Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Cthiooxydans_RS23435 Genome accession   NZ_AP025193
Coordinates   5096190..5097326 (+) Length   378 a.a.
NCBI ID   WP_003068462.1    Uniprot ID   A0A5S4T690
Organism   Comamonas thiooxydans strain NR4028     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5091190..5102326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cthiooxydans_RS23415 (Cthiooxydans_45670) - 5092013..5092783 (+) 771 WP_087864974.1 TetR/AcrR family transcriptional regulator -
  Cthiooxydans_RS23420 (Cthiooxydans_45680) - 5092830..5094077 (+) 1248 WP_087864973.1 branched-chain amino acid ABC transporter substrate-binding protein -
  Cthiooxydans_RS23425 (Cthiooxydans_45690) - 5094232..5094963 (-) 732 WP_087864972.1 YggS family pyridoxal phosphate-dependent enzyme -
  Cthiooxydans_RS23430 (Cthiooxydans_45700) pilT 5095001..5096044 (+) 1044 WP_003068464.1 type IV pilus twitching motility protein PilT Machinery gene
  Cthiooxydans_RS23435 (Cthiooxydans_45710) pilU 5096190..5097326 (+) 1137 WP_003068462.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Cthiooxydans_RS23440 (Cthiooxydans_45720) - 5097471..5098382 (+) 912 WP_087864971.1 NAD(P)-dependent oxidoreductase -
  Cthiooxydans_RS23445 (Cthiooxydans_45730) - 5098444..5099097 (-) 654 WP_087864970.1 BON domain-containing protein -
  Cthiooxydans_RS23450 (Cthiooxydans_45740) - 5099094..5099702 (-) 609 WP_003068456.1 SIS domain-containing protein -
  Cthiooxydans_RS23455 (Cthiooxydans_45750) - 5099792..5100193 (-) 402 WP_003068453.1 YraN family protein -
  Cthiooxydans_RS23460 (Cthiooxydans_45760) rsmI 5100193..5101116 (+) 924 WP_087864969.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42062.55 Da        Isoelectric Point: 6.7687

>NTDB_id=90410 Cthiooxydans_RS23435 WP_003068462.1 5096190..5097326(+) (pilU) [Comamonas thiooxydans strain NR4028]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGKVTKVSPQPLNQLHTLALARSIMNDRQVADFEHTKECNFAI
SPAGVGRFRVNAFMQQGKVGMVLRTIPSTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTESWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDVSLNLRAIISQRLIPKQDSKGRVAAIEVMLNSPLIADLIFKGEVVEIKEIMKKSRNIG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLNSQRARTLDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=90410 Cthiooxydans_RS23435 WP_003068462.1 5096190..5097326(+) (pilU) [Comamonas thiooxydans strain NR4028]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACTGCAGAGTTTCCGCCCGCCATCAAGATTGACGGCAAGGTCACCAAGGTCTCGCCCCAGCCGCTGAACCAGTTGCATA
CGCTGGCCCTGGCGCGTTCCATCATGAACGACAGGCAGGTGGCGGACTTCGAACACACCAAGGAGTGCAACTTCGCCATC
TCGCCTGCCGGTGTGGGGCGCTTCCGTGTCAACGCCTTCATGCAGCAGGGCAAGGTCGGCATGGTGCTGCGTACCATTCC
CAGCACCCTGCCCACGATTGACGGCCTGGGCGTGCCGCAGGTTCTCAAGGAAGTGACCATGACCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCGACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTGGACTGGCGCAATGAAAACTCCTTCGGC
CACATCATCACCGTGGAAGATCCCGTGGAATTCGTGCATCCGCACAAGAACTGCGTGGTCACGCAACGCGAGGTGGGCCT
GGACACTGAAAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGATGTGATTCTGATGGGCGAAATCCGTG
ACCGAGAAACCATGGAGCACGCGATTGCGTTCTCTGAAACCGGGCATCTATGTCTGGCCACGCTGCACGCCAACAGTGCC
AACCAGGCTCTGGACCGTATCGTCAACTTCTTCCCCGAAGAGCGCCGTCCGCAACTGCTGATGGACGTGTCGCTGAATCT
GCGGGCCATCATTTCCCAGCGCCTGATCCCCAAGCAGGACAGCAAGGGCCGTGTCGCCGCCATCGAGGTGATGCTCAACT
CTCCGCTGATCGCCGACCTGATCTTCAAGGGCGAAGTCGTCGAGATTAAGGAGATCATGAAGAAAAGCCGCAATATCGGC
ATGCAGACCTTTGACCAGGCGCTGTTCGACCTGTTCGAGTCCAATCTCATCACCTATGAAGACGCGCTGCGCAATGCAGA
CTCGGTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCCAGCGTGCCCGGACTCTGGACCTGGCGGCCGGCACCGAGC
ATCTGACCATCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4T690

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.656

96.825

0.587
  pilU Acinetobacter baylyi ADP1

55.71

94.974

0.529
  pilU Vibrio cholerae strain A1552

53.736

92.063

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Acinetobacter baylyi ADP1

42.073

86.772

0.365

Multiple sequence alignment