Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ANFP_RS01655 Genome accession   NZ_AP025160
Coordinates   319191..320240 (+) Length   349 a.a.
NCBI ID   WP_012536052.1    Uniprot ID   B7J408
Organism   Acidithiobacillus ferrooxidans strain NFP31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 314191..325240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ANFP_RS01625 (ANFP_03300) - 315387..315953 (+) 567 WP_009567390.1 acyloxyacyl hydrolase -
  ANFP_RS01630 (ANFP_03310) bioB 316000..316950 (-) 951 WP_012536051.1 biotin synthase BioB -
  ANFP_RS01635 (ANFP_03320) - 316947..317297 (-) 351 WP_009566572.1 DUF167 domain-containing protein -
  ANFP_RS01640 (ANFP_03330) - 317322..317600 (-) 279 WP_009566573.1 YggT family protein -
  ANFP_RS01645 (ANFP_03340) proC 317597..318424 (-) 828 WP_009566574.1 pyrroline-5-carboxylate reductase -
  ANFP_RS01650 (ANFP_03350) - 318421..319089 (-) 669 WP_009566576.1 YggS family pyridoxal phosphate-dependent enzyme -
  ANFP_RS01655 (ANFP_03360) pilT 319191..320240 (+) 1050 WP_012536052.1 type IV pilus twitching motility protein PilT Machinery gene
  ANFP_RS01660 (ANFP_03370) pilU 320250..321380 (+) 1131 WP_012536053.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ANFP_RS01665 (ANFP_03380) - 321386..322534 (+) 1149 WP_012536054.1 glycosyltransferase -
  ANFP_RS01670 (ANFP_03390) - 322594..323721 (-) 1128 WP_009565299.1 NAD(P)/FAD-dependent oxidoreductase -
  ANFP_RS01675 (ANFP_03400) - 323846..324463 (-) 618 WP_009565300.1 hypothetical protein -
  ANFP_RS01680 (ANFP_03410) sdo 324476..325165 (-) 690 WP_009565301.1 sulfur dioxygenase subunit alpha Sdo -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38344.78 Da        Isoelectric Point: 6.2844

>NTDB_id=90184 ANFP_RS01655 WP_012536052.1 319191..320240(+) (pilT) [Acidithiobacillus ferrooxidans strain NFP31]
MDISELLAFAVKNNASDTHISAGLAPMLRINGDIRPLNVEAQDSKTVHSMIYDIMNDFQRKGYEENLEIDFAYELPGVAR
FRVNAFNQDRGPAAAFRTIPASVLTLEDLNAPGSFKEIINVPRGLVLVTGPTGSGKSTTLAAMVDHINANRADHIITIED
PIEFLHTPKKCLINQREVGASTHSFENALRSALREDPDIILVGELRDLETMRLALTAAETGHIVFATLHTSSAPKTIDRI
VDSFPGGEKDMVRAMLSESLRAVISQTLLKTADGKGRVAAHEIMIATPAIRNLIRENKVAQMYSVIQTGQNQGMQTLDQC
LADLVRAHKVTHEDALRRVQNKDSFMNVA

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=90184 ANFP_RS01655 WP_012536052.1 319191..320240(+) (pilT) [Acidithiobacillus ferrooxidans strain NFP31]
ATGGATATCTCAGAACTGCTCGCCTTCGCGGTGAAAAACAACGCGTCGGACACCCATATCTCGGCAGGTCTGGCGCCGAT
GCTGCGGATCAATGGCGACATCCGTCCGCTGAATGTGGAGGCGCAGGACAGCAAAACCGTACACAGCATGATTTACGACA
TCATGAACGATTTCCAGCGGAAGGGGTATGAGGAGAACCTGGAAATCGACTTTGCCTATGAACTGCCGGGTGTGGCGCGT
TTTCGGGTTAACGCCTTCAACCAGGACCGCGGACCGGCTGCGGCGTTCCGGACGATTCCTGCGAGTGTGCTGACACTGGA
AGACCTCAATGCGCCCGGTTCTTTCAAGGAGATCATCAACGTGCCGCGCGGACTGGTATTGGTCACGGGGCCGACCGGAT
CGGGTAAGTCAACGACGCTGGCAGCGATGGTGGATCATATCAATGCGAATCGCGCCGACCACATCATTACCATTGAAGAT
CCCATCGAATTTCTGCACACCCCCAAGAAGTGCCTGATCAACCAGCGCGAGGTGGGTGCCAGCACCCACTCCTTTGAAAA
CGCCCTGCGCTCGGCTTTACGTGAAGATCCCGATATCATTCTGGTGGGCGAGCTACGTGATCTGGAGACCATGCGCCTGG
CGCTGACCGCGGCAGAGACCGGCCATATCGTGTTTGCGACGCTGCATACCAGTTCCGCACCAAAAACCATCGACCGTATT
GTCGATTCCTTTCCCGGGGGGGAAAAGGATATGGTCCGCGCCATGCTGTCCGAATCCCTGCGCGCGGTCATTTCCCAGAC
TCTGCTCAAAACGGCCGACGGCAAGGGAAGGGTGGCCGCGCACGAAATCATGATCGCCACCCCGGCTATCCGCAACCTGA
TTCGTGAGAACAAGGTCGCGCAGATGTACTCGGTGATCCAGACCGGGCAAAATCAGGGTATGCAAACCCTGGACCAGTGT
CTTGCCGATCTGGTGCGTGCCCACAAGGTGACTCACGAAGATGCGCTGCGCCGGGTACAGAACAAAGACAGCTTCATGAA
CGTGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7J408

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

69.075

99.14

0.685
  pilT Acinetobacter baumannii D1279779

69.075

99.14

0.685
  pilT Acinetobacter baumannii strain A118

69.075

99.14

0.685
  pilT Pseudomonas stutzeri DSM 10701

67.826

98.854

0.67
  pilT Pseudomonas aeruginosa PAK

67.246

98.854

0.665
  pilT Acinetobacter baylyi ADP1

70.769

93.123

0.659
  pilT Legionella pneumophila strain Lp02

65.507

98.854

0.648
  pilT Legionella pneumophila strain ERS1305867

65.507

98.854

0.648
  pilT Vibrio cholerae strain A1552

69.231

93.123

0.645
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.231

93.123

0.645
  pilT Neisseria meningitidis 8013

65.217

98.854

0.645
  pilT Neisseria gonorrhoeae MS11

64.928

98.854

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.143

100

0.501
  pilU Pseudomonas stutzeri DSM 10701

42.857

96.275

0.413
  pilU Acinetobacter baylyi ADP1

42.308

96.848

0.41
  pilU Vibrio cholerae strain A1552

39.881

96.275

0.384

Multiple sequence alignment