Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RZE79_RS27670 Genome accession   NZ_CP138496
Coordinates   5895503..5896639 (+) Length   378 a.a.
NCBI ID   WP_101493812.1    Uniprot ID   -
Organism   Variovorax sp. EBFNA2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5890503..5901639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE79_RS27645 (RZE79_27645) - 5891066..5892340 (-) 1275 WP_319357694.1 Glu/Leu/Phe/Val dehydrogenase -
  RZE79_RS27650 (RZE79_27650) - 5892592..5893047 (+) 456 WP_319357695.1 hypothetical protein -
  RZE79_RS27655 (RZE79_27655) - 5893193..5893660 (+) 468 WP_062478450.1 Lrp/AsnC ligand binding domain-containing protein -
  RZE79_RS27660 (RZE79_27660) - 5893677..5894378 (-) 702 WP_319357696.1 YggS family pyridoxal phosphate-dependent enzyme -
  RZE79_RS27665 (RZE79_27665) pilT 5894430..5895473 (+) 1044 WP_056576373.1 type IV pilus twitching motility protein PilT Machinery gene
  RZE79_RS27670 (RZE79_27670) pilU 5895503..5896639 (+) 1137 WP_101493812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RZE79_RS27675 (RZE79_27675) - 5896705..5897613 (+) 909 WP_319357697.1 NAD(P)-dependent oxidoreductase -
  RZE79_RS27680 (RZE79_27680) - 5897759..5898553 (-) 795 WP_319357698.1 BON domain-containing protein -
  RZE79_RS27685 (RZE79_27685) - 5898550..5899200 (-) 651 WP_319357699.1 SIS domain-containing protein -
  RZE79_RS27690 (RZE79_27690) - 5899286..5899657 (-) 372 WP_319357700.1 YraN family protein -
  RZE79_RS27695 (RZE79_27695) rsmI 5899705..5900658 (+) 954 WP_319357701.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  RZE79_RS27700 (RZE79_27700) - 5900670..5901221 (-) 552 WP_319357702.1 RNA 2'-phosphotransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41782.85 Da        Isoelectric Point: 6.3136

>NTDB_id=903165 RZE79_RS27670 WP_101493812.1 5895503..5896639(+) (pilU) [Variovorax sp. EBFNA2]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVAMTKRGLTILVGATGSGKSTTLAAMVDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEGNAINFEEAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=903165 RZE79_RS27670 WP_101493812.1 5895503..5896639(+) (pilU) [Variovorax sp. EBFNA2]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGGGCTGGGCATGCCGCAGGTGCTCAAGGACGTGGCGATGACCAAGCGCGGCCTCACCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGGTCGACTGGCGCAACGAAAACTCCTACGGC
CACATCGTCACGGTGGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATTCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCCTTCGCCGAAACCGGTCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTGTCGCAACGGCTGGTGCCCACCGAAGACGGCCAGGGCCGCGTGGCGGCGGTCGAGATCCTGCTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAAATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGGGCAACGCCATCAACTTCGAAGAAGCGATCCGCAACGCCGA
TTCGGCCAACGACCTGCGCCTGCAGATCAAGCTGAACAGCCAGCGCGCGCGCAGCACCGACCTGGCGGCCGGCACCGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.324

92.328

0.585
  pilU Acinetobacter baylyi ADP1

57.263

94.709

0.542
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

44.377

87.037

0.386
  pilT Legionella pneumophila strain ERS1305867

44.377

87.037

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.47

87.831

0.373
  pilT Vibrio cholerae strain A1552

42.47

87.831

0.373