Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SIC18_RS03015 Genome accession   NZ_CP138915
Coordinates   588997..590769 (-) Length   590 a.a.
NCBI ID   WP_371190312.1    Uniprot ID   -
Organism   Synechococcus sp. Cu2B8-bc1011     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 583997..595769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIC18_RS02995 msrB 584805..585317 (+) 513 WP_413430122.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIC18_RS03000 - 585352..586557 (+) 1206 WP_413430497.1 NAD(P)/FAD-dependent oxidoreductase -
  SIC18_RS03005 - 586627..587895 (-) 1269 WP_413430123.1 type II secretion system F family protein -
  SIC18_RS03010 pilT 587912..588985 (-) 1074 WP_413430498.1 type IV pilus twitching motility protein PilT Machinery gene
  SIC18_RS03015 pilB/pilB1 588997..590769 (-) 1773 WP_371190312.1 GspE/PulE family protein Machinery gene
  SIC18_RS03020 grpE 590849..591577 (+) 729 WP_048347610.1 nucleotide exchange factor GrpE -
  SIC18_RS03025 dnaJ 591620..592750 (+) 1131 WP_048347611.1 molecular chaperone DnaJ -
  SIC18_RS03030 - 592754..592993 (+) 240 WP_048347612.1 sulfurtransferase TusA family protein -
  SIC18_RS03035 rsgA 593013..593894 (+) 882 WP_048348405.1 ribosome small subunit-dependent GTPase A -
  SIC18_RS03040 - 593869..594210 (-) 342 WP_048347613.1 YbaB/EbfC family nucleoid-associated protein -
  SIC18_RS03045 murB 594235..595173 (-) 939 WP_413430124.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 590 a.a.        Molecular weight: 64148.12 Da        Isoelectric Point: 5.3134

>NTDB_id=905834 SIC18_RS03015 WP_371190312.1 588997..590769(-) (pilB/pilB1) [Synechococcus sp. Cu2B8-bc1011]
MSADSTRMPLPPALPAKLISEAELAAGEALLHSGQVLDLETWKQLQALPIHLSDDGLMVAIASSSDRNSREQLKQVLKSH
GYISKLVLANGADLKRILDPEEFNSGSNISQTSISETAKSLLDGFDTEGILSADPDEAEIQNNSASSLDINSSITGASPV
VTLVDRILIKALDMNASDIHVEPQQSGLQIRLRKDGVLTNLAQPLPTKLIPAITSRFKIMADLDIAERRQAQDGRIRRQY
KGRTVDFRVNSLPSRYGEKICLRLLDSQSTQLGLEKLISNPATLELVRTLGSKPFGMILVTGPTGSGKSTTLYSLLAERN
EPGINISTVEDPIEYTLPGITQCQVNREKGFDFSTALRAFMRQDPDVLLVGETRDQETAKTAIEAALTGHLVLTTLHCND
APSAIARLDEMGVEPFMVSASLLGIVSQRLLRRVCSDCRIPYHPDSRELGRFGLMTSDEGNVTFFKAKHHEGPETPCPTC
QGTGYKGRIGVYEVLRMNEALAASVAKGATTDLVRQLALEAGMKTLLGYSLDLVREGHTTLEEVDRMVLTDAGLESEQRA
RALSTVTCRGCGGGLQEGWLECPYCLTPRQ

Nucleotide


Download         Length: 1773 bp        

>NTDB_id=905834 SIC18_RS03015 WP_371190312.1 588997..590769(-) (pilB/pilB1) [Synechococcus sp. Cu2B8-bc1011]
ATGAGCGCCGACTCCACCCGAATGCCGCTACCGCCTGCTCTGCCGGCAAAGCTGATTTCAGAGGCAGAGCTTGCCGCTGG
AGAAGCACTCCTTCATTCCGGGCAGGTTTTAGACCTGGAAACCTGGAAGCAACTTCAGGCGCTTCCCATCCACCTCAGCG
ACGACGGCCTCATGGTGGCCATCGCGAGCAGCAGCGACCGAAACAGTCGTGAACAACTCAAACAGGTTTTAAAAAGCCAT
GGCTACATCTCCAAACTTGTACTGGCCAATGGAGCAGATCTAAAACGGATTCTTGATCCAGAAGAATTCAACTCAGGTTC
AAATATCAGCCAAACATCAATTAGTGAGACAGCAAAATCACTTCTCGATGGATTTGATACTGAAGGAATCTTAAGTGCCG
ATCCAGACGAAGCTGAAATTCAAAACAATTCGGCCTCCTCGCTAGACATCAATTCATCAATAACCGGTGCATCTCCAGTT
GTCACACTGGTGGATCGTATCTTAATTAAGGCATTAGACATGAATGCCAGTGATATCCATGTAGAGCCGCAACAATCAGG
ATTGCAAATTAGGCTTAGAAAGGATGGTGTACTCACCAATTTAGCGCAACCACTCCCCACTAAATTAATCCCAGCGATTA
CCTCTCGCTTCAAGATCATGGCTGATCTTGATATCGCTGAACGGAGGCAAGCACAAGATGGTCGCATCCGTAGACAGTAC
AAAGGACGAACTGTTGACTTTCGCGTCAACAGCCTTCCAAGCCGCTATGGAGAAAAGATTTGCTTGCGGTTACTCGATAG
TCAATCAACTCAGTTAGGGCTAGAAAAACTCATCTCCAATCCCGCCACACTGGAGCTTGTACGAACCCTTGGTTCAAAAC
CCTTCGGCATGATTCTTGTGACCGGACCAACAGGATCTGGTAAATCCACAACTCTGTATTCCTTACTAGCCGAACGCAAC
GAACCTGGGATCAATATCTCCACCGTGGAAGATCCAATTGAATACACATTGCCTGGCATTACTCAATGTCAAGTGAATCG
AGAAAAAGGATTTGACTTCAGCACTGCTCTTCGTGCCTTCATGCGCCAGGACCCAGATGTGCTGTTAGTGGGTGAGACCC
GTGATCAGGAAACAGCAAAAACTGCCATTGAAGCCGCGTTAACAGGGCACCTTGTCTTAACCACCCTTCACTGCAACGAT
GCCCCGAGCGCCATCGCCCGACTCGACGAAATGGGCGTTGAACCCTTCATGGTGAGCGCATCCTTGCTCGGCATCGTTTC
TCAACGCTTACTCAGACGCGTATGCAGCGATTGCCGAATCCCCTACCACCCAGATTCGCGAGAATTAGGCCGCTTTGGCC
TCATGACGAGCGATGAAGGCAATGTCACCTTTTTCAAAGCCAAACACCACGAAGGGCCAGAAACGCCATGCCCCACCTGT
CAGGGCACCGGTTACAAGGGCCGTATCGGTGTGTACGAAGTGTTACGCATGAATGAGGCCCTGGCGGCATCGGTCGCCAA
AGGGGCAACCACTGATCTCGTCCGCCAGTTAGCACTTGAAGCAGGAATGAAAACCTTGCTCGGCTACAGCCTTGATCTCG
TGCGAGAAGGTCACACCACGCTGGAAGAGGTGGACCGCATGGTGTTGACAGATGCGGGTCTGGAATCGGAACAACGTGCC
AGGGCCCTCAGCACAGTCACTTGCCGTGGATGTGGTGGCGGACTGCAAGAAGGTTGGCTGGAATGCCCCTATTGCCTGAC
ACCCCGTCAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(167-433)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

54.545

96.949

0.529