Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ANFP_RS01660 Genome accession   NZ_AP025160
Coordinates   320250..321380 (+) Length   376 a.a.
NCBI ID   WP_012536053.1    Uniprot ID   B7J409
Organism   Acidithiobacillus ferrooxidans strain NFP31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 315250..326380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ANFP_RS01625 (ANFP_03300) - 315387..315953 (+) 567 WP_009567390.1 acyloxyacyl hydrolase -
  ANFP_RS01630 (ANFP_03310) bioB 316000..316950 (-) 951 WP_012536051.1 biotin synthase BioB -
  ANFP_RS01635 (ANFP_03320) - 316947..317297 (-) 351 WP_009566572.1 DUF167 domain-containing protein -
  ANFP_RS01640 (ANFP_03330) - 317322..317600 (-) 279 WP_009566573.1 YggT family protein -
  ANFP_RS01645 (ANFP_03340) proC 317597..318424 (-) 828 WP_009566574.1 pyrroline-5-carboxylate reductase -
  ANFP_RS01650 (ANFP_03350) - 318421..319089 (-) 669 WP_009566576.1 YggS family pyridoxal phosphate-dependent enzyme -
  ANFP_RS01655 (ANFP_03360) pilT 319191..320240 (+) 1050 WP_012536052.1 type IV pilus twitching motility protein PilT Machinery gene
  ANFP_RS01660 (ANFP_03370) pilU 320250..321380 (+) 1131 WP_012536053.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ANFP_RS01665 (ANFP_03380) - 321386..322534 (+) 1149 WP_012536054.1 glycosyltransferase -
  ANFP_RS01670 (ANFP_03390) - 322594..323721 (-) 1128 WP_009565299.1 NAD(P)/FAD-dependent oxidoreductase -
  ANFP_RS01675 (ANFP_03400) - 323846..324463 (-) 618 WP_009565300.1 hypothetical protein -
  ANFP_RS01680 (ANFP_03410) sdo 324476..325165 (-) 690 WP_009565301.1 sulfur dioxygenase subunit alpha Sdo -
  ANFP_RS01685 (ANFP_03420) - 325306..325971 (-) 666 WP_215905594.1 Crp/Fnr family transcriptional regulator -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41500.89 Da        Isoelectric Point: 5.9956

>NTDB_id=90185 ANFP_RS01660 WP_012536053.1 320250..321380(+) (pilU) [Acidithiobacillus ferrooxidans strain NFP31]
MSVLDDLLVLMVEKNGSDLYLTVGSPPVMKVDGRTVPLGTEMLKPGQVLNLAKEILGMERLQEFQHEKEINMAISVPGVG
RFRVNGFFQRGELSFVLRAIKTQIPSLEQLRLPPILKDLAMTSRGLVLFVGSTGSGKSTSLASMVQYRNQNAAGHILTIE
DPIEFLHRNAKSIVNQREVGIDTLNYERALENALREAPDVILIGEVRSRDTMDHAIAYAETGHLCMSTLHANNANQAIER
IINFFPEDRKKQLLMDLSLNLRAVVSQRLLPVKGQSGRIAAMEVLINTPAIADLIYKGEVGLLKDAMSRTNDVGMQTFDQ
SLLQLFMDGLVEYDDALRAADSQNDLRLAIKQACMRSGMADPGAATSAEGQWRIQS

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=90185 ANFP_RS01660 WP_012536053.1 320250..321380(+) (pilU) [Acidithiobacillus ferrooxidans strain NFP31]
ATGTCTGTGCTGGATGACCTGCTGGTCCTGATGGTGGAAAAAAACGGCTCGGATCTGTACCTCACCGTGGGCTCGCCACC
GGTCATGAAAGTCGACGGACGGACGGTTCCGCTGGGGACAGAAATGCTGAAACCGGGTCAGGTGCTGAATCTGGCCAAGG
AAATTCTGGGGATGGAGCGCTTGCAGGAGTTCCAGCACGAGAAAGAAATCAACATGGCCATTTCCGTGCCCGGGGTGGGG
CGTTTCCGGGTCAACGGGTTTTTCCAGCGTGGTGAACTGAGCTTTGTCCTGCGCGCCATCAAGACCCAGATTCCCAGCCT
GGAGCAGCTCAGGCTGCCGCCGATACTGAAAGATCTGGCCATGACGTCGCGGGGGCTGGTGCTCTTCGTCGGTTCTACCG
GTTCCGGGAAAAGCACCTCTCTGGCGTCGATGGTCCAGTATCGCAACCAGAACGCGGCGGGTCATATCCTCACCATCGAG
GATCCGATCGAATTTCTGCACCGGAATGCGAAGTCCATCGTCAATCAGCGCGAAGTGGGCATCGATACCCTGAATTATGA
GCGGGCGTTGGAGAATGCATTGCGGGAAGCACCGGATGTCATCCTGATCGGCGAAGTGCGCAGCCGGGACACCATGGATC
ATGCCATCGCTTATGCCGAAACTGGGCACCTGTGTATGTCCACCCTCCATGCGAACAATGCCAATCAGGCCATTGAACGG
ATCATCAATTTTTTCCCGGAAGACCGTAAAAAACAGTTGCTGATGGACCTTTCGCTCAATTTGCGCGCCGTGGTCTCACA
GCGCCTGTTGCCGGTCAAGGGGCAGAGCGGACGAATCGCTGCAATGGAGGTGCTGATCAACACTCCGGCCATTGCCGATC
TGATCTACAAGGGTGAAGTGGGCCTGCTGAAGGACGCCATGTCGCGAACGAACGACGTCGGTATGCAGACGTTTGATCAG
AGCCTGTTGCAGTTGTTTATGGATGGACTGGTGGAATATGACGATGCCCTGCGCGCGGCCGATTCGCAGAACGATCTGCG
CCTCGCCATCAAACAGGCGTGTATGCGTTCGGGCATGGCAGATCCCGGGGCCGCCACCTCTGCGGAAGGGCAGTGGCGTA
TTCAGTCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7J409

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.195

92.553

0.548
  pilU Vibrio cholerae strain A1552

53.736

92.553

0.497
  pilU Acinetobacter baylyi ADP1

53.582

92.819

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.642

92.021

0.402
  pilT Vibrio cholerae strain A1552

43.642

92.021

0.402
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.939

92.287

0.396
  pilT Acinetobacter baylyi ADP1

42.183

90.16

0.38
  pilT Acinetobacter baumannii D1279779

41.543

89.628

0.372
  pilT Acinetobacter nosocomialis M2

41.543

89.628

0.372
  pilT Acinetobacter baumannii strain A118

41.543

89.628

0.372
  pilT Legionella pneumophila strain Lp02

41.791

89.096

0.372
  pilT Legionella pneumophila strain ERS1305867

41.791

89.096

0.372
  pilT Pseudomonas stutzeri DSM 10701

41.246

89.628

0.37
  pilT Pseudomonas aeruginosa PAK

41.246

89.628

0.37
  pilT Neisseria meningitidis 8013

39.535

91.489

0.362

Multiple sequence alignment