Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RZE79_RS27665 Genome accession   NZ_CP138496
Coordinates   5894430..5895473 (+) Length   347 a.a.
NCBI ID   WP_056576373.1    Uniprot ID   A0A9X8CZE2
Organism   Variovorax sp. EBFNA2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5889430..5900473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE79_RS27640 (RZE79_27640) - 5890266..5890952 (-) 687 WP_319357693.1 DUF2242 domain-containing protein -
  RZE79_RS27645 (RZE79_27645) - 5891066..5892340 (-) 1275 WP_319357694.1 Glu/Leu/Phe/Val dehydrogenase -
  RZE79_RS27650 (RZE79_27650) - 5892592..5893047 (+) 456 WP_319357695.1 hypothetical protein -
  RZE79_RS27655 (RZE79_27655) - 5893193..5893660 (+) 468 WP_062478450.1 Lrp/AsnC ligand binding domain-containing protein -
  RZE79_RS27660 (RZE79_27660) - 5893677..5894378 (-) 702 WP_319357696.1 YggS family pyridoxal phosphate-dependent enzyme -
  RZE79_RS27665 (RZE79_27665) pilT 5894430..5895473 (+) 1044 WP_056576373.1 type IV pilus twitching motility protein PilT Machinery gene
  RZE79_RS27670 (RZE79_27670) pilU 5895503..5896639 (+) 1137 WP_101493812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RZE79_RS27675 (RZE79_27675) - 5896705..5897613 (+) 909 WP_319357697.1 NAD(P)-dependent oxidoreductase -
  RZE79_RS27680 (RZE79_27680) - 5897759..5898553 (-) 795 WP_319357698.1 BON domain-containing protein -
  RZE79_RS27685 (RZE79_27685) - 5898550..5899200 (-) 651 WP_319357699.1 SIS domain-containing protein -
  RZE79_RS27690 (RZE79_27690) - 5899286..5899657 (-) 372 WP_319357700.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38148.83 Da        Isoelectric Point: 6.8356

>NTDB_id=903164 RZE79_RS27665 WP_056576373.1 5894430..5895473(+) (pilT) [Variovorax sp. EBFNA2]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=903164 RZE79_RS27665 WP_056576373.1 5894430..5895473(+) (pilT) [Variovorax sp. EBFNA2]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGAACGGCGACGTGCGCCGCATCAACGTCGATGCGCTGGACCACAAGGCGGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCGCGCGGCGCGGCCGCCGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTCGA
GCAGCTCAACGCCCCGAAGATTTTCGGCGAACTCGCACTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTGAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGATGACGCTGTCGTTCTCGAA
CGCGCTGCGCTCCGCGTTGCGCGAAGACCCGGACGCCATTCTGGTGGGCGAGTTGCGCGACCTCGAAACCATTCGTCTCG
CGATGACCGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAAAAAGAAATGATCCGCGCGATGCTGTCGGAATCGCTGCAAGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTCGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTCGACCAGAAC
CTGACAGAGCTGGTGCGCCGCAATACGATCTCTGCCGCCGAAGCGCGCGGTAAAGCCAAGATTCCCGAGAATTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398