Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Cthiooxydans_RS23430 Genome accession   NZ_AP025193
Coordinates   5095001..5096044 (+) Length   347 a.a.
NCBI ID   WP_003068464.1    Uniprot ID   A0A5S4T2J0
Organism   Comamonas thiooxydans strain NR4028     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5090001..5101044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cthiooxydans_RS23410 (Cthiooxydans_45660) - 5090250..5091848 (-) 1599 WP_087864975.1 AMP-binding protein -
  Cthiooxydans_RS23415 (Cthiooxydans_45670) - 5092013..5092783 (+) 771 WP_087864974.1 TetR/AcrR family transcriptional regulator -
  Cthiooxydans_RS23420 (Cthiooxydans_45680) - 5092830..5094077 (+) 1248 WP_087864973.1 branched-chain amino acid ABC transporter substrate-binding protein -
  Cthiooxydans_RS23425 (Cthiooxydans_45690) - 5094232..5094963 (-) 732 WP_087864972.1 YggS family pyridoxal phosphate-dependent enzyme -
  Cthiooxydans_RS23430 (Cthiooxydans_45700) pilT 5095001..5096044 (+) 1044 WP_003068464.1 type IV pilus twitching motility protein PilT Machinery gene
  Cthiooxydans_RS23435 (Cthiooxydans_45710) pilU 5096190..5097326 (+) 1137 WP_003068462.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Cthiooxydans_RS23440 (Cthiooxydans_45720) - 5097471..5098382 (+) 912 WP_087864971.1 NAD(P)-dependent oxidoreductase -
  Cthiooxydans_RS23445 (Cthiooxydans_45730) - 5098444..5099097 (-) 654 WP_087864970.1 BON domain-containing protein -
  Cthiooxydans_RS23450 (Cthiooxydans_45740) - 5099094..5099702 (-) 609 WP_003068456.1 SIS domain-containing protein -
  Cthiooxydans_RS23455 (Cthiooxydans_45750) - 5099792..5100193 (-) 402 WP_003068453.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38354.25 Da        Isoelectric Point: 6.7743

>NTDB_id=90409 Cthiooxydans_RS23430 WP_003068464.1 5095001..5096044(+) (pilT) [Comamonas thiooxydans strain NR4028]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKTVHAMVYDIMSDAQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSQNMGMQTLDQN
LTDLVRRNLISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=90409 Cthiooxydans_RS23430 WP_003068464.1 5095001..5096044(+) (pilT) [Comamonas thiooxydans strain NR4028]
GTGGATATCACGCAATTGCTGGCATTCAGTGTCAAGAACAAGGCTTCGGACTTGCATTTGTCGGCAGGCCTGCCACCCAT
GATTCGCGTCCACGGCGATGTGCGCCGCATCAACATCGATCCGCTGGACCACAAGACCGTGCACGCCATGGTCTACGACA
TCATGAGCGATGCCCAGCGCAAGCAGTACGAAGAGTTTCTGGAAGTGGACTTTTCGTTCGAGATTGAAGGCCTGGCCCGG
TTTCGTGTCAATGCCTTCAACCAGAACCGCGGCGCGGCGGCCGTGTTCCGTACCATTCCCAGCAAGATCCTGACACTGGA
GCAGCTCAACGCCCCCAAGATCTTCGCCGACCTGGCCCTGAAGCCACGTGGTCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGCCATGGTCAACTATCTCAACGAGACCGAGTACGGCCACATCCTGACGGTGGAAGAT
CCCATCGAATTCGTGCACGAGTCCAAGAAATGCCTGATCAACCAGCGCGAAGTCGGCCCCATGACGCTGTCGTTCGCGGC
AGCGCTGAAGTCCGCGCTGCGTGAAGACCCGGACGCGATTCTGGTCGGCGAAATGCGGGACCTGGAAACCATCCGTCTGG
CCATGACGGCGGCCGAAACCGGGCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCGGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAGTCGTTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGTGTGGCCGCACACGAGATCATGCTGGGCACGCCCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCACAGATGTACTCCACCATTCAGACCAGCCAGAACATGGGCATGCAGACTCTGGACCAGAAC
CTGACCGATCTGGTGCGCCGCAACCTCATCAGTCCGGCCGAAGCGCGCAGCAAGGCGAAGATTCCCGAGAATTTTCCTGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4T2J0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

44.478

96.542

0.429
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409

Multiple sequence alignment