Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TthTMY_RS00090 Genome accession   NZ_AP025158
Coordinates   17596..18681 (-) Length   361 a.a.
NCBI ID   WP_096411375.1    Uniprot ID   -
Organism   Thermus thermophilus strain TMY     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 12596..23681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TthTMY_RS00075 (TthTMY_00120) - 14540..15172 (-) 633 WP_096411372.1 histidine phosphatase family protein -
  TthTMY_RS00080 (TthTMY_00130) purM 15169..16170 (-) 1002 WP_096411373.1 phosphoribosylformylglycinamidine cyclo-ligase -
  TthTMY_RS00085 (TthTMY_00140) gatB 16176..17585 (-) 1410 WP_096411374.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  TthTMY_RS00090 (TthTMY_00150) pilT 17596..18681 (-) 1086 WP_096411375.1 type IV pilus twitching motility protein PilT Machinery gene
  TthTMY_RS00095 (TthTMY_00160) pilF 18694..21363 (-) 2670 WP_096411376.1 type IV pilus assembly ATPase PilB Machinery gene
  TthTMY_RS00100 (TthTMY_00170) - 21360..21845 (-) 486 WP_096411377.1 YqeG family HAD IIIA-type phosphatase -
  TthTMY_RS00105 (TthTMY_00180) pgeF 21880..22608 (-) 729 WP_096411378.1 peptidoglycan editing factor PgeF -
  TthTMY_RS00110 (TthTMY_00190) surE 22605..23360 (-) 756 WP_096411379.1 5'/3'-nucleotidase SurE -
  TthTMY_RS00115 (TthTMY_00200) - 23419..23625 (-) 207 WP_096411380.1 cold-shock protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39864.22 Da        Isoelectric Point: 7.5111

>NTDB_id=90157 TthTMY_RS00090 WP_096411375.1 17596..18681(-) (pilT) [Thermus thermophilus strain TMY]
MAKTPDVVDLLALAVERGASDLVITVGLPPMLKIDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPSTVKSFEELGLPKVIADIAMSPRGLVLVTGPTGSGKSTTLAAMIDYINERKACHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
VDRIVDVFPEAQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMIT
MDACLADLYKRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=90157 TthTMY_RS00090 WP_096411375.1 17596..18681(-) (pilT) [Thermus thermophilus strain TMY]
ATGGCCAAAACCCCGGACGTGGTGGACCTTTTGGCGCTTGCGGTGGAGCGGGGGGCGAGCGACCTGGTGATCACCGTGGG
CCTGCCCCCCATGCTGAAGATCGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAGCGGATCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGGAAGGGGCGGTACCGGGTCAACGTCTTCCTGCAACGGGGGAGCGTGGGGGGGGTCTTGAGGGTGGTGCCGAGCACCGT
GAAGAGCTTTGAGGAGCTCGGCCTCCCCAAGGTCATCGCCGACATCGCCATGAGCCCCCGGGGCCTCGTCCTGGTCACGG
GGCCCACAGGGTCGGGCAAGAGCACCACCCTGGCCGCCATGATCGACTACATCAACGAACGCAAGGCCTGCCACATCGTC
ACCATTGAGGACCCCATAGAGTTCTTCCACAAGCACAAACGGGCCATCGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAGCTTCCACAAGGCGCTCCGCAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTCGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACGGGGCACCTGGTCATGGGCACCCTCCACACCAACTCCGCCCCGGAAACG
GTAGACCGCATCGTGGACGTCTTCCCGGAGGCCCAGCAGGAACAGGTCCGGGTGCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACGCAACAACTCCTGCCCAAGGCCTTTGGCGGCGGGCGGGTCCTGGCCTACGAGCTCATGATCGCCACCCCGGCCG
TGAGGGCCCTCATCCGGGAGGGGAAGAGCCACCAGTTAAGGAGCGTGATCCAGACGGGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTCGCCGACCTGTACAAGCGCAAGCTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.705

97.784

0.662
  pilT Pseudomonas aeruginosa PAK

54.36

95.291

0.518
  pilT Acinetobacter baylyi ADP1

54.07

95.291

0.515
  pilT Pseudomonas stutzeri DSM 10701

54.07

95.291

0.515
  pilT Acinetobacter baumannii D1279779

53.043

95.568

0.507
  pilT Acinetobacter baumannii strain A118

53.043

95.568

0.507
  pilT Acinetobacter nosocomialis M2

53.043

95.568

0.507
  pilT Neisseria meningitidis 8013

53.254

93.629

0.499
  pilT Neisseria gonorrhoeae MS11

52.522

93.352

0.49
  pilT Legionella pneumophila strain ERS1305867

50.291

95.291

0.479
  pilT Legionella pneumophila strain Lp02

50.291

95.291

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.672

91.136

0.471
  pilT Vibrio cholerae strain A1552

51.672

91.136

0.471
  pilU Pseudomonas stutzeri DSM 10701

47.23

95.014

0.449
  pilU Vibrio cholerae strain A1552

44.611

92.521

0.413
  pilU Acinetobacter baylyi ADP1

42.029

95.568

0.402

Multiple sequence alignment