Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SE691_RS19145 Genome accession   NZ_CP138440
Coordinates   3981690..3982670 (-) Length   326 a.a.
NCBI ID   WP_058682188.1    Uniprot ID   -
Organism   Enterobacter cloacae strain 53EVA     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3976690..3987670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SE691_RS19115 - 3977566..3978063 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  SE691_RS19120 endA 3978158..3978865 (+) 708 WP_013098661.1 deoxyribonuclease I -
  SE691_RS19125 rsmE 3978918..3979649 (+) 732 WP_058682189.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SE691_RS19130 gshB 3979669..3980616 (+) 948 WP_023620782.1 glutathione synthase -
  SE691_RS19135 - 3980703..3981263 (+) 561 WP_013098664.1 YqgE/AlgH family protein -
  SE691_RS19140 ruvX 3981263..3981679 (+) 417 WP_319077564.1 Holliday junction resolvase RuvX -
  SE691_RS19145 pilT 3981690..3982670 (-) 981 WP_058682188.1 type IV pilus twitching motility protein PilT Machinery gene
  SE691_RS19150 - 3982688..3983389 (+) 702 WP_058682187.1 YggS family pyridoxal phosphate-dependent enzyme -
  SE691_RS19155 - 3983411..3983977 (+) 567 WP_013098667.1 YggT family protein -
  SE691_RS19160 yggU 3983974..3984261 (+) 288 WP_020687057.1 DUF167 family protein YggU -
  SE691_RS19165 - 3984274..3984867 (+) 594 WP_058680986.1 XTP/dITP diphosphatase -
  SE691_RS19170 hemW 3984860..3986008 (+) 1149 WP_058682186.1 radical SAM family heme chaperone HemW -
  SE691_RS19175 - 3986065..3986427 (+) 363 WP_072206145.1 endonuclease domain-containing protein -
  SE691_RS19180 - 3986495..3987211 (-) 717 WP_023621456.1 DUF2884 domain-containing protein -
  SE691_RS19185 - 3987268..3987594 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35682.68 Da        Isoelectric Point: 6.3038

>NTDB_id=902828 SE691_RS19145 WP_058682188.1 3981690..3982670(-) (pilT) [Enterobacter cloacae strain 53EVA]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRVGRLEPAPFPSPDVDALLKTWLNDEQQGAWWASGQVDFAVTLTGNQRLRA
SAFKQMKGNSITLRLLPRACPQLSALGVPRAIPELLSHDSGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQQREIGLHSPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLTQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=902828 SE691_RS19145 WP_058682188.1 3981690..3982670(-) (pilT) [Enterobacter cloacae strain 53EVA]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGTAGGCCGCCTTGAACCGGCTCCCTTTCCCTCTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCAGCAGGGGGCATGGTGGGCAAGCGGGCAGGTGGATTTTGCCGTGACCCTTACGGGAAACCAGCGTCTGCGCGCA
AGCGCCTTTAAACAGATGAAGGGCAACTCCATCACGCTACGCCTGTTGCCCCGGGCCTGTCCGCAGCTCTCCGCCCTGGG
TGTTCCCCGGGCTATCCCGGAACTCTTATCCCACGACAGTGGGCTGATACTGGTGACGGGGGCAACCGGCAGCGGCAAAT
CTACCACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTGGAAGATCCGGTGGAG
TTTGTCTATCAGAGCGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTGCG
CGCTGCGTTGCGTGAAGATCCGGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCCCTGACGG
CCGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGTGCCTCTCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGACGCAAAGACTGCTTCC
CGACCTGCAGGGTGGGCGCGTCGCGCTGTATGAACTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGGATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilB Haemophilus influenzae 86-028NP

33.427

100

0.365
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362