATAACAGCCAACGCAAAACACTCGAACAAACGTGGGAGCTGGATTGTGCCTATGGCCTAAAAGGAGTC
GCGCGTTTTCGCGTGAATGTGTACCGGCAGAAAGGCAGCTATGCCGCCTGCTTACGTGCTCTTGGAAGCAAAATCCCCAG
CATTCAGTTGTTGAATCTGCCTCCCGTCGTGGTGGAAACCAGCAAACGCCCCCGGGGTCTTGTCCTGGTCACGGGTCCGA
CTGGCTCCGGCAAAACGACCACGCTCGCAGCGCTTCTCGATCACATCAACCACACGCGGGCTGAGCACATCCTCACCATC
GAAGACCCGATTGAATTCGTCTACACCAGTGATCTCAGCTTGGTGCATCAACGCCAACTCAACGAAGACACGCGCAGTTT
TGCCAACGCTCTGCGCGCTGCTCTACGAGAAGACCCAGACGTGATTTTGGTGGGTGAAATGCGTGATCTTGAAACGATTC
AACTGGCGATCAGTGCTGCAGAGACAGGGCATTTGGTGTTTGGAACATTGCACACCAGTTCTGCAGCCCAGACGGTGGAT
TGGATGGTGGATGTGTTTCCACCTGGCCAGCAAACCCAAATCCGCGTGCAACTCTCAGGAAGCCTGACTGCGGTGTTCTC
TCAAACGCTCTGCCGACGCCAAAATCCTGCCCCTGGGCAATTCGGTCGCGTGATGGCACAAGAAATCATGATCAACACGC
CAGCCATCGCCAATTTGATCCGCGAAGGCAAAACAGCTCAGCTGTATTCCCAAATTCAAACCGGCGGCGAGCTCGGCATG
CAAACCCTGGAAAGGGCCCTTGCCAATCTGATTGATCAGGGTGAAATCAGCCTTGATGAAGGACAAAGCAAAGCCAGCAA
ACCCGCGGAACTGGAACGCCTGATGAACACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-249)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.198

96.359

0.513
  pilT Pseudomonas aeruginosa PAK

53.198

96.359

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.716

98.599

0.49
  pilT Acinetobacter nosocomialis M2

50.581

96.359

0.487
  pilT Acinetobacter baumannii D1279779

50.581

96.359

0.487
  pilT Acinetobacter baumannii strain A118

50.581

96.359

0.487
  pilT Legionella pneumophila strain Lp02

51.952

93.277

0.485
  pilT Legionella pneumophila strain ERS1305867

51.952

93.277

0.485
  pilT Neisseria meningitidis 8013

50.146

96.078

0.482
  pilT Acinetobacter baylyi ADP1

50

96.359

0.482
  pilT Vibrio cholerae strain A1552

50.294

95.238

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

95.238

0.479
  pilT Neisseria gonorrhoeae MS11

49.854

96.078

0.479
  pilU Pseudomonas stutzeri DSM 10701

39.316

98.319

0.387
  pilU Vibrio cholerae strain A1552

37.249

97.759

0.364
  pilU Acinetobacter baylyi ADP1

37.069

97.479

0.361